RAPID'Sakazakii Medium

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RAPID'<I>Sakazakii</I>

Overview

Key Benefits
  • Improved formulation results in big easy-to-read colored colonies
  • Short protocol eliminates the need for secondary enrichment
  • Results a full day earlier provides a time and cost savings
  • Ability to detect atypical strains
  • Good correlation with ISO method
Protocol
Detection of Chronobacter spp.
Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


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Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Selective chromogenic medium for detection of Cronobacter spp. (formerly Enterobacter sakazakii).
Read full description

Ordering Information


Description

Talk with a Specialist

RAPID'Sakazakii Medium is a selective chromogenic agar that is intended for the isolation and detection of Cronobacter spp. (formerly classified as Enterobacter sakazakii). The most common source of Cronobacter spp. contamination is powdered infant formula.

Key Benefits

  • Easy-to-read chromogenic reaction
    • Blue to blue-green colonies
  • Cost-effective short protocol
    • No selective enrichment in mLST + Vancomycin
    • Negative results in less than 42 hr
  • Validation
    • Certified NF VALIDATION according to the ISO 16140:2003 standard for the detection of Cronobacterspp. in infant milk powders

Principle

Detection is based on a specific enzymatic activity common to all species of Cronobacter. Hydrolysis of the chromogenic substrate 5-bromo-4-chloro-3-indol α-D-glucopyranoside leads to the formation of typical turquoise-colored colonies.

The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, giving the medium increased selectivity. Competing bacteria, if not completely inhibited, will form light purple or gray colonies.

RAPID'Sakazakii Chromogenic Assay

Rapid Sakazakii
Enzymatic Activity
Cronobacter
spp.

Other
Enterobacteriaceae
α-D-Glucosidase + -
     

Simple and Rapid Protocol

RAPID'Sakazakii Medium is formulated for use in standard methods (for example, the ISO 22964 reference method). The medium can also be used with a shortened protocol to test “clean” samples such as powdered infant formula with a low level of contamination. The short protocol is used to detect Cronobacter spp. in 24 hr after an 18-hr enrichment in buffered peptone water, eliminating the need for selective enrichment.

Ordering Information

RAPID'Sakazakii Agar is offered as ready-to-use 90 mm plates (3563971) or as a dehydrated agar base (3564976)


Specifications

Assay type Chromogenic media
Organism Cronobacter, Enterobacter sakazakii

Ordering

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RAPID'Sakazakii Agar Plates

3563971
Pkg of 20, 90 mm agar plates

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$76.00 
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RAPID'Sakazakii Agar, dehydrated

3564976
Pkg of 1, 500 g of dehydrated agar base, enough for 13.6 L of medium

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$758.00 
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PIF Supplement

12013322
Pkg of 1, 2 g vial

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$293.00 
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Trypto-Casein-Soy Agar (TSA) tubes

3555944
Pkg of 25, 8 ml slanted culture tubes

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$32.00 
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Trypto-Casein-Soy Agar (TSA), dehydrated

3564554
Pkg of 1, 500 g of dehydrated agar base

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$78.00 
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iQ-Check Cronobacter PCR Detection Kit

3578137
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Buffered Peptone Water Plus

3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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$28.00 
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Buffered Peptone Water Plus

3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

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$113.00 
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Buffered Peptone Water Plus

3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

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$114.00 
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Buffered Peptone Water Plus

3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

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$78.00 
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Buffered Peptone Water Plus

3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

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$716.00 
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Buffered Peptone Water Standard

12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions

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$99.00 
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Buffered Peptone Water Standard, dehydrated

12013259
Pkg of 1, 500 g — for standard enrichment conditions

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$68.00 
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Buffered Peptone Water Standard, dehydrated

12013258
Pkg of 1, 5 kg — for standard enrichment conditions

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$622.00 
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Documents

Number Description Options
10000127307 RAPID'Sakazakii, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
10000126749 PIF Supplement, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
7381 PIF Supplement flyer Click to download
FSD_16288 RAPID’Chromogenic Media Brochure Click to download
FSD_17933 Food Safety and Quality Reference Guide Click to download
7274 Complete Solutions for Service Laboratories Click to download
10000128740 Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
10000128738 Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download

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