RAPID'Sakazakii Medium is a selective chromogenic agar that is intended for the isolation and detection of Cronobacter spp. (formerly classified as Enterobacter sakazakii). The most common source of Cronobacter spp. contamination is powdered infant formula.
- Easy-to-read chromogenic reaction
- Blue to blue-green colonies
- Cost-effective short protocol
- No selective enrichment in mLST + Vancomycin
- Negative results in less than 42 hr
- Certified NF VALIDATION according to the ISO 16140:2003 standard for the detection of Cronobacterspp. in infant milk powders
Detection is based on a specific enzymatic activity common to all species of Cronobacter. Hydrolysis of the chromogenic substrate 5-bromo-4-chloro-3-indol α-D-glucopyranoside leads to the formation of typical turquoise-colored colonies.
The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, giving the medium increased selectivity. Competing bacteria, if not completely inhibited, will form light purple or gray colonies.
RAPID'Sakazakii Chromogenic Assay
Simple and Rapid Protocol
RAPID'Sakazakii Medium is formulated for use in standard methods (for example, the ISO 22964 reference method). The medium can also be used with a shortened protocol to test “clean” samples such as powdered infant formula with a low level of contamination. The short protocol is used to detect Cronobacter spp. in 24 hr after an 18-hr enrichment in buffered peptone water, eliminating the need for selective enrichment.
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