RAPID'Sakazakii Medium

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Overview

Selective chromogenic medium for detection of Cronobacter spp. (formerly Enterobacter sakazakii).

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Description

Description

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RAPID'Sakazakii Medium is a selective chromogenic agar that is intended for the isolation and detection of Cronobacter spp. (formerly classified as Enterobacter sakazakii). The most common source of Cronobacter spp. contamination is powdered infant formula.

Key Benefits

  • Easy-to-read chromogenic reaction
    • Blue to blue-green colonies
  • Cost-effective short protocol
    • No selective enrichment in mLST + Vancomycin
    • Negative results in less than 42 hr
  • Validation
    • Certified NF VALIDATION according to the ISO 16140:2003 standard for the detection of Cronobacterspp. in infant milk powders

Principle

Detection is based on a specific enzymatic activity common to all species of Cronobacter. Hydrolysis of the chromogenic substrate 5-bromo-4-chloro-3-indol α-D-glucopyranoside leads to the formation of typical turquoise-colored colonies.

The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, giving the medium increased selectivity. Competing bacteria, if not completely inhibited, will form light purple or gray colonies.

RAPID'Sakazakii Chromogenic Assay

Rapid Sakazakii
Enzymatic Activity
Cronobacter
spp.

Other
Enterobacteriaceae
α-D-Glucosidase + -
     

Simple and Rapid Protocol

RAPID'Sakazakii Medium is formulated for use in standard methods (for example, the ISO 22964 reference method). The medium can also be used with a shortened protocol to test “clean” samples such as powdered infant formula with a low level of contamination. The short protocol is used to detect Cronobacter spp. in 24 hr after an 18-hr enrichment in buffered peptone water, eliminating the need for selective enrichment.

Ordering Information

RAPID'Sakazakii Agar is offered as ready-to-use 90 mm plates (3563971) or as a dehydrated agar base (3564976)

Specifications

Specifications

Ordering

Ordering

items

Use the filters below to refine results!

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3563971
Pkg of 20, 90 mm agar plates
3564976
Pkg of 1, 500 g of dehydrated agar base, enough for 13.6 L of medium
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(Discontinued) PIF Supplement
12013322

Pkg of 1, 2 g vial

3555944
Pkg of 25, 8 ml slanted culture tubes
3564554
Pkg of 1, 500 g of dehydrated agar base
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3578137
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
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3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions
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3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions
356-4684_160.jpg
3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
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3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions
3555795_th.jpg
12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions
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12013259
Pkg of 1, 500 g — for standard enrichment conditions
12013258_th.jpg
12013258
Pkg of 1, 5 kg — for standard enrichment conditions

Documents

Documents

Number Description Options
10000127307
RAPID'Sakazakii, User Guide (English, French, German, Italian, Portuguese, Spanish)
3260
Performance summary, RAPID'Sakazakii
10000126749
PIF Supplement, User Guide (English, French, German, Italian, Portuguese, Spanish)
7381
PIF Supplement flyer
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)