RAPID'Sakazakii Medium

RAPID'Sakazakii Medium

Selective chromogenic medium for detection of Cronobacter spp. (formerly Enterobacter sakazakii).

 

Validation

 

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Ordering Information

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Overview

RAPID'Sakazakii Medium is a selective chromogenic agar that is intended for the isolation and detection of Cronobacter spp. (formerly classified as Enterobacter sakazakii). The most common source of Cronobacter spp. contamination is powdered infant formula.

Key Benefits

Easy-to-read chromogenic reaction

  • Blue to blue-green colonies

Cost-effective short protocol

  • No selective enrichment in mLST + Vancomycin
  • Negative results in less than 42 hr

Validation

  • Certified NF VALIDATION according to the ISO 16140:2003 standard for the detection of Cronobacter spp. in infant milk powders

Principle

Detection is based on a specific enzymatic activity common to all species of Cronobacter. Hydrolysis of the chromogenic substrate 5-bromo-4-chloro-3-indol α-D-glucopyranoside leads to the formation of typical turquoise-colored colonies.

The medium includes compounds carefully selected to promote the growth of Cronobacter spp. while suppressing interfering flora, giving the medium increased selectivity. Competing bacteria, if not completely inhibited, will form light purple or gray colonies.

RAPID'Sakazakii Chromogenic Assay

Rapid Sakazakii
Enzymatic Activity
Cronobacter spp.
Other Enterobacteriaceae
α-D-Glucosidase yes no

Simple and Rapid Protocol

RAPID'Sakazakii Medium is formulated for use in standard methods (for example, the ISO 22964 reference method). The medium can also be used with a shortened protocol to test “clean” samples such as powdered infant formula with a low level of contamination. The short protocol is used to detect Cronobacter spp. in 24 hr after an 18-hr enrichment in buffered peptone water, eliminating the need for selective enrichment.

Ordering Information

RAPID'Sakazakii Agar is offered as ready-to-use 90 mm plates (3563971) or as a dehydrated agar base (3564976)

Specifications

Ordering

items

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3563971
Pkg of 20, 90 mm agar plates
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iQ_Check_Salmonella_II_Kit_2019_SKU_TN.jpg
3578137
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3554179

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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3564684

Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Documents

Number Description Options
10000172873
RAPID'Sakazakii, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
3260
Performance summary, RAPID'Sakazakii
10000126749
PIF Supplement, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
7381
PIF Supplement flyer
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7408
Chromogenic Media Reference Guide
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)