Foresight Chromatography RoboColumn Units

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Foresight Chromatography RoboColumn Units

Overview

Foresight™ Prepacked Chromatography Plates and Columns Selection Guide
Foresight Plates

Foresight plates are 96-well filter plates prepacked with 20 µl of chromatography media in each well.

Foresight RoboColumn Units

Foresight RoboColumn units are offered as single rows of eight prepacked microcolumns. Foresight RoboColumn units are ideal for high-throughput (HT) process development.

Foresight Columns

Foresight columns are ideal for confirming the operational window obtained through design of experiments (DOE) in trials with Foresight plates and/or Foresight RoboColumn units.

Applications

Foresight plates can be used for HT evaluation of various binding, elution, or wash conditions for different chromatography media. They can be processed using manual or automated robotic systems, and sample collection may be achieved through centrifugation or vacuum manifold techniques.

Foresight RoboColumn units are fully compatible with robotic liquid handling systems, allowing process developers to perform automated HT parallel media screening and/or small-scale method development. Multiple rows up to 12 can be combined to form a 96-column array, allowing users to evaluate various chromatography media and conditions in parallel.

Foresight columns may be used for media screening experiments and/or small-scale method development. Foresight columns have standard HPLC connections, which make them compatible with the NGC™ chromatography system and other liquid chromatography systems.

Physical Dimensions

Standard 96-well plate format
128 × 86 × 33 mm

1 × 8 row
128 × 86 × 14 mm

8 × 20 mm (1-ml column)
8 × 100 mm (5-ml column)

Working Volume

<900 µl

200–600 µl

1–5 ml

Recommended Flow Rate, cm/hr

N/A

16–1,000

59.5-119 (1-ml column)
287.5-595 (5-ml column)

Maximum Pressure, bar (psi)

N/A

8 (116)

30 (435)

Media Types Available

UNOsphere™ Q, UNOsphere S, UNOsphere Rapid S; Nuvia™ Q, Nuvia S, Nuvia™ cPrime™; CHT™ Type I, CHT Type II

Ordering Information
Foresight™ Filter Plates and Columns Workflow

Foresight chromatography filter plates and columns are prepacked with a range of Bio-Rad’s process chromatography media, offering process scientists convenience and reliability for their high-throughput (HT) experimentation needs. Their robust design allows process scientists to use the prepacked formats through the entire purification development cycle, from HT media screening to small-scale methods development and scale-up optimization.
Learn more


Purification scale-up workflow shows the use of HT screening and design of experiments (DOE) to define an operational window for biomanufacturing.

 

Using High-Throughput (HT) Methods to Accelerate DOE

High-throughput experimentation (HTE) was used in this case study in concert with design of experiments (DOE) methodology to study the mass recovery of a basic therapeutic protein (pI 9.2–9.5) using a hydrophobic cation exchange mixed-mode resin.

Foresight RoboColumn units are prepacked chromatography microcolumns used for rapid process development. They may be used for parallel media screening experiments and/or small-scale method development.
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Ordering Information


Description

Resin Format Guide

Foresight RoboColumn Units are prepacked chromatography microcolumns used for rapid process development. They may be used for parallel media screening experiments and/or small-scale method development. Their design is fully compatible with robotic liquid handling workstations, such as the Freedom EVOware series from Tecan, allowing process developers to perform high-throughput (HT) parallel media screening and experimentation. Through design of experiments (DOE) utilizing Foresight RoboColumn Units, an operational window can be defined to aid in biomanufacturing processes.

Features and Benefits:

  • Convenience — prepacked columns used in conjunction with a robotic liquid handling device allow users to create automated protocols for HT experimentation. The ability to define a design space enables process scientists to develop robust purification processes
  • Reliability — each column is individually packed under optimum compression, ensuring consistent experimental results
  • Efficiency — small column design enables using precious feed sample more efficiently while performing real chromatographic separations in parallel to determine critical quality parameters

The Foresight Instruction Manual describes how to use Foresight RoboColumn units prepacked with Bio-Rad chromatography media.

Chromatography Type

Foresight 200 µl Robocolumns

Foresight 600 µl Robocolumns
Strong Anion Exchange
7324819
UNOsphere Q RoboColumn Unit
7324820
UNOsphere Q RoboColumn Unit
Strong Anion Exchange
12007013
Nuvia HP-Q RoboColumn Unit
12007014
Nuvia HP-Q RoboColumn Unit
Strong Anion Exchange
7324804
Nuvia Q RoboColumn Unit
7324805
Nuvia Q RoboColumn Unit
Strong Cation Exchange
7324813
UNOsphere S RoboColumn Unit
7324814
UNOsphere S RoboColumn Unit
Strong Cation Exchange
7324831
Nuvia HR-S RoboColumn Unit
7324832
Nuvia HR-S RoboColumn Unit
Strong Cation Exchange
7324801
Nuvia S RoboColumn Unit
7324802
Nuvia S RoboColumn Unit
Metal Affinity
12004051
Nuvia IMAC Ni-Charged RoboColumn Unit
12004052
Nuvia IMAC Ni-Charged RoboColumn Unit
Mixed-Mode:
Hydrophobic Anionic
12007394
Nuvia aPrime 4A RoboColumn Unit
12007395
Nuvia aPrime 4A RoboColumn Unit
Mixed-Mode:
Hydrophobic Cationic
7324807
Nuvia cPrime RoboColumn Unit
7324808
Nuvia cPrime RoboColumn Unit
Mixed-Mode:
Cationic, Metal affinity
7324822
CHT Type I RoboColumn Unit, 40 µm
7324823
CHT Type I RoboColumn Unit, 40 µm
Mixed-Mode:
Cationic, Metal affinity
7324825
CHT Type II RoboColumn Unit, 40 µm
7324826
CHT Type II RoboColumn Unit, 40 µm
Mixed-Mode:
Cationic, Metal affinity
12003152
CHT XT RoboColumn Unit, 40 µm
12003148
CHT XT RoboColumn Unit, 40 µm
Mixed-Mode:
Cationic, Metal affinity
7324828
MPC Type I RoboColumn Unit, 40 µm
7324829
MPC Type I RoboColumn Unit, 40 µm

Other formats available:

Learn more about Foresight Prepacked Collumn and Plate formats and applications.


Specifications

Plate size, mm 128.3 x 86.0 x 14.0
Plate material Polyoxymethylene copolymer
Column inner diameter, mm 5
Column material Polypropylene
Storage solution Nuvia™ cPrime™ and Nuvia IMAC Media: 20% ethanol
MPC™ and CHT Type I or Type II Media — 40 µm particle size: 20% ethanol + 0.01 M NaH2PO4, pH 6.8
Nuvia Q & S, UNOsphere Q & S: 20% EtOH + 150 mM NaCl
Storage temperature, °C Recommended: 4–30
Working temperature, °C 4–30
Laser label Placed on every individual column body; label includes product number, media name, column volume in µl

Ordering

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Foresight Nuvia HP-Q RoboColumn, 200 μl

12007013
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size

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$440.00 
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Foresight Nuvia HP- Q RoboColumn, 600 μl

12007014
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia HP-Q Strong Anion Exchange Chromatography Media, 50 µm particle size

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$525.00 
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Foresight Nuvia aPrime 4A RoboColumn Unit, 200 µl

12007394
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with Nuvia aPrime 4A, 50 µm particle size

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$415.00 
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Foresight Nuvia aPrime 4A RoboColumn, 600 µl

12007395
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with Nuvia aPrime 4A, 50 µm particle size

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$505.00 
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Foresight™ CHT™ XT RoboColumn, 200 µl

12003152
Pkg of 1, row of 8 columns, 200 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size

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$380.00 
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Foresight™ CHT™ XT RoboColumn, 600 µl

12003148
Pkg of 1, row of 8 columns, 600 µl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite XT Mixed-Mode Media, 40 µm particle size

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$440.00 
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Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 200 µl

12004051
1 row of 8 columns, 200 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin

List Price: 
 
$445.00 
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Foresight™ Nuvia™ Ni-Charged IMAC RoboColumn Unit, 600 µl

12004052
1 row of 8 columns, 600 μl, RoboColumn Unit prepacked with Nuvia IMAC Affinity Chromatography Resin

List Price: 
 
$520.00 
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Foresight™ MPC™ Type I RoboColumn Unit, 40 µm, 200 µl

7324828
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with MPC Type I Mixed-Mode Chromatography Resin, 40 µm particle size

List Price: 
 
$360.00 
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Foresight™ MPC™ Type I RoboColumn Unit, 40 µm, 600 µl

7324829
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with MPC Type I Mixed-Mode Chromatography Resin, 40 µm particle size

List Price: 
 
$410.00 
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Foresight™ Nuvia™ HR-S RoboColumn Unit, 200 µl

7324831
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin

List Price: 
 
$325.00 
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Foresight™ Nuvia™ HR-S RoboColumn Unit, 600 µl

7324832
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin

List Price: 
 
$405.00 
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Foresight™ UNOsphere SUPrA™ RoboColumn Unit, 200 µl

7324834
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with UNOsphere SUPrA Affinity Chromatography Resin

List Price: 
 
$525.00 
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Foresight™ UNOsphere SUPrA™ RoboColumn Unit, 600 µl

7324835
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with UNOsphere SUPrA Affinity Chromatography Resin

List Price: 
 
$665.00 
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Foresight™ UNOsphere™ Q RoboColumn Unit

7324819
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with UNOsphere Q Strong Anion Exchange Chromatography Media

List Price: 
 
$340.00 
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Foresight™ UNOsphere™ Q RoboColumn Unit

7324820
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with UNOsphere Q strong anion exchange chromatography media

List Price: 
 
$380.00 
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Foresight™ UNOsphere™ S RoboColumn Unit

7324813
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with UNOsphere S Strong Cation Exchange Chromatography Media

List Price: 
 
$315.00 
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Foresight™ UNOsphere™ S RoboColumn Unit

7324814
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with UNOsphere S Strong Cation Exchange Chromatography Media

List Price: 
 
$380.00 
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Foresight™ Nuvia™ Q RoboColumn Unit

7324804
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia Q Strong Anion Exchange Chromatography Media

List Price: 
 
$335.00 
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Foresight™ Nuvia™ Q RoboColumn Unit

7324805
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia Q Strong Anion Exchange Chromatography Media

List Price: 
 
$400.00 
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Foresight™ Nuvia™ S RoboColumn Unit

7324801
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia S Strong Cation Exchange Chromatography Media

List Price: 
 
$350.00 
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Foresight™ Nuvia™ S RoboColumn Unit

7324802
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia S Strong Cation Exchange Chromatography Media

List Price: 
 
$420.00 
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Foresight™ Nuvia™ cPrime™ RoboColumn Unit

7324807
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

List Price: 
 
$395.00 
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Foresight™ Nuvia™ cPrime™ RoboColumn Unit

7324808
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

List Price: 
 
$440.00 
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Foresight™ CHT™ Type I RoboColumn Unit

7324822
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite Type I Multimodal Chromatography Media, 40 μm particle size

List Price: 
 
$360.00 
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Foresight™ CHT™ Type I RoboColumn Unit

7324823
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite Type I Multimodal Chromatography Media, 40 μm particle size

List Price: 
 
$440.00 
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Foresight CHT Type II RoboColumn Unit

7324825
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size

List Price: 
 
$360.00 
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Foresight™ CHT™ Type II RoboColumn Unit

7324826
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size

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$440.00 
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Documents

Number Description Options
10027565 Instruction Manual, Foresight™ RoboColumn Units 200 and 600 µl, Rev A Click to download
6297 Foresight Prepacked Plates and Columns Product Information Sheet Click to download
10024831 Instruction Manual, Foresight™ Chromatography Media Columns 1 and 5 ml, Rev A Click to download
10024413 Instruction Manual, Foresight™ Chromatography Media Filter Plates, Rev A Click to download
D116698 Instruction Manual, Nuvia™ IMAC Resin Click to download
10023853 Instruction Manual, Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin, Rev B Click to download
10033316 Instruction Manual, Nuvia HR-S Strong Cation Exchange Media, Rev A Click to download
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
10014430 Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media, Rev C Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up Click to download [ Add to Cart (Free) ]
6982 Mixed-Mode Resins/Media Selection Guide Click to download [ Add to Cart (Free) ]
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6786 Monoclonal Antibody Purification Resin Selection Card, Ver A Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6791 Virus Purification Resin Selection Card, Ver A Click to download
6792 Aggregate Purification Resin Selection Card, Ver A Click to download

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