Criterion™ XT Tris-Acetate Precast Gels

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Criterion XT Tris-Acetate Precast Gels

Overview

Criterion XT Tris-Acetate Gels

Criterion XT precast gels are formulated at a near-neutral pH, significantly delaying acrylamide hydrolysis compared to traditional Laemmli systems. Their chemical composition allows maximum stability.
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Description

Criterion™ XT gels are ideal for vertical midi protein gel electrophoresis, are designed to work with optimized sample and running buffers, and have an extended shelf life. Like traditional Laemmli systems, Criterion XT precast gels use discontinuous buffer ion fronts that form moving boundaries to stack and then separate proteins. Criterion XT Tris-acetate gels are formulated using a Tris-acetate buffer system (pH 7.0) that, when run with XT Tricine running buffer, separates large denatured proteins by molecular weight.

Features and Benefits

  • 8 month shelf life
  • Made without SDS, so they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
  • Nonreducing and nondenaturing native conditions of native PAGE preserve biological activity and can improve epitope detection by antibodies
  • Neutral pH significantly delays acrylamide hydrolysis as compared to traditional Laemmli systems
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Screen new samples
  • Evaluate sample preparation conditions

To further refine the resolution range, refer to the Criterion XT Tris-Acetate Gel chart.


Specifications

Gel dimensions (W x L x thickness), cm 13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm 15.0 x 10.6 x .53
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Gel storage conditions Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature* 8 months
  Buffer volume, ml Upper, 60; lower, 400
Recommended sample buffer XT sample buffer + XT reducing agent
Recommended running buffer XT Tricine running buffer
  Gel chemistry Tris-acetate
  Gel format Midi
* From date of manufacture.  

Ordering

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3–8% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl

3450129
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$16.00 
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3–8% Criterion™ XT Tris-Acetate Protein Gel, 18 well, 30 µl

3450130
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$16.00 
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3–8% Criterion™ XT Tris-Acetate Protein Gel, 26 well, 15 µl

3450131
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$16.00 
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(Discontinued) 3–8% Criterion™ XT Tris-Acetate Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450133
Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) 7% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl

3450135
Discontinued item
Replaced with 3-8% Criterion XT Tris-Acetate Protein Gel, 12+2 well, 45 µl (3450129)

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) 7% Criterion™ XT Tris-Acetate Protein Gel, 18 well, 30 µl

3450136
Discontinued item
Replaced with 3-8% Criterion XT Tris-Acetate Protein Gel, 18-well, 30 µl (3450130)

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) 7% Criterion™ XT Tris-Acetate Protein Gel, 26 well, 15 µl

3450137
Discontinued item
Replaced with 3-8% Criterion XT Tris-Acetate Protein Gel, 26-well, 15 µl (3450131)

This product is no longer available. Please contact technical support for more information on replacement product.

Accessories

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Mini-PROTEAN® Cassette Opening Lever

4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

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$5.00 
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Kits & Reagents

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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$32.00 
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Native Sample Buffer for Protein Gels, 30 ml

1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue

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$16.00 
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$108.00 
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XT Tricine Running Buffer

1610790
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Tris-Acetate Gels

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$72.00 
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XT Sample Buffer

1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels

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$13.00 
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XT Reducing Agent

1610792
1 ml, 20x reducing agent for use with all Criterion XT gels

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$27.00 
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XT Tricine Buffer Kit

1610797
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent

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$110.00 
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Documents

Number Description Options
2788 Criterion Blotting Tray Insert, Rev A Click to download
2912 Criterion XT Precast Gel Flier, Rev A Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
2911 Criterion XT Precast Gels Product Information Sheet, Rev B Click to download
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download
4110130 Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B Click to download

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