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Overview
Description
Description
Criterion™ XT gels are ideal for vertical midi protein gel electrophoresis, are designed to work with optimized sample and running buffers, and have an extended shelf life. Like traditional Laemmli systems, Criterion XT precast gels use discontinuous buffer ion fronts that form moving boundaries to stack and then separate proteins. Criterion XT Tris-acetate gels are formulated using a Tris-acetate buffer system (pH 7.0) that, when run with XT Tricine running buffer, separates large denatured proteins by molecular weight.
Features and Benefits
- 8 month shelf life
- Made without SDS, so they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
- Nonreducing and nondenaturing native conditions of native PAGE preserve biological activity and can improve epitope detection by antibodies
- Neutral pH significantly delays acrylamide hydrolysis as compared to traditional Laemmli systems
- Run 2-D gels in less than a day
Applications and Uses
- Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
- 2-D gel electrophoresis
- Screen new samples
- Evaluate sample preparation conditions
To further refine the resolution range, refer to the Criterion XT Tris-Acetate Gel chart.
Specifications
Specifications
Gel dimensions (W x L x thickness), cm
13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm
15.0 x 10.6 x .53
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
Store flat at 2–8°C; do not freeze
Gel shelf life at recommended temperature*
8 months
Buffer volume, ml
Upper, 60; lower, 400
Recommended sample buffer
XT sample buffer + XT reducing agent
Recommended running buffer
XT Tricine running buffer
Gel chemistry
Tris-acetate
* From date of manufacture.
Ordering
Ordering
items
Use the filters below to refine results!
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3450129
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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(Discontinued) 3–8% Criterion™ XT Tris-Acetate Protein Gel, 18 well, 30 µl
3450130
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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(Discontinued) 3–8% Criterion™ XT Tris-Acetate Protein Gel, 26 well, 15 µl
3450131
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Accessories
Accessories
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(Discontinued) Mini-PROTEAN® Cassette Opening Lever
4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes
Kits & Reagents
Kits & Reagents
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(Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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(Discontinued) Native Sample Buffer for Protein Gels, 30 ml
1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
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(Discontinued) XT Tricine Running Buffer
1610790
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Tris-Acetate Gels
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(Discontinued) XT Sample Buffer
1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels
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(Discontinued) XT Reducing Agent
1610792
1 ml, 20x reducing agent for use with all Criterion XT gels
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(Discontinued) XT Tricine Buffer Kit
1610797
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent
Documents
Documents
Login Required Number Description Options
2788
Criterion Blotting Tray Insert, Rev A
2912
Criterion XT Precast Gel Flier, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C
2911
Criterion XT Precast Gels Product Information Sheet, Rev B
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
4110130
Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B
