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      Criterion™ Tris-HCl Precast Gels

      lsr_criterion_tris_hcl_precast_gels.jpg

      Overview

      Bio-Rad Tris-HCl gels are made without SDS. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.
      Read full description Ordering Information

      Description

      Description

      Criterion Tris-HCL Precast Gels are ideal for vertical midi gel electrophoresis. They provide Laemmli separation patterns using a standard Tris-glycine running buffer system. Criterion gels are compatible with the Criterion cell (1–2 gels) and the Criterion™ Dodeca™ cell (1–12 gels).

      Features and Benefits

      • Reproducible, high-resolution results
      • Fast setup, loading, and run times
      • Numbered, marked well outlines on the cassette ease sample loading and identification
      • Made without SDS, they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
      • Use same buffers and band patterns as Laemmli systems
      • Robust system allows separation of complex samples
      • Run 2-D gels in less than a day

      Applications and Uses

      • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
      • 2-D gel electrophoresis
      • Accurate molecular weight estimation

      Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.

      Related Products

      Specifications

      Specifications

      Gel dimensions (W x L x thickness), cm
      13.3 x 8.7 x 0.1
      Cassette dimensions (W x L x thickness), cm
      15.0 x 10.6 x .53
      Cassette material
      Styrene copolymer
        Comb material
      Polycarbonate
      Storage tray material
      PET
      Gel storage conditions
      Store flat at 2–8°C; do not freeze
        Gel shelf life at recommended temperature*
      12 weeks
        Buffer volume, ml
      Upper, 60; lower, 400
      Recommended sample buffer (Laemmli, dilute 1:1 with sample)
      62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
      Recommended running buffer (Tris/glycine/SDS)
      25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
        Gel chemistry
      Tris-HCl
      * From date of manufacture.
       

      Ordering

      Ordering

      items

      Use the filters below to refine results!

      345-0009_tn.jpg
      (Discontinued) 10% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
      3450009
      Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0010_tn.jpg
      (Discontinued) 10% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
      3450010
      Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0011_tn.jpg
      (Discontinued) 10% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
      3450011
      Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-9949_tn.jpg
      (Discontinued) 10.5–14% Criterion™ Tris-HCl Gel, 12+2 well, 45 µl
      3459949
      Pkg of 1, 10.5–14% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
      345-0042_tn.jpg
      (Discontinued) 10–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
      3450042
      Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0043_tn.jpg
      (Discontinued) 10–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
      3450043
      Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0044_tn.jpg
      (Discontinued) 10–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
      3450044
      Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0015_tn.jpg
      (Discontinued) 12.5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
      3450015
      Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0014_tn.jpg
      (Discontinued) 12.5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
      3450014
      Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0016_tn.jpg
      (Discontinued) 12.5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
      3450016
      Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0019_tn.jpg
      (Discontinued) 15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
      3450019
      Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0020_tn.jpg
      (Discontinued) 15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
      3450020
      Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0021_tn.jpg
      (Discontinued) 15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
      3450021
      Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0027_tn.jpg
      (Discontinued) 4–15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
      3450027
      Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0028_tn.jpg
      (Discontinued) 4–15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
      3450028
      Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0029_tn.jpg
      (Discontinued) 4–15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
      3450029
      Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0032_tn.jpg
      (Discontinued) 4–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl
      3450032
      Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0033_tn.jpg
      (Discontinued) 4–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl
      3450033
      Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0034_tn.jpg
      (Discontinued) 4–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl
      3450034
      Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0101_tn.jpg
      (Discontinued) 10% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
      3450101
      Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0102_tn.jpg
      (Discontinued) 12.5% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
      3450102
      Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0104_tn.jpg
      (Discontinued) 4–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
      3450104
      Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
      345-0107_tn.jpg
      (Discontinued) 10–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl
      3450107
      Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

      Accessories

      Accessories

      Kits & Reagents

      Kits & Reagents

      161-0732_tn.jpg
      (Discontinued) 10x Tris/Glycine/SDS
      1610732
      Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
      161-0734_tn.jpg
      (Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
      1610734
      Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
      161-0744_tn.jpg
      (Discontinued) 10x Tris/Tricine/SDS Running Buffer, 1 L
      1610744
      Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
      161-0771_tn.jpg
      (Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
      1610771
      Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
      161-0772_tn.jpg
      (Discontinued) 10x Tris/Glycine/SDS
      1610772
      Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
      161-0738_tn.jpg
      (Discontinued) Native Sample Buffer for Protein Gels, 30 ml
      1610738
      30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue

      Documents

      Documents

      Number Description Options
      0512
      Evaluation of Stain-Free Gels for Gel-MS Applications (Poster)
      6040
      Electrophoresis Guide, Interactive PDF, Rev C
      2788
      Criterion Blotting Tray Insert, Rev A
      2710
      Criterion™ Precast Gel System Brochure, Rev C
      5981
      Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart
      6147
      Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B
      4110001
      Instruction Manual, Criterion™ Gel Application Guide, Ver H