Criterion™ Tris-HCl Precast Gels

Image
lsr_criterion_tris_hcl_precast_gels.jpg

Overview

Bio-Rad Tris-HCl gels are made without SDS. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.
Read full description Ordering Information

Description

Description

Criterion Tris-HCL Precast Gels are ideal for vertical midi gel electrophoresis. They provide Laemmli separation patterns using a standard Tris-glycine running buffer system. Criterion gels are compatible with the Criterion cell (1–2 gels) and the Criterion™ Dodeca™ cell (1–12 gels).

Features and Benefits

  • Reproducible, high-resolution results
  • Fast setup, loading, and run times
  • Numbered, marked well outlines on the cassette ease sample loading and identification
  • Made without SDS, they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
  • Use same buffers and band patterns as Laemmli systems
  • Robust system allows separation of complex samples
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Accurate molecular weight estimation

Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.

Specifications

Specifications

Gel dimensions (W x L x thickness), cm
13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm
15.0 x 10.6 x .53
Cassette material
Styrene copolymer
  Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature*
12 weeks
  Buffer volume, ml
Upper, 60; lower, 400
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
  Gel chemistry
Tris-HCl
* From date of manufacture.
 

Ordering

Ordering

items

Use the filters below to refine results!

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3450009
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0010_tn.jpg
3450010
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0011_tn.jpg
3450011
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-9949_tn.jpg
3459949
Pkg of 1, 10.5–14% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
345-0042_tn.jpg
3450042
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0043_tn.jpg
3450043
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0044_tn.jpg
3450044
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0015_tn.jpg
3450015
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0014_tn.jpg
3450014
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0016_tn.jpg
3450016
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0017_tn.jpg
3450017
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0019_tn.jpg
3450019
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0020_tn.jpg
3450020
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0021_tn.jpg
3450021
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0027_tn.jpg
3450027
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0028_tn.jpg
3450028
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0029_tn.jpg
3450029
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0032_tn.jpg
3450032
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0033_tn.jpg
3450033
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0034_tn.jpg
3450034
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0101_tn.jpg
3450101
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0102_tn.jpg
3450102
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0104_tn.jpg
3450104
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0107_tn.jpg
3450107
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
345-0001_tn.jpg
3450001
Discontinued item
No direct replacement
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3450002
Discontinued item
No direct replacement
345-0003_tn.jpg
3450003
Discontinued item
No direct replacement
345-0005_tn.jpg
3450005
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Precast Midi Protein Gel, 12+2 well, 45 µl ()
345-0006_tn.jpg
3450006
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671024)
345-0007_tn.jpg
3450007
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671025)
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3450008
Discontinued item
No direct replacement
345-0012_tn.jpg
3450012
Discontinued item
No direct replacement
345-0022_tn.jpg
3450022
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671122)
345-0023_tn.jpg
3450023
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl (5671123)
345-0024_tn.jpg
3450024
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671124)
345-0025_tn.jpg
3450025
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671125)
345-0026_tn.jpg
3450026
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671122)
345-0030_tn.jpg
3450030
Discontinued item
Replaced with 4–15% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671082)
345-0035_tn.jpg
3450035
Discontinued item
Replaced with 4–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671092)
345-0037_tn.jpg
3450037
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl (5671103)
345-0038_tn.jpg
3450038
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671104)
345-0039_tn.jpg
3450039
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671105)
345-0040_tn.jpg
3450040
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671102)
345-0045_tn.jpg
3450045
Discontinued item
Replaced with 10–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671112)
345-0103_tn.jpg
3450103
Discontinued item
Replaced with 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671081)
345-0105_tn.jpg
3450105
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671101)
345-0106_tn.jpg
3450106
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Gel 11 cm IPG/prep+1 550 µl (5671121)
345-9950_tn.jpg
3459950
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671124)
345-9951_tn.jpg
3459951
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671125)

Accessories

Accessories

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4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

Kits & Reagents

Kits & Reagents

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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
161-0744_tn.jpg
1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
161-0772_tn.jpg
(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
161-0737_tn.jpg
1610737
30 ml, premixed protein sample buffer for SDS-PAGE
161-0738_tn.jpg
1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
161-0610_tn.jpg
(Discontinued) Dithiothreitol (DTT)
1610610
1 g, dithiothreitol (DTT) reducing agent
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(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent
161-0710_tn.jpg
(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

Documents

Documents

Number Description Options
0512
Evaluation of Stain-Free Gels for Gel-MS Applications (Poster)
6040
Electrophoresis Guide, Interactive PDF, Rev C
2788
Criterion Blotting Tray Insert, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C
5981
Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart
6147
Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H