Criterion™ Tris-HCl Precast Gels

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Criterion Tris-HCl Precast Gels

Overview

Criterion Tris-HCI Gels

Bio-Rad Tris-HCl gels are made without SDS. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.
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Ordering Information


Description

Criterion Tris-HCL Precast Gels are ideal for vertical midi gel electrophoresis. They provide Laemmli separation patterns using a standard Tris-glycine running buffer system. Criterion gels are compatible with the Criterion cell (1–2 gels) and the Criterion™ Dodeca™ cell (1–12 gels).

Features and Benefits

  • Reproducible, high-resolution results
  • Fast setup, loading, and run times
  • Numbered, marked well outlines on the cassette ease sample loading and identification
  • Made without SDS, they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
  • Use same buffers and band patterns as Laemmli systems
  • Robust system allows separation of complex samples
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Accurate molecular weight estimation

Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.


Specifications

Gel dimensions (W x L x thickness), cm 13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm 15.0 x 10.6 x .53
Cassette material Styrene copolymer
  Comb material Polycarbonate
Storage tray material PET
Gel storage conditions Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature* 12 weeks
  Buffer volume, ml Upper, 60; lower, 400
Recommended sample buffer (Laemmli, dilute 1:1 with sample) 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/Glycine/SDS) 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
  Gel chemistry Tris-HCl
  Gel format Midi
* From date of manufacture.  

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10% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450009
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$20.00 
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10% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450010
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450011
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10.5–14% Criterion™ Tris-HCl Gel, 12+2 well, 45 µl

3459949
Pkg of 1, 10.5–14% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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10–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450042
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450043
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450044
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450015
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450014
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450016
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450017
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

This product is being discontinued soon. Please contact customer service for more information.
15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450019
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$20.00 
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15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450020
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450021
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450027
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–15% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450028
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–15% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450029
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–20% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450032
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–20% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450033
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–20% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450034
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450101
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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12.5% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450102
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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4–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450104
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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10–20% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450107
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450001
Discontinued item
No direct replacement

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450002
Discontinued item
No direct replacement

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) 5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450003
Discontinued item
No direct replacement

This product is no longer available. Please contact technical support for more information on replacement product.
7.5% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450005
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Precast Midi Protein Gel, 12+2 well, 45 µl ()

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 7.5% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450006
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671024)

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) 7.5% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450007
Discontinued item
Replaced with 7.5% Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671025)

This product is no longer available. Please contact technical support for more information on replacement product.
7.5% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450008
Discontinued item
No direct replacement

This product is being discontinued soon. Please contact customer service for more information.
10% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450012
Discontinued item
No direct replacement

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 15% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450022
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671122)

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) 18% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450023
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl (5671123)

This product is no longer available. Please contact technical support for more information on replacement product.
18% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450024
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671124)

This product is being discontinued soon. Please contact customer service for more information.
18% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450025
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671125)

This product is being discontinued soon. Please contact customer service for more information.
18% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450026
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671122)

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 4–15% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450030
Discontinued item
Replaced with 4–15% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671082)

This product is no longer available. Please contact technical support for more information on replacement product.
4–20% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450035
Discontinued item
Replaced with 4–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671092)

This product is being discontinued soon. Please contact customer service for more information.
8–16% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

3450037
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 12+2 well, 45 µl (5671103)

This product is being discontinued soon. Please contact customer service for more information.
8–16% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3450038
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671104)

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 8–16% Criterion™ Tris-HCl Protein Gel, 26 well, 15 µl

3450039
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671105)

This product is no longer available. Please contact technical support for more information on replacement product.
8–16% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450040
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671102)

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 10–20% Criterion™ Tris-HCl Protein Gel, Prep+2 well, 700 µl

3450045
Discontinued item
Replaced with 10–20% Criterion™ TGX™ Precast Midi Protein Gel, Prep+2 well, 700 µl (5671112)

This product is no longer available. Please contact technical support for more information on replacement product.
4–15% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450103
Discontinued item
Replaced with 4–15% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671081)

This product is being discontinued soon. Please contact customer service for more information.
8–16% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450105
Discontinued item
Replaced with 8–16% Criterion™ TGX™ Precast Midi Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5671101)

This product is being discontinued soon. Please contact customer service for more information.
10.5–14% Criterion™ Tris-HCl Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450106
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Gel 11 cm IPG/prep+1 550 µl (5671121)

This product is being discontinued soon. Please contact customer service for more information.
10.5–14% Criterion™ Tris-HCl Protein Gel, 18 well, 30 µl

3459950
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 18-well, 30 µl (5671124)

This product is being discontinued soon. Please contact customer service for more information.
(Discontinued) 10.5–14% Criterion™ Tris-HCl Gel, 26 well, 15 µl

3459951
Discontinued item
Replaced with Any kD™ Criterion™ TGX™ Precast Midi Protein Gel, 26-well, 15 µl (5671125)

This product is no longer available. Please contact technical support for more information on replacement product.

Accessories

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Mini-PROTEAN® Cassette Opening Lever

4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

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$5.00 
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Kits & Reagents

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10x Tris/Glycine/SDS

1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$32.00 
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$32.00 
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10x Tris/Tricine/SDS Running Buffer, 1 L

1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$100.00 
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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$108.00 
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10x Tris/Glycine/SDS

1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$108.00 
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2x Laemmli Sample Buffer

1610737
30 ml, premixed protein sample buffer for SDS-PAGE

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$14.00 
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Native Sample Buffer for Protein Gels, 30 ml

1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue

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$16.00 
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Dithiothreitol (DTT)

1610610
1 g, dithiothreitol (DTT) reducing agent

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$31.00 
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Dithiothreitol (DTT)

1610611
5 g, dithiothreitol (DTT) reducing agent

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$94.00 
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2-Mercaptoethanol

1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

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$27.00 
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Hazardous  

Documents

Number Description Options
0512 Evaluation of Stain-Free Gels for Gel-MS Applications (Poster) Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev C Click to download
2788 Criterion Blotting Tray Insert, Rev A Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
5981 Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart Click to download
6147 Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B Click to download
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download

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