Criterion TGX Stain-Free Precast Gels

Criterion TGX Stain-Free precast gels are based on the long shelf life TGX formulation and have been developed for rapid fluorescent detection of proteins with the GelDoc Go and ChemiDoc MP imagers.

Ordering Information

Overview

The new Criterion TGX (Tris-Glycine eXtended) Stain-Free™ precast gels for PAGE are based on the long shelf life TGX formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with the GelDoc Go and ChemiDoc™ MP imaging systems.*

Benefits include:

Fast run times with protein separation, gel imaging, and complete analysis in less than 30 min
Rapid fluorescent detection with the GelDoc Go and ChemiDoc MP imaging systems
Comparable sensitivity to Coomassie stain
Better reproducibility and quantitation compared to other staining procedures
Inexpensive Laemmli buffer system, low running costs
Protein transfer in as little as 15 min
Same gel can be used for staining, western blotting, or mass spectrometry

TGX Stain-Free gels retain Laemmli-like separation characteristics using standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min using the Criterion electrophoresis cell and then visualized with the GelDoc Go and ChemiDoc MP imagers in as little as 2.5 min. The trihalo compounds react with tryptophan residues in a UV-induced reaction to produce fluorescence, which can be easily detected by the GelDoc Go and ChemiDoc MP imagers within gels or on a PVDF membrane after transfer.

*These gels can also be imaged on the discontinued Criterion Stain Free™ imaging system, which has been replaced by the GelDoc Go and ChemiDoc MP imaging systems.

Ready to make the switch to Stain-Free enabled precast gels? Use the Western Blot Selector Tool to easily find gels compatible for your next experiment.

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Specifications

Gel dimensions (W x L x thickness)

133 x 87 x 1 mm

Cassette dimensions (W x L x thickness)

150 x 106 x 5.3 mm

Cassette material

Styrene copolymer

Comb material

Polycarbonate

Gel storage conditions

Store flat at 4°C; do not freeze

Shelf life at 2–8°C*

12 months

Recommended sample buffer (Laemmli, dilute 1:1 with sample)

62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue

Recommended running buffer (Tris/glycine/SDS)

25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3

Run times at constant voltage: 200 V

42–50 min

Run times at constant voltage: 300 V

20–26 min

* From date of manufacture.

Ordering

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(Discontinued) 7.5% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl

5678023

Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 7.5% Criterion™ TGX Stain-Free™ Protein Gel, 18 well, 30 µl

5678024

Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 7.5% Criterion™ TGX Stain-Free™ Protein Gel, 26 well, 15 µl

5678025

Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 10% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl

5678033

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 10% Criterion™ TGX Stain-Free™ Protein Gel, 18 well, 30 µl

5678034

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 10% Criterion™ TGX Stain-Free™ Protein Gel, 26 well, 15 µl

5678035

Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 12% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl

5678043

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 12% Criterion™ TGX Stain-Free™ Protein Gel, 18 well, 30 µl

5678044

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 12% Criterion™ TGX Stain-Free™ Protein Gel, 26 well, 15 µl

5678045

Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

(Discontinued) 4–15% Criterion™ TGX Stain-Free™ Protein Gel, 11 cm IPG/prep+1 well, 550 µl

5678081