Criterion TGX Stain-Free Precast Gels

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Overview

Criterion TGX Stain-Free precast gels are based on the long shelf life TGX formulation and have been developed for rapid fluorescent detection of proteins with the Gel Doc EZ and ChemiDoc MP imagers.

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Description

Description

Stain-Free Technology

What Is Stain-Free Technology?

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The new Criterion™ TGX (Tris-Glycine eXtended) Stain-Free™ precast gels for PAGE are based on the long shelf life TGX formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with the Gel Doc™ EZ and ChemiDoc™ MP imaging systems.*

Benefits include:

  • Fast run times with protein separation, gel imaging, and complete analysis in less than 30 min
  • Rapid fluorescent detection with the Gel Doc EZ and ChemiDoc MP imaging systems
  • Comparable sensitivity to Coomassie stain
  • Better reproducibility and quantitation compared to other staining procedures
  • Inexpensive Laemmli buffer system, low running costs
  • Protein transfer in as little as 15 min
  • Same gel can be used for staining, western blotting, or mass spectrometry

TGX Stain-Free gels retain Laemmli-like separation characteristics using standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min using the Criterion electrophoresis cell and then visualized with the Gel Doc EZ and ChemiDoc MP imagers in as little as 2.5 min. The trihalo compounds react with tryptophan residues in a UV-induced reaction to produce fluorescence, which can be easily detected by the Gel Doc EZ and ChemiDoc MP imagers within gels or on a PVDF membrane after transfer.

*These gels can also be imaged on the discontinued Criterion Stain Free™ imaging system, which has been replaced by the Gel Doc EZ and ChemiDoc MP imaging systems.

Specifications

Specifications

Gel dimensions (W x L x thickness)
133 x 87 x 1 mm
Cassette dimensions (W x L x thickness)
150 x 106 x 5.3 mm
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Gel storage conditions
Store flat at 4°C; do not freeze
Shelf life at 2–8°C*
12 months
Recommended sample buffer (Laemmli, dilute 1:1 with sample)
62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue
Recommended running buffer (Tris/glycine/SDS)
25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
Run times at constant voltage: 200 V
42–50 min
Run times at constant voltage: 300 V
20–26 min
* From date of manufacture.
 

Ordering

Ordering

items

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5678023
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678024
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678025
Pkg of 1, 7.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678033
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678034
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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567-8xxx_tn.jpg
5678035
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678043
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678044
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678045
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678071
Discontinued item
Replaced with 4–15% Criterion™ TGX Stain-Free™ Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5678081)
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5678072
Pkg of 1, 18% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678073
Discontinued item
Replaced with 12% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl (5678043)
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5678074
Discontinued item
Replaced with 12% Criterion™ TGX Stain-Free™ Protein Gel, 18-well, 30 µl (5678044)
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5678081

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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5678082

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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5678083

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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5678084

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678085

Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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5678091

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678092

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678093

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678094

Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678095
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678101
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678102
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678103
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678104
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers
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5678105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodecatrade; electrophoresis cells and stain-free enabled imagers
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5678111
Discontinued item
Replaced with Any kD™ Criterion™ TGX Stain-Free™ Protein Gel, 11 cm IPG/prep+1 well, 550 µl (5678121)
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5678112
Discontinued item
Replaced with Any kD™ Criterion™ TGX Stain-Free™ Protein Gel, Prep+2 well, 700 µl (5678122)
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5678113
Discontinued item
Replaced with Any kD™ Criterion™ TGX Stain-Free™ Gel 12+2 well, 45 µl (5678123)
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5678114
Discontinued item
Replaced with Any kD™ Criterion™ TGX Stain-Free™ Gel 18-well, 30 µl (5678124)
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5678115
Discontinued item
Replaced with Any kD™ Criterion™ TGX Stain-Free™ Gel 26-well, 15 µl (5678125)
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5678121

Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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5678122

Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678123

Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678124

Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

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567-8xxx_tn.jpg
5678125

Pkg of 1, Any kD precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers

Accessories

Accessories

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(Discontinued) Criterion™ Cell
1656001
Vertical midi-format electrophoresis cell, includes buffer tank, lid with power cables, 3 sample loading guides (12+2-well, 18-well, and 26-well)
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1654130
Vertical midi-format electrophoresis cell, includes buffer tank with built-in cooling coil, lid with power cables, 12 gel capacity

Kits & Reagents

Kits & Reagents

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1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

Documents

Documents

Number Description Options
6356
Traditional vs. Stain-Free Western Blotting
6427
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6434
Western Blot Normalization Using Image Lab™ Software, Quick Start Guide
[ Add to Cart (Free) ]
6507
TGX and TGX Stain-Free Gels: Publications List
0512
Evaluation of Stain-Free Gels for Gel-MS Applications (Poster)
5974
Mini-PROTEAN® and Criterion™ TGX Stain-Free™ Precast Gels Product Information Sheet, Rev E
6040
Electrophoresis Guide, Interactive PDF, Rev C
5810
Compatibility of the Criterion Stain-Free Gel Imaging System with Mass Spectrometric Protein Analysis
5981
Criterion Tris-HCl to Criterion TGX and TGX Stain-Free Precast Gels Catalog Number Conversion Chart
6008
Comparison of the Criterion TGX Stain-Free Precast Gel System and Standard Coomassie Staining Procedures for Running and Imaging Protein Gels
6166
Multiplex Fluorescent Blot Detection: A Troubleshooting Guide
6390
General Stain-Free Western Blotting Protocol
[ Add to Cart (Free) ]
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H
6147
Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B
6453
Utilize Stain-Free Technology in a Variety of Workflows, Rev B
[ Add to Cart (Free) ]