CM Affi-Gel® Blue Gel

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Overview

CM Affi-Gel Blue Gel contains Cibacron Blue F3GA dye covalently coupled to CM Bio-Gel A. This bifunctional affinity chromatography gel binds both albumin and serum proteases.
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Description

Description

CM Affi-Gel Blue gel contains Cibacron Blue F3GA dye covalently coupled to CM Bio-Gel A. This bifunctional gel binds both albumin and serum proteases. CM Affi-Gel Blue chromatography provides a convenient initial step in the purification of serum proteins. Features of CM Affi-Gel Blue gel include:

  • Rapid removal of ≥90% of albumin and all plasminogen in serum samples
  • No prior sample preparation needed
  • ≥80% yield of stable antiserum, free of albumin and protease activity

 

Specifications

Specifications

Matrix
Crosslinked Agarose
Functional group
Cibacron Blue F3GA and CM
Specificity
Albumin and serum proteins
Capacity
0.17–0.5 ml serum/ml gel
Working pH
2–11
Pressure limit
15 psi (1 bar)
Applications
Produces albumin and protease-free antibody preparation from serum without prior dialysis

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Documents

Documents

Number Description Options
LIT308
Instruction Manual, CM Affi-Gel® Blue Gel, Rev C