Affi-Gel® Boronate Gel

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Overview

Affi-Gel boronate-derivatized polyacrylamide gel has a high binding capacity and is ideal for the affinity purification of molecules with adjacent coplanar cis-hydroxyl groups (cis-diols).
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Description

Description

Affi-Gel boronate-derivatized polyacrylamide gel has affinity for coplanar adjacent cis-hydroxyl groups (cis-diols) and a high binding capacity, which provides highly efficient separation of low molecular weight molecules, such as nucleotides, nucleosides, catecholamines, and sugars. It has a sorbitol capacity of 130 µmol/ml.

Other features include:

  • High boronate capacity — 1.05 ± 0.15 meq/g
  • High diol capacity — binds 130 µmol sorbitol/ml gel
  • Spherical polyacrylamide matrix — excellent flow rate, resolution, and reproducibility
  • Low nonspecific absorption, optimum porosity — low exclusion limit precludes significant binding of high molecular weight molecules, while allowing rapid equilibration and binding of low and medium molecular weight molecules

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1536103
5 g, ready-to-use affinity chromatography support, for small MW molecules (nucleotides, nucleosides, catecholamines, and sugars)
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153-6104
50 g, boronate affinity chromatography media, for small MW molecules (nucleotides, nucleosides, catecholamines, and sugars)

Documents

Documents

Number Description Options
1066
Affi-Gel® Boronate Affinity Gel to Separate Ribonucleotides, Ribonucleosides, Sugars, Catecholamines, and Coenzymes, Rev B