Affi-Gel blue affinity gel is a beaded, crosslinked agarose gel with covalently attached Cibacron Blue F3GA dye. It contains >1.9 mg dye per ml of gel and has a typical albumin capacity of >11 mg/ml. Affi-Gel Blue gel purifies a large range of proteins from widely divergent origins. The blue dye functions as an ionic, hydrophobic, aromatic, or sterically active binding site in various applications.
Affi-Gel Blue is ideally suited for albumin removal (using 50–100 mesh) and enzyme purification (using 100–200 mesh). Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of the eluent buffers.
For more information, see Bulletin 1107.
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