Affi-Gel Blue Gel

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Overview

Affi-Gel Blue Gel is a crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye. The blue dye functions as an ionic, hydrophobic, aromatic, or sterically active binding site.
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Description

Description

Affi-Gel blue affinity gel is a beaded, crosslinked agarose gel with covalently attached Cibacron Blue F3GA dye. It contains >1.9 mg dye per ml of gel and has a typical albumin capacity of >11 mg/ml. Affi-Gel Blue gel purifies a large range of proteins from widely divergent origins. The blue dye functions as an ionic, hydrophobic, aromatic, or sterically active binding site in various applications.

Affi-Gel Blue is ideally suited for albumin removal (using 50–100 mesh) and enzyme purification (using 100–200 mesh). Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of the eluent buffers.

For more information, see Bulletin 1107.

Specifications

Specifications

Functional group
Cibacron blue
Ligand concentration
≥ 1.9 mg/ml
Typical protein capacity
> 11 mg/ml
pH stability
4–11
Pressure limit
15 psi
Flow rate
15–25 cm/hr
Bead size:
   50–100 mesh
   100–200 mesh

150–300 µm
75–150 µm
Storage
4°C
Shipping buffer
0.05 % NaN3 in water
Sanitization
0.1 M NaOH

Ordering

Ordering

items

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(Discontinued) Affi-Gel Blue Gel
1537301
100 ml, 50–100 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye
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(Discontinued) Affi-Gel Blue Gel
1537302
100 ml, 100–200 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye

Kits & Reagents

Kits & Reagents

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7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue
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7324642
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324644
Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7326712
Kit for removal of albumin from serum/plasma samples, includes Micro Bio-Spin columns prepacked with Affi-Gel Blue gel for 2 preps
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7326708
Pkg of 25, Micro Bio-Spin columns prepacked with Affi-Gel Blue gel, 100–200 mesh, for removal of up to 90% albumin from serum/plasma samples

Documents

Documents

Number Description Options
1107
Affi-Gel® Blue Affinity Chromatography Gel for Enzyme and Blood Protein Purifications
4110139
Instruction Manual, Aurum™ Affi-Gel Blue Mini Kits and Columns Sample Preparation, Rev B
LIT590
Instruction Manual, Affi-Gel Blue Gel
3168
Comparison of Cibacron Blue Affinity-Based Formats for Depletion of Albumin From Samples Prior to Two-Dimensional Gel Electrophoresis