Description
Description
This one-step reverse transcription digital PCR supermix delivers improved efficiency, specificity, and sensitivity for precise RNA target quantification with Droplet Digital PCR.
Key Benefits
- Absolute quantification by Droplet Digital PCR in a convenient single-reaction format
- Contains all components required for hydrolysis probe–based RT-ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
- Contains RNase inhibitor to protect the RNA throughout the entire workflow
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Viral load detection
- Pathogen detection
Packaging Options
| Number of 20 µl Reactions | Catalog Number |
|---|---|
| 200 | 1864021 |
| 500 | 1864022 |
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
- Digital PCR consumables
- PrimePCR ddPCR Assays
Additional Equipment
This digital PCR supermix requires the use of one or more of the following instruments and accessories:
- Automated Droplet Generator
- QX200 Droplet Generator
- QX200 Droplet Reader
- PX1 PCR Plate Sealer
- C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module
More Information
This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX200 Droplet Digital PCR System and QX200 AutoDG Droplet Digital System.
Specifications
Specifications
Ordering
Ordering
items
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200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
Consumables
Consumables
70 ml (10 x 7 ml), oil for use with droplet generator in the QX600/QX200 Droplet Digital™ PCR Systems
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 24, cartridges for use with the QX200 Droplet Generator
Pkg of 24, gaskets for use with QX200 Droplet Generator
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200 Droplet Generator
Equipment
Equipment
AutoDG Droplet Digital PCR System for EvaGreen or probe-based digital PCR applications, includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QX Manager Software, Standard Edition.
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
