1-Step RT-ddPCR Advanced Kit for Probes

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Overview

This digital PCR supermix kit enables absolute quantification of target RNA molecules in a one-step format.

Read full description Ordering Information

Description

Description

 

This one-step reverse transcription digital PCR supermix delivers improved efficiency, specificity, and sensitivity for precise RNA target quantification with Droplet Digital PCR.

Key Benefits

  • Absolute quantification by Droplet Digital PCR in a convenient single-reaction format
  • Contains all components required for hydrolysis probe–based RT-ddPCR except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
  • Contains RNase inhibitor to protect the RNA throughout the entire workflow

Applications and Uses

  • Absolute quantification
  • Gene expression analysis
  • Viral load detection
  • Pathogen detection

Packaging Options

Number of 20 µl Reactions Catalog Number
200 1864021
500 1864022

 

Additional Reagents

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of one or more of the following instruments and accessories:

More Information

This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX200 Droplet Digital PCR System and QX200 AutoDG Droplet Digital System.

Specifications

Specifications

Storage at –20°C
Up to 12 months (refer to expiration date)
Template compatibility
RNA

Ordering

Ordering

items

Use the filters below to refine results!

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1864021
200 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems
one-step-rt-ddpcr-advanced-kit-for-probes-1864022-thumb.jpg
1864022
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

Consumables

Consumables

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1863005
70 ml (10 x 7 ml), oil for use with droplet generator in the QX200/QX100™ Droplet Digital™ PCR Systems
qx100-ddpcr-buffer-control-kit-186-3052-thumb.jpg
1863052
9 ml (2 x 4.5 ml), 2x buffer, for use as blank control with Droplet Generation Oil for Probes
qx100-droplet-reader-oil-2l-186-3004-thumb.jpg
1863004
2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems
dg8-cartridges-for-qx100-qx200-droplet-generator-186-4008-thumb.jpg
1864008
Pkg of 24, cartridges for use with the QX200™/QX100™ Droplet Generator
qx100-dg8-gaskets-186-3009-thumb.jpg
1863009
Pkg of 24, gaskets for use with QX200™/QX100™ Droplet Generator
droplet-generator-cartridges-and-gaskets-186-4007-thumb.jpg
1864007
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200™/QX100™ Droplet Generator
pierceable-foil-heat-seal-181-4040-thumb.jpg
1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Equipment

Equipment

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1864100

AutoDG Droplet Digital PCR System for EvaGreen or probe-based digital PCR applications, includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QX Manager Software, Standard Edition.

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1864001
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
automated-droplet-generator-186-4101-thumb.jpg
1864101
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX200/QX100; Droplet Digital PCR Systems
qx200-droplet-generator-186-4002-thumb.jpg
1864002
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
qx200-droplet-reader-186-4003-thumb.jpg
(Discontinued) QX200 Droplet Reader
1864003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
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1851197
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
PX1-PCR-Plate-Sealer-SKU-181-4000-thumb.jpg
(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

Documents

Documents

Number Description Options
10049226
One-Step RT-ddPCR Advanced Kit for Probes Product Insert
10040718
Droplet Generation Oil for Probes Product Insert
6311
QX200 Droplet Digital PCR System Brochure, Rev C
[ Add to Cart (Free) ]
10043138
Instruction Manual, Automated Droplet Generator for Droplet Digital™ PCR Systems
10031907
Instruction Manual, QX200™ Droplet Generator, Rev C
10031906
Instruction Manual, QX200 Droplet Reader and QuantaSoft Software