10x Tris/Glycine Buffer for Western Blots and Native Gels #1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
Available Safety Data Sheets
Safety Data Sheet
Overview
Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.
- For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol
- For tank blotting of native gels, without methanol
- As a running buffer for native gels
Precast gels that can be used for native electrophoresis
| Precast Gel Type | Size | Gel Chemistry |
| Mini-PROTEAN® TGX™ | Mini | Laemmli-like, long shelf life, fast separation with high resolution |
| Criterion™ TGX™ | Midi | |
| Mini-PROTEAN® TGX Stain-Free™ | Mini | Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection |
| Criterion™ TGX Stain-Free™ | Midi | |
| Criterion™ Tris-HCl | Midi | Standard Laemmli |
| Criterion Stain Free™ Tris-HCl | Midi | Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection |
| Criterion XT Tris-Acetate | Midi | Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins |
Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.
Packaging Options
| 10x Buffer Volume | Catalog Number |
| 1 L | 1610734 |
| 5 L cube | 1610771 |
Related Products
- PrecisionAb™ Validated Western Blotting Antibodies
- Native Sample Buffer (1610738)
- Criterion Staining/Blotting Trays with lids (3459921)
- 10x Phosphate Buffered Saline (PBS) (1610780)
- 10x Tris Buffered Saline (TBS) (1706435)
- 1x Phosphate Buffered Saline (PBS) with 1% Casein (1610783)
- 1x Tris Buffered Saline (TBS) with 1% Casein (1610782)
- Blotting-Grade Blocker, nonfat dry milk (1706404)
Related Categories
- Protein Gels
- Western Blotting Membranes and Filter Paper
- Semi-Dry and Rapid Blotting Systems
- Wet/Tank Blotting Systems
- Power Supplies
- Transfer Buffers
- Immunodetection Reagents and Kits
- Gel and Western Imaging Systems
Supporting Documents
- Mini-PROTEAN Precast Gels Instruction Manual and Application Guide
- Criterion Gel Application Guide Instruction Manual
- Mini Trans-Blot® Cell Instruction Manual
- Trans-Blot SD Cell Quick Reference Guide
- Trans-Blot SD Semi-Dry Transfer Cell Instruction Manual