iQ-Check E. coli O157:H7 PCR Detection Kit 

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Overview

Complete sample preparation and PCR kit for the rapid detection of E. coli O157:H7 in food samples. Results available in 12 hr.

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Description

Description

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The iQ-Check E. coli O157:H7 PCR Detection Kit is based on the fast, sensitive, and proven technology of real‑time PCR. It is intended for detecting E. coli O157:H7 in food samples such as raw beef and fresh produce.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 12 hr after enrichment in nonselective medium.

Highly Specific and Reliable

The iQ-Check PCR Test uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions with close strains such as E. coli O55. The test is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check E. coli O157:H7 Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols

The iQ-Check E. coli O157:H7 Kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and it provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Specifications

Specifications

Assay type
PCR
Organism
E. coli O157:H7

Ordering

Ordering

items

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3578114
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
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3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Accessories

Accessories

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TLS0851XTU

Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)

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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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17007992

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

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17007991

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables

Documents

Documents

Number Description Options
10000131517
iQ-Check E. coli O157:H7, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
3311
CFX Opus 96 Real-Time PCR System brochure
3309
CFX Opus 96 and CFX Opus Deepwell Real-Time PCR system product information sheet
7439
Performance Summary, iQ-Check E. coli O157:H7
7160
iQ-Check E. coli O157:H7 Kit Quick Guide: Easy II Extraction Deep Well Protocol Using the Thermoshaker
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
6960
iQ-Check Prep Solution Brochure
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
FSD_17933
Food Safety and Quality Reference Guide
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)
3276
CFX Manager Software (IDE) iQ-Check Prep sample setup Protocol, Quick Guide