iQ-Check E. coli O157:H7 PCR Detection Kit 

iQ-Check E. coli O157:H7 PCR Detection Kit 

Complete sample preparation and PCR kit for the rapid detection of E. coli O157:H7 in food samples. Results available in 12 hr.

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Overview

Consult with a Specialist

The iQ-Check E. coli O157:H7 PCR Detection Kit is based on the fast, sensitive, and proven technology of real‑time PCR. It is intended for detecting E. coli O157:H7 in food samples such as raw beef and fresh produce.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 12 hr after enrichment in nonselective medium.

Highly Specific and Reliable

The iQ-Check PCR Test uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions with close strains such as E. coli O55. The test is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check E. coli O157:H7 Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols

The iQ-Check E. coli O157:H7 Kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and it provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Specifications

Assay type
PCR
Organism
E. coli O157:H7

Ordering

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3578114
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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3554179

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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3564684

Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Accessories

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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design
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17010688
17010688

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

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17010624
2112036XTU

Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas

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17010692
2112021XTU

Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only

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17010691
2112006XTU

Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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17007992

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

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17007991

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables

Documents

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