iQ-Check E. coli O157:H7 PCR Detection Kit

Complete sample preparation and PCR kit for the rapid detection of E. coli O157:H7 in food samples. Results available in 12 hr.

Ordering Information

Overview

Consult with a Specialist

The iQ-Check E. coli O157:H7 PCR Detection Kit is based on the fast, sensitive, and proven technology of real‑time PCR. It is intended for detecting E. coli O157:H7 in food samples such as raw beef and fresh produce.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 12 hr after enrichment in nonselective medium.

Highly Specific and Reliable

The iQ-Check PCR Test uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions with close strains such as E. coli O55. The test is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check E. coli O157:H7 Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols

The iQ-Check E. coli O157:H7 Kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and it provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

Lysis reagent for DNA extraction
Fluorescent probes
PCR amplification mix
Positive and negative PCR controls

Related Products

RAPID'E.coli O157:H7 Medium

Specifications

Assay type

PCR

Organism

E. coli O157:H7

Ordering

items

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(Discontinued) iQ-Check E.coli O157:H7 PCR Detection Kit

3578114

Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

(Discontinued) iQ-Check Free DNA Removal Solution

3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

(Discontinued) iQ-Check Purification Reagent

12012383

Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests

(Discontinued) Buffered Peptone Water Plus

3554179

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Plus

3555790

Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Plus

3555795

Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Plus

3564684

Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Plus

3564686

Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

(Discontinued) Buffered Peptone Water Standard

12013260

Pkg of 5 L x 2 bags — for standard enrichment conditions

(Discontinued) Buffered Peptone Water Standard, dehydrated

12013259

Pkg of 1, 500 g — for standard enrichment conditions

(Discontinued) Buffered Peptone Water Standard, dehydrated

12013258

Pkg of 1, 5 kg — for standard enrichment conditions

Accessories

(Discontinued) Pre-Pierced Plate Sealing Film

3600040

Pkg of 100, PCR plate sealing film, pre-pierced

(Discontinued) PCR Tube 8-Cap Strips, optical, flat, 8 caps

TCS0803XTU

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)

(Discontinued) Multiplate™ 48-Well PCR Plates, low-profile, unskirted, white

MLL4851XTU

Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples

(Discontinued) Multiplate 96-Well PCR Plates, low-profile, unskirted

MLL9651XTU

Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

(Discontinued) Strip Cap Tool for PCR Plates and PCR Tubes

ECT2000

Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes

(Discontinued) Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655XTU

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

(Discontinued) Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white, barcoded

HSP9955XTU

Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

(Discontinued) iQ-Check Deep Well Microplates

3594900

Pkg of 50, 96-well plates plates, deep well

(Discontinued) X-Pierce Films

3593977

Pkg of 100, PCR plate sealing film, pre-pierced

(Discontinued) Microseal 'B' PCR Plate Sealing Film, adhesive, optical

17010701

Pkg of 100, optically clear seal for PCR plates for real-time PCR

(Discontinued) 1.5 ml Conical Tubes, screwcap, sterile

17010678

Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style D), 20–200 µl

17010688

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style H), 100–1,000 µl

2112036XTU

Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style E), 100–1,000 µl

2112021XTU

Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only

(Discontinued) Xcluda Aerosol Barrier Pipet Tips (Style B), 2–20 µl

2112006XTU

Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Equipment

(Discontinued) CFX96 Touch Deep Well Real-Time PCR Detection System

3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.