iQ-Check E. coli O157:H7 PCR Detection Kit 

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iQ-Check <em>E.coli</em> O157:H7

Overview

Key Benefits

Faster Results

  • Test results within hours after 8 hr enrichment in BPW
  • Rapid screening of up to 94 samples

Easy To Use

  • No IMS step
  • Simple DNA extraction
  • Ready-to-use reagents, including for DNA extraction

Minimal Risk

  • No cross-reactions with E.coli O55
  • Screening with no isolated colonies
  • Positive and negative PCR controls, plus an internal inhibition control

A Complete Solution

  • Standardized Bio-Rad reagents:
    • Enrichment media
    • iQ-Check kits
    • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis



Protocol
Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


Open in new window
 
 


Open in new window
Open in new window
Open in new window


Open in new window
 
 


Open in new window
Open in new window
 

Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Complete sample preparation and PCR kit for the rapid detection of E. coli O157:H7 in food samples. Results available in 12 hr.


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Ordering Information


  • Description
  • Specifications
  • Ordering
  • Accessories
  • Equipment
  • Documents

Description

Talk with a Specialist

The iQ-Check E. coli O157:H7 PCR Detection Kit is based on the fast, sensitive, and proven technology of real‑time PCR. It is intended for detecting E. coli O157:H7 in food samples such as raw beef and fresh produce.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 12 hr after enrichment in nonselective medium.

Highly Specific and Reliable

The iQ-Check PCR Test uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions with close strains such as E. coli O55. The test is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check E. coli O157:H7 Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols

The iQ-Check E. coli O157:H7 Kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and it provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Specifications

Assay type PCR
Organism E. coli O157:H7

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iQ-Check E.coli O157:H7 PCR Detection Kit

3578114
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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iQ-Check Free DNA Removal Solution

3594970
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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$570.00 
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iQ-Check Purification Reagent

12012383
Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests

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$103.00 
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STEC Enrichment Broth, dehydrated

3564001
Pkg of 1, 500 g of dehydrated broth

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$103.00 
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STEC Enrichment Broth, dehydrated

3564002
Pkg of 1, 5 kg of dehydrated broth

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$960.00 
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Buffered Peptone Water Plus

3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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$28.00 
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Buffered Peptone Water Plus

3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

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$113.00 
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Buffered Peptone Water Plus

3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

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$114.00 
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Buffered Peptone Water Plus

3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

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$78.00 
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Buffered Peptone Water Plus

3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

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$716.00 
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Buffered Peptone Water Standard

12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions

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$99.00 
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Buffered Peptone Water Standard, dehydrated

12013259
Pkg of 1, 500 g — for standard enrichment conditions

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$68.00 
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Buffered Peptone Water Standard, dehydrated

12013258
Pkg of 1, 5 kg — for standard enrichment conditions

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$622.00 
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Accessories

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list price. If the problem persists, please call customer service at or send an
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Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001XTU
Pkg of 100, optically clear seal for PCR plates for real-time PCR

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Pre-Pierced Plate Sealing Film

3600040
Pkg of 100, PCR plate sealing film, pre-pierced

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$201.00 
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1.5 ml Conical Tubes, screwcap, sterile

2240110XTU
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

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$157.00 
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PCR Tube Strips without Caps, low-profile, 0.2 ml, 8-tube strips

TLS0851XTU
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)

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$130.00 
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PCR Tube 8-Cap Strips, optical, flat, 8 caps

TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)

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$43.00 
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Multiplate™ 48-Well PCR Plates, low-profile, unskirted, white

MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples

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$163.00 
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Multiplate 96-Well PCR Plates, low-profile, unskirted

MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

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$141.00 
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Strip Cap Tool for PCR Plates and PCR Tubes

ECT2000
Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes

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$46.00 
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Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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$298.00 
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Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white, barcoded

HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

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$304.00 
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iQ-Check Deep Well Microplates

3594900
Pkg of 50, 96-well plates plates, deep well

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$285.00 
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Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001
Pkg of 100, optically clear seal for PCR plates

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$191.00 
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X-Pierce Films

3593977
Pkg of 100, PCR plate sealing film, pre-pierced

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$189.00 
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Equipment

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list price. If the problem persists, please call customer service at or send an
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CFX96 Touch Deep Well Real-Time PCR Detection System

3600037
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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Digital Disruptor Genie, Cell Disruptor

3591456
Pkg of 1, cell disruption device, for DNA extraction for iQ-Check® PCR test Detection Kits

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$1,434.00 
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iQ-Check Prep System

3594911
Automated system for DNA extraction and real-time PCR plate set-up, 1 unit

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Heating Thermoshaker

3594995
Pkg of 1, temperature-controlled shaker for cell lysis, for use with iQ-Check PCR Detection Kits

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Deep Well Block for Thermoshaker

3594996
Pkg of 1, deep well block for thermoshaker

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CFX Manager Software, Industrial Diagnostic Edition (IDE)

3593893
1 unit

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$3,178.00 
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Documents

Number Description Options
10000131517 iQ-Check E. coli O157:H7, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
FSD_16439 iQ-Check Real-Time PCR Solution Brochure Click to download
7439 Performance Summary, iQ-Check E. coli O157:H7 Click to download
7160 iQ-Check E. coli O157:H7 Kit Quick Guide: Easy II Extraction Deep Well Protocol Using the Thermoshaker Click to download
6960 iQ-Check Prep Solution Brochure Click to download
10000058391 iQ-Check Free DNA Removal Solution Product Insert Click to download
7322 iQ-Check Free DNA Removal Solution — Quick Guide Click to download
6834 iQ-Check Free DNA Removal Solution Brochure Click to download
10000119734 iQ-Check Purification Reagent Technical sheet Click to download
FSD_17933 Food Safety and Quality Reference Guide Click to download
PF_071 iQ-Check® Quick Guide: Simultaneous Testing for E. coli O157:H7 and Salmonella spp. Rev A Click to download
7274 Complete Solutions for Service Laboratories Click to download
10000128740 Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
10000128738 Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download

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