RAPID'E.coli O157:H7 Medium

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RAPID'<em>E.coli</em> O157:H7 Medium

Overview


Key Benefits

KEY BENEFITS

  • Specific to E.coli O157 serotype H7
  • Only 1 plate required per sample
  • Complete results in 48 hr

Protocol
Detection of Escherichia coli O157:H7
Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


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Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Medium for the direct presumptive identification of Escherichia coli O157:H7.
Read full description

Ordering Information


Description

Talk with a Specialist

RAPID'E.coli O157:H7 Medium is a selective chromogenic agar for the detection, isolation, and presumptive identification of Escherichia coli O157:H7 in human food products and environmental samples.

Key Benefits

  • High specificity to E. coli O157 serotype H7
  • Only 1 plate required per sample
  • Results in 48 hr
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Simple Protocol

RAPID'E.coli O157:H7 Medium provides laboratories with a rapid and cost-effective solution for E. coli O157:H7 detection, given that a single plate is required for each sample. The latex test for confirmation has been validated for use on isolated colonies grown on RAPID'E.coli plates.

Other Applications of RAPID'E.coli O157:H7 Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 16654 reference method)
  • To confirm positive results obtained with the iQ-Check E. coli O157:H7 PCR Detection Kit

Easy-to-Read Results

RAPID'E.coli O157:H7 medium permits the detection of E. coli O157 serotype H7 using specific chromogenic substrates and biochemical activities.

  • Typical E. coli O157:H7 (Sorbitol/SORB +, β-Glucuronidase/GLUC +) colonies are characteristically convex, 1–2 mm in diameter, and dark blue to black in color
  • Atypical GLUC + E. coli O157:H7 are detected and present as dark blue to black colonies
  • Atypical SORB + E. coli O157:H7 are detected and present as blue to turquoise blue colonies

RAPID'E.coli O157:H7 Medium is very specific. Most other bacteria and yeasts are inhibited or produce a different color. The selectivity of RAPID'E.coli O157:H7 Medium can be improved by adding selective agents such as novobiocin and potassium tellurite.

RAPID'E.coli O157:H7 Chromogenic Assay

Enzymatic Activity
E. coli O157:H7
Other E. coli other bacteriaOther bacteria
Glucuronidase - + -
Galactosidase + + -
Sorbitol - + +/-

Ordering Information

RAPID'E.coli O157:H7 Medium is available as a dehydrated agar broth (3564748).


Specifications

Assay type Chromogenic media
Organism E. coli O157:H7

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RAPID'E. coli O157:H7, dehydrated

3564748
Pkg of 1, 100 g of dehydrated agar broth, enough for 1.25 L of medium

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$326.00 
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STEC Enrichment Broth, dehydrated

3564001
Pkg of 1, 500 g of dehydrated broth

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$103.00 
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Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready to use

3555426
Pkg of 6, 225 ml bottles, contains novobiocin

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$62.00 
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Modified Tryptic Soy Broth (mTSB) Base, dehydrated

3564426
Pkg of 1, 500 g of dehydrated base

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$102.00 
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iQ-Check E.coli O157:H7 PCR Detection Kit

3578114
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Documents

Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure Click to download
FSD_17933 Food Safety and Quality Reference Guide Click to download
Bulletin_7244_en RAPID'E.coli O157:H7 Agar Technical Sheet Click to download
Bulletin_7244_fr RAPID'E. coli O157:H7/Gélose Fiche Technique Click to download
7274 Complete Solutions for Service Laboratories Click to download

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