RAPID'E.coli O157:H7 Medium

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Overview

Medium for the direct presumptive identification of Escherichia coli O157:H7.
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Description

Description

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RAPID'E.coli O157:H7 Medium is a selective chromogenic agar for the detection, isolation, and presumptive identification of Escherichia coli O157:H7 in human food products and environmental samples.

Key Benefits

  • High specificity to E. coli O157 serotype H7
  • Only 1 plate required per sample
  • Results in 48 hr
  • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

Simple Protocol

RAPID'E.coli O157:H7 Medium provides laboratories with a rapid and cost-effective solution for E. coli O157:H7 detection, given that a single plate is required for each sample. The latex test for confirmation has been validated for use on isolated colonies grown on RAPID'E.coli plates.

Other Applications of RAPID'E.coli O157:H7 Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 16654 reference method)
  • To confirm positive results obtained with the iQ-Check E. coli O157:H7 PCR Detection Kit

Easy-to-Read Results

RAPID'E.coli O157:H7 medium permits the detection of E. coli O157 serotype H7 using specific chromogenic substrates and biochemical activities.

  • Typical E. coli O157:H7 (Sorbitol/SORB +, β-Glucuronidase/GLUC +) colonies are characteristically convex, 1–2 mm in diameter, and dark blue to black in color
  • Atypical GLUC + E. coli O157:H7 are detected and present as dark blue to black colonies
  • Atypical SORB + E. coli O157:H7 are detected and present as blue to turquoise blue colonies

RAPID'E.coli O157:H7 Medium is very specific. Most other bacteria and yeasts are inhibited or produce a different color. The selectivity of RAPID'E.coli O157:H7 Medium can be improved by adding selective agents such as novobiocin and potassium tellurite.

RAPID'E.coli O157:H7 Chromogenic Assay

Enzymatic Activity
E. coli O157:H7
Other E. coli other bacteriaOther bacteria
Glucuronidase - + -
Galactosidase + + -
Sorbitol - + +/-

Ordering Information

RAPID'E.coli O157:H7 Medium is available as a dehydrated agar broth (3564748).

Specifications

Specifications

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3564748
Pkg of 1, 100 g of dehydrated agar broth, enough for 1.25 L of medium
3564001
Pkg of 1, 500 g of dehydrated broth
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3555426
Pkg of 6, 225 ml bottles, contains novobiocin
356-4426_160.jpg
3564426
Pkg of 1, 500 g of dehydrated base
iQ-Check_Listeria_spp _2019_SKU_TN.jpg
3578114
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

Documents

Documents

Number Description Options
10000127898
RAPID'E. coli O157:H7, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7274
Complete Solutions for Service Laboratories