ddPCR Gene Expression Assays

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Overview

ddPCR Gene Expression Assays enable accurate quantification of small fold changes in gene expression, for e.g. expression differences that are 2-fold or lower, for detecting targets that may be present at low levels of abundance or samples that contain substances that can inhibit a PCR reaction. ddPCR Gene Expression Assays are available as probe or EvaGreen Assays. Protocols are provided for use in One-Step or Two-Step RT-ddPCR protocols.

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Description

Description

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ddPCR Gene Expression Assays enable accurate quantification of small fold changes in gene expression, for e.g. expression differences that are 2-fold or lower, for detecting targets that may be present at low levels of abundance or samples that contain substances that can inhibit a PCR reaction. ddPCR Gene Expression Assays are available as probe or EvaGreen Assays. Protocols are provided for use in One-Step or Two-Step RT-ddPCR protocols. Find and buy ddPCR Gene Expression Assays.

Key Benefits

  • Enables detection of small fold changes in expression
  • Choose from more than 60,000 gene expression assays for human, rat, and mouse
  • Probe or EvaGreen® chemistry

Sample Data
6512-GENE-A
6512-GENE-B
6512-GENE-C

Robust PrimePCR gene expression assays used in ddPCR with EvaGreen provide precise quantification of 18 reference genes. A, 1-D fluorescence amplitude plot shows results of a two-step reverse transcription ddPCR reference assay panel run on human brain RNA. The black clusters on the plot represent the negative droplets and the blue clusters represent the droplets that are positive for the respective reference assay. B, concentration plot shows various gene concentrations of cDNA calculated as copies/µl on a log10 scale. C, calculated RNA concentrations in the original human brain RNA sample for each reference gene. All error bars are generated by QuantaSoft software and represent the 95% confidence interval.

Packaging Options

ddPCR Gene Expression Probe Assays are available with a FAM or HEX fluorophore. Reference assays with the complimentary fluorophore are available. These assays have been designed for maximum specificity and transcript coverage. Assays are available for human, mouse, and rat. Each tube contains 18uM primers and 5uM probe.

Catalog# Product Name Reaction Size
10031252 ddPCR GEX FAM Assay 200
10031253 ddPCR GEX FAM Assay 1000
10031254 ddPCR GEX FAM Assay 2500
10031255 ddPCR GEX HEX Assay 200
10031256 ddPCR GEX HEX Assay 1000
10031257 ddPCR GEX HEX Assay 2500

ddPCR Gene Expression Assays (EvaGreen) consists of unlabeled PCR primer pairs for use with dye-based chemistry such as the ddPCR EvaGreen Supermix. Individual assays are provided as a 20x stock solution. Each tube contains 5uM each primer.

Catalog# Product Name Reaction Size
10031258 ddPCR GEX EvaGreen Assay 200
10031259 ddPCR GEX EvaGreen Assay 1000
10031260 ddPCR GEX EvaGreen Assay 2500

 

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Resources

Documents

Documents

Number Description Options
10048730
PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A
10041702
PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A