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You have determined that electrophoresis is the next step in your experiment. With so many variables to consider before picking the gels, how do you know what the best gel is for your application? Here is an easy guide that takes you step by step through your selection process.
WHAT IS THE PURPOSE OF YOUR EXPERIMENT?
-
Nucleic Acid Analysis
-
Protein Analysis (SDS-Page)
-
Isoelectric Separation
WHAT IS THE SIZE OF YOUR TARGET PROTEIN?
-
Target Protein
Size <15 kD
-
Target Protein
Size 10–250 kD
-
Target Protein
Size >250 kD
WHAT'S THE BEST GEL CHEMISTRY FOR YOUR SAMPLE AND APPLICATION?
-
Sample buffer with high salt and high
nonionic detergent concentration*
- Bis-Tris Gels
(MOPS, MES Buffer)*Buffer exchange or dialysis can reduce salt and ionic detergents and lets the sample be run on TGX Gels
WHAT'S THE BEST PERCENTAGE
GEL FOR YOUR APPLICATION?-
Resolution of proteins <50 kD
(MES Buffer) -
Resolution of proteins >50 kD
(MOPS Buffer)
HOW MANY SAMPLES DO YOU WANT TO RUN?
-
5–12 Samples
-
More than 12 samples
Use Midi-Format Gels
Criterion Gels
-
Use Midi-Format Gels
Criterion Gels
Available Well Volumes:
12+2 wells: 45 µl 18 wells: 30 µl 26 wells: 15 µl - Bis-Tris Gels
-
Sample buffer with low salt
and low nonionic detergents
- Mini-PROTEAN TGX Gels
- Mini-PROTEAN TGX Stain-Free Gels
(Laemmli/Tris/Glycine SDS) -
- Faster run times
- Better transfer efficiency
WHAT'S THE BEST PERCENTAGE
GEL FOR YOUR APPLICATION?-
Size range between
proteins is narrowSingle Percentage Gels -
Size range between
proteins is broadGradient Gels
HOW MANY SAMPLES DO YOU WANT TO RUN?
-
5–12 Samples
-
More than 12 samples
-
Use Mini-Format Gels
Mini-PROTEAN TGX Precast Gels 
Use Midi-Format Gels
Criterion TGX Precast Gels
Available Well Volumes:
10 wells: 50 µl/30 µl 12 wells: 20 µl 15 wells: 15 µl Available Well Volumes:
12+2 wells: 45 µl 18 wells: 30 µl 26 wells: 15 µl
