Mini-PROTEAN TGX Stain-Free Precast Gels

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lsr_MiniProStainfree_new.jpg

Overview

Mini-PROTEAN TGX Stain-Free precast gels for polyacrylamide gel electrophoresis (PAGE) allow for fast run times, high transfer efficiency, and rapid detection of proteins with stain-free enabled imagers.
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Description

Description

Stain-Free

What Is Stain-Free Technology?

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Mini-PROTEAN TGX Stain-Free Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.

Features and Benefits of Mini-PROTEAN TGX Stain-Free Precast Gels

  • Run up to 4 Mini-PROTEAN TGX Stain-Free Protein Gels using the Mini-PROTEAN Tetra Cell
  • Run times as short as 15 minutes
  • Transfer proteins in as little as 15 minutes; verify transfer to membrane with a stain-free enabled imager
  • Inexpensive Laemmli buffer system, low running costs
  • Gel images and complete analysis in less than 5 minutes after electrophoresis
  • Comparable sensitivity to Coomassie stain
  • Better reproducibility and quantitation compared to staining procedures

Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels

  • 1-D polyacrylamide gel electrophoresis for protein separation
  • Accurate molecular weight estimation
  • Robust system for complex samples
  • One gel for western blotting, staining, and mass spectrometry

More Information

Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.

Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.

The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.

Ready to switch from Life Technologies NuPage or Bio-Rad’s Ready Gel Precast Gels? Use the the Interactive Gel Selector to find equivalent Mini-PROTEAN TGX Stain-Free Precast Gels.

Gel opening lever (4560000), sold separately, is 100% aluminum and recyclable

Ordering

Ordering

items

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456-8021_tn.jpg
4568021
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
456-8023_tn.jpg
4568023
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8024_tn.jpg
4568024
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8025_tn.jpg
4568025
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8026_tn.jpg
4568026
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
456-8031_tn.jpg
4568031
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
456-8033_tn.jpg
4568033
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8034_tn.jpg
4568034
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8035_tn.jpg
4568035
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8036_tn.jpg
4568036
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8041_tn.jpg
4568041
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8043_tn.jpg
4568043
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
456-8044_tn.jpg
4568044
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8045_tn.jpg
4568045
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8046_tn.jpg
4568046
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8081_tn.jpg
4568081
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8083_tn.jpg
4568083
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
17000546
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
456-8084_tn.jpg
4568084
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
17000927
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
456-8085_tn.jpg
4568085
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8086_tn.jpg
4568086
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8091_tn.jpg
4568091
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8093_tn.jpg
4568093
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
17000928
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
456-8094_tn.jpg
4568094
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
17000436
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
456-8095_tn.jpg
4568095
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8096_tn.jpg
4568096
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
17000435
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
456-8101_tn.jpg
4568101
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568103
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8104_tn.jpg
4568104
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8104_tn.jpg
4568105
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8106_tn.jpg
4568106
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8121_tn.jpg
4568121
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8123_tn.jpg
4568123
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8124_tn.jpg
4568124
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8125_tn.jpg
4568125
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
456-8126_tn.jpg
4568126
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
456-8021S_tn.jpg
4568021S
Discontinued item
Replaced with 7.5% Mini-PROTEAN TGX Stain-Free Protein Gels, 7 cm IPG/prep well, 450 µl, pkg 10 (4568021)
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4568023S
Discontinued item
Replaced with 7.5% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568023)
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4568024S
Discontinued item
Replaced with 7.5% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 50 µl, pkg 10 (4568024)
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4568025S
Discontinued item
Replaced with 7.5% Mini-PROTEAN TGX Stain-Free Protein Gels, 12-well, 20 µl, pkg 10 (4568025)
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4568026S
Discontinued item
Replaced with 7.5% Mini-PROTEAN TGX Stain-Free Protein Gels, 15-well, 15 µl, pkg 10 (4568026)
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4568029
Discontinued item
Replaced with 7.5% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568023)
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4568029S
Discontinued item
Replaced with 7.5% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568023)
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4568031S
Discontinued item
Replaced with 10% Mini-PROTEAN TGX Stain-Free Protein Gels, 7 cm IPG/prep well, 450 µl, pkg 10 (4568031)
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4568033S
Discontinued item
Replaced with 10% Mini-PROTEAN TGX Stain-Free Gel 10-well 30 µl, pkg 10 (4568033)
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4568034S
Discontinued item
Replaced with 10% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 50 µl, pkg 10 (4568034)
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4568035S
Discontinued item
Replaced with 10% Mini-PROTEAN TGX Stain-Free Protein Gels, 12-well, 20 µl, pkg 10 (4568035)
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4568036S
Discontinued item
Replaced with 10% Mini-PROTEAN TGX Stain-Free Protein Gels, 15-well, 15 µl, pkg 10 (4568036)
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4568039
Discontinued item
Replaced with 10% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568033)
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4568039S
Discontinued item
Replaced with 10% Mini-PROTEAN TGX Stain-Free Gel 10-well 30 µl, pkg 10 (4568033)
456-8041S_tn.jpg
4568041S
Discontinued item
Replaced with 12% Mini-PROTEAN TGX Stain-Free Protein Gels, 7 cm IPG/prep well, 450 µl, pkg 10 (4568041)
456-8043S_tn.jpg
4568043S
Discontinued item
Replaced with 12% Mini-PROTEAN TGX Stain-Free Gel 10-well 30 µl, pkg 10 (4568043)
456-8044S_tn.jpg
4568044S
Discontinued item
Replaced with 12% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 50 µl, pkg 10 (4568044)
456-8045S_tn.jpg
4568045S
Discontinued item
Replaced with 12% Mini-PROTEAN TGX Stain-Free Protein Gels, 12-well, 20 µl, pkg 10 (4568045)
456-8046S_tn.jpg
4568046S
Discontinued item
Replaced with 12% Mini-PROTEAN TGX Stain-Free Protein Gels, 15-well, 15 µl, pkg 10 (4568046)
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4568049
Discontinued item
Replaced with 12% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568043)
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4568049S
Discontinued item
Replaced with 12% Mini-PROTEAN TGX Stain-Free Gel 10-well 30 µl, pkg 10 (4568043)
456-8081s_tn.jpg
4568081S
Discontinued item
Replaced with 4–15% Mini-PROTEAN TGX Stain-Free Protein Gels, 7 cm IPG/prep well, 450 µl, pkg 10 (4568081)
456-8083s_tn.jpg
4568083S
Discontinued item
Replaced with 4–15% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568083)
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4568084S
Discontinued item
Replaced with 4–15% Mini-PROTEAN TGX Stain-Free 10-well 50 µl, pkg 10 (4568084)
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4568085S
Discontinued item
Replaced with 4–15% Mini-PROTEAN TGX Stain-Free 12-well 20 µl, pkg 10 (4568085)
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4568086S
Discontinued item
Replaced with 4–15% Mini-PROTEAN TGX Stain-Free 15-well 15 µl, pkg 10 (4568086)
456-8089_tn.jpg
4568089
Discontinued item
Replaced with 4–15% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568083)
456-8089s_tn.jpg
4568089S
Discontinued item
Replaced with 4–15% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568083)
456-8091s_tn.jpg
4568091S
Discontinued item
Replaced with 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels, 7 cm IPG/prep well, 450 µl, pkg 10 (4568091)
456-8093s_tn.jpg
4568093S
Discontinued item
Replaced with 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568093)
456-8094s_tn.jpg
4568094S
Discontinued item
Replaced with 4–20% Mini-PROTEAN TGX Stain-Free Gel 10-well 50 µl, pkg 10 (4568094)
456-8095s_tn.jpg
4568095S
Discontinued item
Replaced with 4–20% Mini-PROTEAN TGX Stain-Free Gel 12-well 20 µl, pkg 10 (4568095)
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4568096S
Discontinued item
Replaced with 4–20% Mini-PROTEAN TGX Stain-Free Gel 15-well 15 µl, pkg 10 (4568096)
456-8099_tn.jpg
4568099
Discontinued item
Replaced with 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568093)
456-8099s_tn.jpg
4568099S
Discontinued item
Replaced with 4–20% Mini-PROTEAN TGX Stain-Free Gel 10-well 30 µl pkg 10 (4568093)
456-8101s_tn.jpg
4568101S
Discontinued item
Replaced with 8–16% Mini-PROTEAN TGX Stain-Free Protein Gels, 7 cm IPG/prep well, 450 µl, pkg 10 (4568101)
456-8103s_tn.jpg
4568103S
Discontinued item
Replaced with 8–16% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568103)
456-8104s_tn.jpg
4568104S
Discontinued item
Replaced with 8–16% Mini-PROTEAN TGX Stain-Free Gel 10-well 50 µl, pkg 10 (4568104)
456-8105s_tn.jpg
4568105S
Discontinued item
Replaced with 8–16% Mini-PROTEAN TGX Stain-Free Gel 12-well 20 µl, pkg 10 (4568105)
456-8106s_tn.jpg
4568106S
Discontinued item
Replaced with 8–16% Mini-PROTEAN TGX Stain-Free Gel 15-well, 15 µl, pkg 10 (4568106)
456-8109_tn.jpg
4568109
Discontinued item
Replaced with 8–16% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568103)
456-8109s_tn.jpg
4568109S
Discontinued item
Replaced with 8–16% Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568103)
456-8121S_tn.jpg
4568121S
Discontinued item
Replaced with Any kD Mini-PROTEAN TGX Stain-Free Protein Gels, 7 cm IPG/prep well, 450 µl, pkg 10 (4568121)
456-8123S_tn.jpg
4568123S
Discontinued item
Replaced with Any kD Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568123)
456-8124S_tn.jpg
4568124S
Discontinued item
Replaced with Any kD Mini-PROTEAN TGX Stain-Free 10-well 50 µl, pkg 10 (4568124)
456-8125S_tn.jpg
4568125S
Discontinued item
Replaced with Any kD Mini-PROTEAN TGX Stain-Free 12-well 20 µl, pkg 10 (4568125)
456-8126S_tn.jpg
4568126S
Discontinued item
Replaced with Any kD Mini-PROTEAN TGX Stain-Free 15-well 15 µl, pkg 10 (4568126)
456-8129_tn.jpg
4568129
Discontinued item
Replaced with Any kD Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568123)
456-8129S_tn.jpg
4568129S
Discontinued item
Replaced with Any kD Mini-PROTEAN TGX Stain-Free Protein Gels, 10-well, 30 µl, pkg 10 (4568123)

Accessories

Accessories

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4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

Kits & Reagents

Kits & Reagents

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1610737
30 ml, premixed protein sample buffer for SDS-PAGE
161-0738_tn.jpg
1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
161-0732_tn.jpg
(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
161-0376_tn.jpg
1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

Documents

Documents

Number Description Options
6427
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
6356
Traditional vs. Stain-Free Western Blotting
10026447
Mini-PROTEAN® TGX™ Precast Gel Quick Start Guide, Rev C
M1658100
Mini-PROTEAN Precast Gels Instruction Manual and Application Guide, Rev F
5974
Mini-PROTEAN® and Criterion™ TGX Stain-Free™ Precast Gels Product Information Sheet, Rev E
5932
Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E
5934
NuPAGE Bis-Tris Precast Gels (MOPS Buffer) to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev G
6040
Electrophoresis Guide, Interactive PDF, Rev C
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6390
General Stain-Free Western Blotting Protocol
[ Add to Cart (Free) ]
6434
Western Blot Normalization Using Image Lab™ Software, Quick Start Guide
[ Add to Cart (Free) ]
6166
Multiplex Fluorescent Blot Detection: A Troubleshooting Guide
5910
Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B
5810
Compatibility of the Criterion Stain-Free Gel Imaging System with Mass Spectrometric Protein Analysis
6453
Utilize Stain-Free Technology in a Variety of Workflows, Rev B
[ Add to Cart (Free) ]
6494
Protein Purification Workflow with the NGC Chromatography System and Stain-Free Gels Brochure
[ Add to Cart (Free) ]
6507
TGX and TGX Stain-Free Gels: Publications List