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      Mini-PROTEAN TGX Stain-Free Precast Gels

      lsr_MiniProStainfree_new.jpg

      Overview

      Mini-PROTEAN TGX Stain-Free precast gels for polyacrylamide gel electrophoresis (PAGE) allow for fast run times, high transfer efficiency, and rapid detection of proteins with stain-free enabled imagers.
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      Description

      Description

      Stain-Free

      What Is Stain-Free Technology?

      Find out more »

      Mini-PROTEAN TGX Stain-Free Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.

      Features and Benefits of Mini-PROTEAN TGX Stain-Free Precast Gels

      • Run up to 4 Mini-PROTEAN TGX Stain-Free Protein Gels using the Mini-PROTEAN Tetra Cell
      • Run times as short as 15 minutes
      • Transfer proteins in as little as 15 minutes; verify transfer to membrane with a stain-free enabled imager
      • Inexpensive Laemmli buffer system, low running costs
      • Gel images and complete analysis in less than 5 minutes after electrophoresis
      • Comparable sensitivity to Coomassie stain
      • Better reproducibility and quantitation compared to staining procedures

      Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels

      • 1-D polyacrylamide gel electrophoresis for protein separation
      • Accurate molecular weight estimation
      • Robust system for complex samples
      • One gel for western blotting, staining, and mass spectrometry

      More Information

      Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.

      Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.

      The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.

      Ready to switch from Life Technologies NuPage or Bio-Rad’s Ready Gel Precast Gels? Use the the Interactive Gel Selector to find equivalent Mini-PROTEAN TGX Stain-Free Precast Gels.

      Gel opening lever (4560000), sold separately, is 100% aluminum and recyclable

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      456-8021_tn.jpg
      (Discontinued) 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 7 cm IPG/prep well, 450 µl
      4568021
      Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
      456-8023_tn.jpg
      (Discontinued) 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
      4568023
      Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8024_tn.jpg
      (Discontinued) 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 50 µl
      4568024
      Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8025_tn.jpg
      (Discontinued) 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 12 well, 20 µl
      4568025
      Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8026_tn.jpg
      (Discontinued) 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
      4568026
      Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
      456-8031_tn.jpg
      (Discontinued) 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 7 cm IPG/prep well, 450 µl
      4568031
      Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
      456-8033_tn.jpg
      (Discontinued) 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
      4568033
      Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8034_tn.jpg
      (Discontinued) 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 50 µl
      4568034
      Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8035_tn.jpg
      (Discontinued) 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 12 well, 20 µl
      4568035
      Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8036_tn.jpg
      (Discontinued) 10% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
      4568036
      Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8041_tn.jpg
      (Discontinued) 12% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 7 cm IPG/prep well, 450 µl
      4568041
      Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8043_tn.jpg
      (Discontinued) 12% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
      4568043
      Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
      456-8044_tn.jpg
      (Discontinued) 12% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 50 µl
      4568044
      Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8045_tn.jpg
      (Discontinued) 12% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 12 well, 20 µl
      4568045
      Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8046_tn.jpg
      (Discontinued) 12% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
      4568046
      Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8081_tn.jpg
      (Discontinued) 4–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 7 cm IPG/prep well, 450 µl
      4568081
      Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8083_tn.jpg
      (Discontinued) 4–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
      4568083
      Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
      (Discontinued) 4–15% Mini-PROTEAN® TGX Stain-Free™ Gels, 10-well, 30 µl, with Precision Plus Protein™ Unstained Standards
      17000546
      Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
      456-8084_tn.jpg
      (Discontinued) 4–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 50 µl
      4568084
      Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
      (Discontinued) 4–15% Mini-PROTEAN TGX Stain-Free™ Gels, 10-well, 50 µl, with Precision Plus Protein™ Unstained Standards
      17000927
      Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
      456-8085_tn.jpg
      (Discontinued) 4–15% Mini-PROTEAN&reg TGX Stain-Free™ Protein Gels, 12 well, 20 µl
      4568085
      Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8086_tn.jpg
      (Discontinued) 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels, 15 well, 15 µl
      4568086
      Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8091_tn.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 7 cm IPG/prep well, 450 µl
      4568091
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8093_tn.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
      4568093
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels, 10-well, 30 µl, with Precision Plus Protein™ Unstained Standards
      17000928
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
      456-8094_tn.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 50 µl
      4568094
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels, 10-well, 50 µl, with Precision Plus Protein™ Unstained Standards
      17000436
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
      456-8095_tn.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 12 well, 20 µl
      4568095
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8096_tn.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
      4568096
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      4-15-percent-mini-protean-tgx-stain-free-gels-17000435_thumb.jpg
      (Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels, 15-well, 15 µl, with Precision Plus Protein™ Unstained Standards
      17000435
      Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
      456-8101_tn.jpg
      (Discontinued) 8–16% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 7 cm IPG/prep well, 450 µl
      4568101
      Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8103_tn.jpg
      (Discontinued) 8–16% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
      4568103
      Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8104_tn.jpg
      (Discontinued) 8–16% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 50 µl
      4568104
      Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8104_tn.jpg
      (Discontinued) 8–16% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 12 well, 20 µl
      4568105
      Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8106_tn.jpg
      (Discontinued) 8–16% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
      4568106
      Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8121_tn.jpg
      (Discontinued) Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 7 cm IPG/prep well, 450 µl
      4568121
      Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8123_tn.jpg
      (Discontinued) Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 30 µl
      4568123
      Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8124_tn.jpg
      (Discontinued) Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 10 well, 50 µl
      4568124
      Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
      456-8125_tn.jpg
      (Discontinued) Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 12 well, 20 µl
      4568125
      Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
      456-8126_tn.jpg
      (Discontinued) Any kD™ Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 15 well, 15 µl
      4568126
      Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells

      Accessories

      Accessories

      Kits & Reagents

      Kits & Reagents

      161-0738_tn.jpg
      (Discontinued) Native Sample Buffer for Protein Gels, 30 ml
      1610738
      30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
      161-0732_tn.jpg
      (Discontinued) 10x Tris/Glycine/SDS
      1610732
      Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
      161-0376_tn.jpg
      (Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl
      1610376
      250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
      161-0363_tn.jpg
      (Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml
      1610363
      1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications

      Documents

      Documents

      Number Description Options
      6427
      Bio-Rad Stain-Free Western Blotting
      [ Add to Cart (Free) ]
      6356
      Traditional vs. Stain-Free Western Blotting
      10026447
      Mini-PROTEAN® TGX™ Precast Gel Quick Start Guide, Rev C
      5974
      Mini-PROTEAN® and Criterion™ TGX Stain-Free™ Precast Gels Product Information Sheet, Rev E
      5932
      Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E
      5934
      NuPAGE Bis-Tris Precast Gels (MOPS Buffer) to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev G
      6040
      Electrophoresis Guide, Interactive PDF, Rev C
      10040758
      Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
      6390
      General Stain-Free Western Blotting Protocol
      [ Add to Cart (Free) ]
      6434
      Western Blot Normalization Using Image Lab™ Software, Quick Start Guide
      [ Add to Cart (Free) ]
      6166
      Multiplex Fluorescent Blot Detection: A Troubleshooting Guide
      5910
      Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B
      5810
      Compatibility of the Criterion Stain-Free Gel Imaging System with Mass Spectrometric Protein Analysis
      6453
      Utilize Stain-Free Technology in a Variety of Workflows, Rev B
      [ Add to Cart (Free) ]
      6494
      Protein Purification Workflow with the NGC Chromatography System and Stain-Free Gels Brochure
      [ Add to Cart (Free) ]
      6507
      TGX and TGX Stain-Free Gels: Publications List