Description
Description
What is Stain-Free Technology?
Download the Complete Guide to Stain-Free Western Blotting and learn how it will revolutionize your research.
Mini-PROTEAN TGX Stain-Free Precast Gels are based on the long-shelf-life TGX (Tris-Glycine eXtended) formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with Bio-Rad stain-free imaging systems.
Features and Benefits of Mini-PROTEAN TGX Stain-Free Precast Gels
- Run up to 4 Mini-PROTEAN TGX Stain-Free Protein Gels using the Mini-PROTEAN Tetra Cell
- Run times as short as 15 minutes
- Transfer proteins in as little as 15 minutes; verify transfer to membrane with a stain-free enabled imager
- Inexpensive Laemmli buffer system, low running costs
- Gel images and complete analysis in less than 5 minutes after electrophoresis
- Comparable sensitivity to Coomassie stain
- Better reproducibility and quantitation compared to staining procedures
Applications and Uses of Mini-PROTEAN TGX Stain-Free Precast Gels
- 1-D polyacrylamide gel electrophoresis for protein separation
- Accurate molecular weight estimation
- Robust system for complex samples
- One gel for western blotting, staining, and mass spectrometry
More Information
Mini-PROTEAN TGX Stain-Free Precast Gels do not contain SDS. For standard denaturing electrophoresis use sample and running buffers containing SDS. To retain native protein conformation and activity these gels can be run with sample and running buffer that do not contain SDS.
Mini-PROTEAN TGX Stain-Free Gels retain Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Protein migration patterns under non-denaturing conditions do not provide accurate molecular weight estimations.
The trihalo compounds in Mini-PROTEAN TGX Stain-Free Gels react with tryptophan residues in proteins in a UV-induced 1-minute reaction to produce fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting and may be subsequently visualized in gels or on PVDF membranes multiple times for validation in a western blotting workflow. The use of stain-free imaging also allows for the normalization of bands to the total protein on a blot, eliminating the use of housekeeping proteins or stripping and reprobing.
Ready to make the switch to Stain-Free enabled precast gels? Use the Western Blot Selector Tool to easily find gels compatible for your next experiment.
Gel opening lever (4560000), sold separately, is 100% aluminum and recyclable
Ordering
Ordering
items
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4568021
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
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4568023
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568024
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568025
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568026
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
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4568031
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells
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4568033
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568034
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568035
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568036
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568041
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568043
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
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4568044
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568045
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568046
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568081
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568083
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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(Discontinued) 4–15% Mini-PROTEAN® TGX Stain-Free™ Gels, 10-well, 30 µl, with Precision Plus Protein™ Unstained Standards
17000546
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
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4568084
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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(Discontinued) 4–15% Mini-PROTEAN® TGX Stain-Free™ Gels, 10-well, 50 µl, with Precision Plus Protein™ Unstained Standards
17000927
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
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4568085
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568086
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568091
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568093
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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(Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels, 10-well, 30 µl, with Precision Plus Protein™ Unstained Standards
17000928
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
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4568094
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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(Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels, 10-well, 50 µl, with Precision Plus Protein™ Unstained Standards
17000436
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
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4568095
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568096
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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(Discontinued) 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels, 15-well, 15 µl, with Precision Plus Protein™ Unstained Standards
17000435
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 x 6.7 cm (W x L), for use with Mini-PROTEAN Electrophoresis Cells; includes 100 µl Precision Plus Protein Unstained Standards
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4568101
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568103
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568104
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568105
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568106
Pkg of 10, 8–16% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568121
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568123
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568124
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
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4568125
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells and stain-free enabled imagers
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4568126
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
Accessories
Accessories
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(Discontinued) Mini-PROTEAN® Cassette Opening Lever
4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes
Kits & Reagents
Kits & Reagents
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(Discontinued) 2x Laemmli Sample Buffer
1610737
30 ml, premixed protein sample buffer for SDS-PAGE
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(Discontinued) Native Sample Buffer for Protein Gels, 30 ml
1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue
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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl
1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
Documents
Documents
Login Required Number Description Options
6356
Traditional vs. Stain-Free Western Blotting
10026447
Mini-PROTEAN® TGX™ Precast Gel Quick Start Guide, Rev C
5974
Mini-PROTEAN® and Criterion™ TGX Stain-Free™ Precast Gels Product Information Sheet, Rev E
5932
Ready Gel to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev E
5934
NuPAGE Bis-Tris Precast Gels (MOPS Buffer) to Mini-PROTEAN TGX Precast Gels Catalog Number Conversion Chart, Rev G
6040
Electrophoresis Guide, Interactive PDF, Rev C
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6166
Multiplex Fluorescent Blot Detection: A Troubleshooting Guide
5910
Mini-PROTEAN TGX Precast Gel: A Gel for SDS-PAGE with Improved Stability — Comparison with Standard Laemmli Gels, Rev B
5810
Compatibility of the Criterion Stain-Free Gel Imaging System with Mass Spectrometric Protein Analysis
6494
Protein Purification Workflow with the NGC Chromatography System and Stain-Free Gels Brochure
6507
TGX and TGX Stain-Free Gels: Publications List