Ready Gel® IEF Precast Gels

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Ready Gel IEF Precast Gels

Overview

Migration Pattern for Ready Gel IEF Gels

The banding patterns indicate the optimal separation by isoelectric point (pI) for each acrylamide percentage in combination with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

Mini-Format Precast Gel Selection Guide
Refer to the following table to select the appropriate gel type and buffers for your polyacrylamide gel-based applications.
 
Gel Type


Applications



Sample Buffer Running Buffer
TGX™
TGX Stain-Free™



SDS-PAGE
Native PAGE




Laemmli, Native Tris/Glycine/SDS
Tris/Glycine

Tris-Tricine


SDS-PAGE



Tricine Tris/Tricine/SDS
IEF


IEF



IEF IEF Anode and Cathode buffers
Zymogram


Protease detection



Zymogram Tris/Glycine/SDS
TBE
TBE-Urea
      Nucleic acids         Nucleic Acid, TBE-Urea TBE  


Discontinued Product
Ready Gel IEF Gels have been replaced with
Criterion™ IEF Precast Gels.
Read full description

Ordering Information


Description

Discontinued Product

Ready Gel IEF Gels have been replaced with Criterion™ IEF Precast Gels

To find a recommended replacement gel for a particular discontinued Ready Gel, either:

  • Find the discontinued item on the Ready Gel Ordering tab with link to the corresonding Criterion Gel, or
  • Enter the catalog number of the discontinued item in the search bar of the Discontinued Products page

If no replacement gel is listed, please contact Technical Support for questions about transitioning to a new product.

 

Specifications

Gel dimensions (W x L x thickness) 8.6 x 6.8 x 0.1 cm
Cassette dimensions (W x L x thickness) 10 x 8 x 0.4 cm
Gel storage conditions Store flat at 4°C; do not freeze
Gel shelf life* ~26 weeks
* From date of manufacture.  

Ordering

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(Discontinued) pH 3–10 Ready Gel® IEF Gel, 10 well, 30 µl

1611111
Discontinued item
Replaced with pH 3–10 Criterion™ IEF Gel, 12+2 well, 45 µl

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) pH 3–10 Ready Gel® IEF Gel, 15 well, 15 µl

1611129
Discontinued item
Replaced with pH 3–10 Criterion™ IEF Gel, 18 well, 30 µl

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) pH 5–8 Ready Gel® IEF Gel, 10 well, 30 µl

1611112
Discontinued item
Replaced with pH 5–8 Criterion IEF Gel 18-well 30 µl (3450076)

This product is no longer available. Please contact technical support for more information on replacement product.

Accessories

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list price. If the problem persists, please call customer service at or send an
email to

Mini-PROTEAN® Cassette Opening Lever

4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

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$5.00 
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Kits & Reagents

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10x IEF Anode Buffer, 250 ml

1610761
Pkg of 1, 250 ml, electrophoresis buffer, 7 mM phosphoric acid, for use in isoelectric focusing

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$11.00 
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10x IEF Cathode Buffer, 250 ml

1610762
Pkg of 1, 250 ml, electrophoresis buffer, 20 mM lysine, 20 mM arginine, for use in isoelectric focusing

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$66.00 
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IEF Sample Buffer for Vertical Gels, 30 ml

1610763
30 ml, protein sample buffer, 50% glycerol, for use in IEF with both liquid and lyophilized samples

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$14.00 
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Documents

Number Description Options
2364 Ready Gel System Resource Guide Addendum, Rev A Click to download
2144 Ready Gel® System Resource Guide Click to download
LIT188 Instruction Manual, Ready Gel Precast Gels Application Guide, Rev H Click to download
4006105 Instruction Manual, Isoelectric Focusing Gel Sample Buffer (IEF Gel), Rev C Click to download

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