Criterion™ IEF Precast Gels

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Criterion IEF Precast Gels

Overview

Specifications
Resolving Gel
(%T)
Stacking Gel
(%T)
Crosslinker
(%C)
pH Range Optimal Separation Range
5% None 3.3% pH 3–10 pH ~3.5–8.5
5% None 3.3% pH 5–8 pH ~4.6–7.8
Criterion IEF Precast Gels

Criterion gel migration chart.

Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel.
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Ordering Information


Description

Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel. Proteins migrate to their pI, the pH at which their net charge is zero. IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions.

Criterion precast gels include a broad selection of 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results with fast setup, loading, and run times. Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion Dodeca cell. The Criterion blotter offers a variety of convenient features, such as two electrode configurations and a convenient gel/blot assembly tray, for blotting applications.


Specifications

Gel dimensions 13.3 x 8.7 cm (W x L); 1.0 mm thick
Cassette dimensions 15.0 x 10.6 cm (W x L); 5.3 mm thick
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Gel storage conditions 4°C Store flat; do not freeze
Gel shelf life* 26 weeks
Buffer volume Upper, 60 ml; lower, 400 ml

Ordering

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pH 3–10 Criterion™ IEF Gel, 12+2 well

3450071
Pkg of 1, pH 3–10 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$43.00 
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pH 3–10 Criterion™ IEF Gel, 18 well, 30 µl

3450072
Pkg of 1, pH 3–10 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$43.00 
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(Discontinued) pH 3–10 Criterion™ IEF Gel, 26 well, 15 µl

3450073
Pkg of 1, pH 3–10 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

This product is no longer available. Please contact technical support for more information on replacement product.

The following replacement is recommended:

  • pH 3–10 Criterion™ IEF Gel, 18 well, 30 µl (3450072)
pH 5–8 Criterion™ IEF Gel, 18 well, 30 µl

3450076
Pkg of 1, pH 5–8 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

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$66.00 
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Accessories

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Mini-PROTEAN® Cassette Opening Lever

4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

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$5.00 
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Kits & Reagents

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10x IEF Anode Buffer, 250 ml

1610761
Pkg of 1, 250 ml, electrophoresis buffer, 7 mM phosphoric acid, for use in isoelectric focusing

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$11.00 
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10x IEF Cathode Buffer, 250 ml

1610762
Pkg of 1, 250 ml, electrophoresis buffer, 20 mM lysine, 20 mM arginine, for use in isoelectric focusing

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$66.00 
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IEF Sample Buffer for Vertical Gels, 30 ml

1610763
30 ml, protein sample buffer, 50% glycerol, for use in IEF with both liquid and lyophilized samples

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$14.00 
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Documents

Number Description Options
2788 Criterion Blotting Tray Insert, Rev A Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download

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