Criterion™ IEF Precast Gels

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Overview

Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel.
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Description

Description

Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel. Proteins migrate to their pI, the pH at which their net charge is zero. IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions.

Criterion precast gels include a broad selection of 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results with fast setup, loading, and run times. Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion Dodeca cell. The Criterion blotter offers a variety of convenient features, such as two electrode configurations and a convenient gel/blot assembly tray, for blotting applications.

Specifications

Specifications

Gel dimensions
13.3 x 8.7 cm (W x L); 1.0 mm thick
Cassette dimensions
15.0 x 10.6 cm (W x L); 5.3 mm thick
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
4°C Store flat; do not freeze
Gel shelf life*
26 weeks
Buffer volume
Upper, 60 ml; lower, 400 ml

Ordering

Ordering

items

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3450071
Pkg of 1, pH 3–10 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450072
Pkg of 1, pH 3–10 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450076
Pkg of 1, pH 5–8 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Accessories

Accessories

Kits & Reagents

Kits & Reagents

Documents

Documents

Number Description Options
2788
Criterion Blotting Tray Insert, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H