Criterion IEF Precast Gels
Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel.
Overview
Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel. Proteins migrate to their pI, the pH at which their net charge is zero. IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions.
Criterion precast gels include a broad selection of 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results with fast setup, loading, and run times. Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion Dodeca cell. The Criterion blotter offers a variety of convenient features, such as two electrode configurations and a convenient gel/blot assembly tray, for blotting applications.
Specifications
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Pkg of 1, pH 3–10 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Pkg of 1, pH 5–8 precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Accessories
Kits & Reagents
Pkg of 1, 250 ml, electrophoresis buffer, 20 mM lysine, 20 mM arginine, for use in isoelectric focusing
