Criterion™ TBE-Urea Precast Gels

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Overview

TBE-urea gels maintain denaturing conditions for analysis of single-stranded DNA and RNA. Nucleic acids between 60 and 200 bases are resolved as sharp, distinct bands.
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Description

Description

TBE-urea gels maintain denaturing conditions for analysis of single-stranded DNA and RNA. Nucleic acids between 60 and 200 bases are resolved as sharp, distinct bands. Applications include oligonucleotide purity analysis, RNase protection assays, and northern blotting.

Criterion precast gels include a broad selection of 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results with fast setup, loading, and run times. Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion Dodeca cell. The Criterion blotter offers a variety of convenient features, such as two electrode configurations and a convenient gel/blot assembly tray, for blotting applications.

Specifications

Specifications

Gel dimensions
13 3 x 8 7 cm (W x L); 1 0 mm thick
Cassette dimensions
15 0 x 10 6 cm (W x L); 5 3 mm thick
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
4°C Store flat; do not freeze
Gel shelf life*
12 weeks
Buffer volume
Upper, 60 ml; lower, 400 ml

Ordering

Ordering

items

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3450086
Pkg of 1, 5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450088
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450089
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450090
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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3450091
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Image
345-0092_tn.jpg
3450092
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
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345-0093_tn.jpg
3450093
Pkg of 1, 15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

Accessories

Accessories

Kits & Reagents

Kits & Reagents

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1610767
10 ml, 5x DNA loading buffer, premixed, for nondenaturing TBE gels, pH 8.0
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1610768
30 ml, 2x TBE-urea sample loading buffer, premixed, for denaturing ssDNA and RNA, pH 8.0

Documents

Documents

Number Description Options
2788
Criterion Blotting Tray Insert, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C