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Description
Description
Use Phosphate Buffered Saline (PBS) with 1% (w/v) Casein for blocking membranes for blotting. Blocking buffers containing casein may provide lower backgrounds than buffers containing low fat milk, and casein is recommended for applications using biotin-avidin complexes. When using alkaline phosphatase as a reporter in western blots, it is recommended that Phosphate Buffered Saline (PBS) be replaced with Tris Buffered Saline (TBS).
Related Products
- PrecisionAb™ Validated Western Blotting Antibodies
- Criterion™ Staining/Blotting Trays with Lids (3459921)
- 10x Tris Buffered Saline (TBS) (1706435)
- 10x Phosphate Buffered Saline (PBS) (1610780)
- 1x Tris Buffered Saline (TBS) with 1% Casein (1610782)
Related Categories
- Protein Gels
- Western Blotting Membranes and Filter Paper
- Semi-Dry and Rapid Blotting Systems
- Wet/Tank Blotting Systems
- Power Supplies
- Transfer Buffers
- Immunodetection Reagents and Kits
- Gel and Western Imaging Systems
Supporting Documents
- Mini-PROTEAN® Precast Gels Instruction Manual and Application Guide
- Criterion Gel Application Guide Instruction Manual
- Mini Trans-Blot® Cell Instruction Manual
- Trans-Blot SD Cell Quick Reference Guide
- Trans-Blot SD Semi-Dry Transfer Cell Instruction Manual
