Overview
The Vericheck ddPCR Empty-Full Capsid Kit enables a quick and precise measurement of the empty-full capsid ratio that is needed to produce AAV vectors. The empty-full capsid ratio is a Critical Quality Attribute (CQA) in AAV vector production since high amounts of empty capsids may cause unwanted immunotoxicity. This kit simultaneously provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low-sample input, fast turnaround time, and easy data analysis.
Features & Benefits
- Sample type compatibility — crude lysate and purified
- Low sample input — only 106 virus particles required to get results
- Fast turnaround time — up to 14 samples per plate in less than 1 day
- High precision — coefficient of variation (CV) less than 5%
- Simplified data analysis – streamlined process providing simultaneous results for genome titer, capsid titer, and empty-full ratio
Applications & Uses
- Simply and precisely measure empty-full ratio with one assay
- Optimized to provide precision throughout the AAV characterization process
Instrument Compatibility
| (Bundles or different component sets) | Catalog Number |
| QX ONE Droplet Digital PCR System | 12006536 |
| QX200 AutoDG Droplet Digital PCR System consisting of: QX200 Droplet Reader Automated Droplet Generator |
1864100 1864003 1864101 |
| QX600 Droplet Digital PCR Reader | 12013328 |
| QX Manager Software, Regulatory Edition | 12012172 |
| QX Manager Software, Standard Edition | 12012171 |
| QX ONE Software, Regulatory Edition | 12012078 |
| QX Manager, Premium Edition | 12018108 |
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With the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kit requires only small sample volume of crude lysate or purified samples to simultaneously measure capsid and viral titer for AAV serotype 5.
With the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kit requires only small sample volume of crude lysate or purified samples to simultaneously measure capsid and viral titer for AAV serotype 9.
With the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kits require only small volume of crude lysate or purified samples, which is ideal for any steps in the AAV vector manufacturing process.
With the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kits require only small volume of crude lysate or purified samples, which is ideal for any steps in the AAV vector manufacturing process.
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Determine results of empty and full capsids as well as genome and viral titer. with confidence