
Description
Description
The Vericheck ddPCR Empty-Full Capsid Kit enables a quick and precise measurement of the empty-full capsid ratio that is needed to produce AAV vectors. The empty-full capsid ratio is a Critical Quality Attribute (CQA) in AAV vector production since high amounts of empty capsids may cause unwanted immunotoxicity. This kit simultaneously provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low-sample input, fast turnaround time, and easy data analysis.
Features & Benefits
- Sample type compatibility — Crude lysate and purified
- Low sample input — Only 106 virus particles required to get results
- Fast turnaround — Up to 14 samples per plate in less than 1 day
- High precision — Coefficient of variation (CV) less than 5%
- Simplified data analysis – Streamlined process providing simultaneous results for genome titer, capsid titer, and empty-full ratio
Product Contents
The Vericheck ddPCR Empty-Full Capsid AAV5 Kit (17010072) contains the following products:
Catalog Number | Description | Quantity |
12022102 | Vericheck ddPCR Empty Full Capsid Reagent Box | 1 |
16012910 | 20x ddPCR ITR2 Assay | 1 |
16012911 | 20x ddPCR Capsid Detection Assay | 1 |
12022016 | Ligase | 1 |
12022074 | 10x Ligase Buffer | 1 |
12022017 | Dilution Buffer | 1 |
12022018 | Stop Solution | 1 |
16012872 | 10x PLA Splint | 1 |
10042051 | Dnase | 1 |
12022020 | 10x Dnase Buffer | 1 |
12007991 | ddPCR Supermix for Probes No dUTP 0.7ML | 2 |
Catalog Number | Description | Quantity |
12022098 | Vericheck ddPCR Empty Full Capsid Kit AAV5 Box | 1 |
12022088 | AAV5 Antibody 1 | 1 |
12022075 | AAV5 Antibody 2 | 1 |
12022369 | AAV5 Positive Control | 1 |
12022097 | KIT AAV9 Vericheck ddPCR EF Capsid | 1 |
12022099 | AAV9 Antibody 1 | 1 |
12022036 | AAV9 Antibody 2 | 1 |
12022403 | AAV9 Positive Control Labelled | 1 |
Instrument Compatibility
Instrument | Catalog Number |
QX ONE Droplet Digital PCR System | 12006536 |
QX200 AutoDG Droplet Digital PCR System consisting of: QX200 Droplet Reader Automated Droplet Generator |
1864100 1864003 1864101 |
QX600 Droplet Digital PCR Reader | 12013328 |
QX Manager Software, Regulatory Edition | 12012172 |
QX ONE Software, Regulatory Edition | 12012078 |
QX Manager, Premium Edition | 12018108 |