Vericheck ddPCR Empty-Full Capsid AAV5 Kit #17010072

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Vericheck Empty Full Capsid AAV5 Kit

Overview

With the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kit requires only small sample volume of crude lysate or purified samples to simultaneously measure capsid and viral titer for AAV serotype 5.

Description

Description

The Vericheck ddPCR Empty-Full Capsid Kit enables a quick and precise measurement of the empty-full capsid ratio that is needed to produce AAV vectors. The empty-full capsid ratio is a Critical Quality Attribute (CQA) in AAV vector production since high amounts of empty capsids may cause unwanted immunotoxicity. This kit simultaneously provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low-sample input, fast turnaround time, and easy data analysis.

Features & Benefits

  • Sample type compatibility — Crude lysate and purified
  • Low sample input — Only 106 virus particles required to get results
  • Fast turnaround — Up to 14 samples per plate in less than 1 day
  • High precision — Coefficient of variation (CV) less than 5%
  • Simplified data analysis – Streamlined process providing simultaneous results for genome titer, capsid titer, and empty-full ratio

Product Contents

The Vericheck ddPCR Empty-Full Capsid AAV5 Kit (17010072) contains the following products:

Catalog Number Description Quantity
12022102 Vericheck ddPCR Empty Full Capsid Reagent Box 1
16012910 20x ddPCR ITR2 Assay 1
16012911 20x ddPCR Capsid Detection Assay 1
12022016 Ligase 1
12022074 10x Ligase Buffer 1
12022017 Dilution Buffer 1
12022018 Stop Solution 1
16012872 10x PLA Splint 1
10042051 Dnase 1
12022020 10x Dnase Buffer 1
12007991 ddPCR Supermix for Probes No dUTP 0.7ML 2
Catalog Number Description Quantity
12022098 Vericheck ddPCR Empty Full Capsid Kit AAV5 Box 1
12022088 AAV5 Antibody 1 1
12022075 AAV5 Antibody 2 1
12022369 AAV5 Positive Control 1
12022097 KIT AAV9 Vericheck ddPCR EF Capsid 1
12022099 AAV9 Antibody 1 1
12022036 AAV9 Antibody 2 1
12022403 AAV9 Positive Control Labelled 1

Instrument Compatibility

Instrument Catalog Number
QX ONE Droplet Digital PCR System 12006536
QX200 AutoDG Droplet Digital PCR System consisting of:
QX200 Droplet Reader
Automated Droplet Generator
1864100
1864003
1864101
QX600 Droplet Digital PCR Reader 12013328
QX Manager Software, Regulatory Edition 12012172
QX ONE Software, Regulatory Edition 12012078
QX Manager, Premium Edition 12018108

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