Vericheck ddPCR Empty-Full Capsid AAV2 Kit #17010081

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Vericheck ddPCR Empty

Overview

With the absolute quantification of Droplet Digital™ PCR, the Vericheck ddPCR Empty-Full Capsid Kits require only small volume of crude lysate or purified samples, which is ideal for any steps in the AAV vector manufacturing process.

Description

Description

The Vericheck ddPCR Empty-Full Capsid Kit provides enables a quick and simple check of the empty-full capsid ratio that is needed for the production of produce AAV vectors. The Empty full capsid ratio is a CQA in AAV vector production, as since high amounts of empty capsids may cause unwanted immunotoxicity. This kit provides consistent and reliable capsid content measurements across the AAV production process with high precision, multi-sample type compatibility, low low-sample input, fast turnaround time, and easy data analysis

Features & Benefits

  • Sample Type Compatibility — crude lysate and purified sample.
  • Low Sample Input — only 106 virus particles required to get results
  • Fast Turnaround — up to 14 samples per plate in less than 1 day
  • High Precision — CVs closer to 2%
  • Data Analysis — simplified workflow with results for genome titer, capsid titer, and empty full ratio

Product Contents

Catalog Number Description Quantity
12022102 Vericheck ddPCR Empty Full Capsid Reagent Box 1
16012910 20x ddPCR ITR2 Assay 1
16012911 20x ddPCR Capsid Detection Assay 1
12022016 Ligase 1
12022074 10x Ligase Buffer 1
12022017 Dilution Buffer 1
12022018 Stop Solution 1
16012872 10x PLA Splint 1
10042051 Dnase 1
12022020 10x Dnase Buffer 1
12007991 ddPCR Supermix for Probes No dUTP 0.7ML 2
Catalog Number Description Quantity
12022087 Vericheck ddPCR Empty Full Capsid Kit AAV2 Box 1
12022076 AAV2 Antibody 1 1
12022019 AAV2 Antibody 2 1
12022376 AAV2 Positive Control 1

Instrument Compatibility

Instrument Catalog Number
QX ONE Droplet Digital PCR System 12006536
QX200 AutoDG Droplet Digital PCR System consisting of:
QX200 Droplet Reader
Automated Droplet Generator
1864100
1864003
1864101
QX600 Droplet Digital PCR Reader 12013328
QX Manager Software, Regulatory Edition 12012172
QX ONE Software, Regulatory Edition 12012078
QX Manager, Premium Edition 12018108

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