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Overview
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 1,000 x 20 µl reactions
Safety Data Sheet
Description
Description
Use SsoFast EvaGreen Supermix with Low ROX for fast, highly specific amplification. This supermix is designed for use with real-time PCR systems that have an excitation source and optical configuration that require lower levels of ROX. EvaGreen has similar spectral properties to SYBR® Green I and fluorescein.
Key Features and Benefits
- EvaGreen has lower levels of PCR inhibition than SYBR® Green I, and can be used at higher concentrations to generate stronger signals
- Patented Sso7d fusion polymerase* has a dsDNA-binding domain that stabilizes the polymerase- template complex, increasing processivity and reducing reaction times
- Antibody-mediated hot start increases specificity since the polymerase is not active until after the initial denaturation
Applications and Uses
- Dye-based qPCR detection
- PCR and real-time PCR analysis
- Nucleic acid amplification and expression profiling
- Gene expression analysis
- Genotyping studies
- Genomics-related applications
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
| 200 | 2 x 1 | 1725210 |
| 500 | 5 x 1 | 1725211** |
| 1,000 | 10 x 1 | 1725212** |
| 2,000 | 1 x 20 | 1725213** |
**Not available in Japan
See the SsoFast EvaGreen Supermix with Low ROX product page for more information and documentation.
Related Products
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Supporting Documents
- SsoFast EvaGreen Supermix with Low ROX Product Insert
- Amplification Plastics and Reagents Selection Guide
Online Resources
*U.S. patent 7,560,260.