Overview
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, ROX passive reference dye, stabilizers, for 1,000 x 20 µl reactions
Available Safety Data Sheets
Safety Data Sheet
Find Certificate of Analysis
Description
Description
Use SsoFast EvaGreen Supermix with Low ROX for fast, highly specific amplification. This supermix is designed for use with real-time PCR systems that have an excitation source and optical configuration that require lower levels of ROX. EvaGreen has similar spectral properties to SYBR® Green I and fluorescein.
Key Features and Benefits
- EvaGreen has lower levels of PCR inhibition than SYBR® Green I, and can be used at higher concentrations to generate stronger signals
- Patented Sso7d fusion polymerase* has a dsDNA-binding domain that stabilizes the polymerase- template complex, increasing processivity and reducing reaction times
- Antibody-mediated hot start increases specificity since the polymerase is not active until after the initial denaturation
Applications and Uses
- Dye-based qPCR detection
- PCR and real-time PCR analysis
- Nucleic acid amplification and expression profiling
- Gene expression analysis
- Genotyping studies
- Genomics-related applications
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
| 200 | 2 x 1 | 1725210 |
| 500 | 5 x 1 | 1725211** |
| 1,000 | 10 x 1 | 1725212** |
| 2,000 | 1 x 20 | 1725213** |
**Not available in Japan
See the SsoFast EvaGreen Supermix with Low ROX product page for more information and documentation.
Related Products
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Supporting Documents
- SsoFast EvaGreen Supermix with Low ROX Product Insert
- Amplification Plastics and Reagents Selection Guide
Online Resources
*U.S. patent 7,560,260.