Running Buffers and Reagents

Running Buffers and Reagents

Premixed buffers are available for a variety of protein and nucleic acid electrophoresis protocols. The high quality 0.4 µm filtered buffers can be used with handcast or precast gels.

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Overview

Reduce preparation time and eliminate a source of error by using Bio-Rad’s range of premixed protein electrophoresis running buffers. There are no reagents to weigh or filter; just dilute with distilled deionized water. A wide variety of premixed Tris buffers (such as Tris/glycine/SDS, Tris/glycine, and Tris/tricine/SDS) and high-purity, electrophoresis-grade powders (such as Tris, glycine, tricine, and urea) are available for protein electrophoresis and can be used with both handcast gels and Bio-Rad’s precast gels (see the protein gel selection guide).

Features and Benefits of Running Buffers and Reagents for Protein Electrophoresis

  • Premixed buffers – all electrophoresis-purity reagents used are quality controlled for reproducible results; save preparation time while avoiding potential error due to incorrect buffer concentration
  • Variety of sizes – electrophoresis running buffers and reagents are available in packages ranging from 250 ml bottles to 5 L cubes for different throughput needs
  • Bulk 5 L boxes offer economy and convenience; the boxes feature easy-pouring spouts and are compact and stackable to save bench space

Applications and Uses of Running Buffers and Reagents for Protein Electrophoresis

Bio-Rad’s high-quality premixed electrophoresis running buffers are used for

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Compare protein electrophoresis buffers to select the most appropriate buffers for your workflow.

To choose the best gel for your application, see the gel selection guide. Choices include the innovative TGX™ SDS-PAGE system with precast Mini-Protean® TGX™ and Mini-Protean® TGX Stain-Free™ Gels, and midi-sized Criterion™ TGX™ and Criterion™ TGX Stain-Free™ Gels. For hand casting, TGX™ FastCast™ and TGX Stain-Free™ FastCast™ Acrylamide Solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels.

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(Discontinued) 10x Tris/Glycine/SDS
1610732
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) 10x Tris/Glycine/SDS
1610772
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
1610734

Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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(Discontinued) 10x Tris/Glycine Buffer for Western Blots and Native Gels
1610771

Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

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(Discontinued) 10x Tris/Tricine/SDS Running Buffer, 1 L
1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) 10x IEF Cathode Buffer, 250 ml
1610762

Pkg of 1, 250 ml, electrophoresis buffer, 20 mM lysine, 20 mM arginine, for use in isoelectric focusing

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(Discontinued) 20x XT MOPS Running Buffer Kit
1610793
Pkg of 1, electrophoresis buffer kit, includes 500 ml XT MOPS Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml XT Reducing Agent
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(Discontinued) XT MES Buffer Kit
1610796
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT MES Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent
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(Discontinued) XT Tricine Buffer Kit
1610797
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent

Documents

Number Description Options
2317
Ready-to-Run Buffers and Solutions Brochure, Rev F