Macro-Prep® 25 S Cation Exchange Resin

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Macro-Prep 25 S Cation Exchange Resin

Overview

Specifications
  Macro-Prep® 25 Q Support Macro-Prep 25 S Support
Type of ion exchanger Strong anion Strong cation
Functional group –N+(CH3)3 –SO3-
Ionic capacity (µeq/ml) 220 ±40 110 ±30
Typical binding capacity >30 mg/ml BSA >40 mg/ml IgG
Shipping counterion Cl- Na+
Nominal particle size 25 µm 25 µm
Nominal pore size 725 Å 725 Å
Max linear flow rate 3,000 cm/hr 3,000 cm/hr
Chemical stability:
   1% SDS, 24hr

   6M guanidine-HCI, 24 hr

Yes

Yes

Yes

Yes
Volume changes:
   pH 4–10

   0.1–1.0 M NaCl

<1%

<5%

<1%

<4%
Autoclavable
(121°C, 30 min)
Yes Yes
pH stability 1–10 1–10
Antimicrobial agent 20% ethanol 20% ethanol
Product Selection by Technique
   
Scale
Packaging Format
Characteristics Product Type Analytical Pilot/Preparative Process Prepacked Bottled
Ion Exchange Chromatography
Separation by charge UNOsphere™ media     UNOsphere Q, S, Rapid S
General biomolecules purification Macro-Prep® media     Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications Macro-Prep 25 media     Macro-Prep 25 Q, 25 S
UNO® columns   UNO Q, S  
Bio-Scale™ Mini Cartridges     UNOsphere Q, S  
High capacity         Macro-Prep High Q, S  
Good capture technique AG® resins   AG
Chelex® resins Chelex
Bio-Rex® resins Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique selectivity CHT™ 80 µm support     CHT, Types I and II
Alternative to protein A for antibody purification CHT 40 µm support     CHT, Types I and II
CHT 20 µm support   CHT, Types I and II
CFT 40 µm support CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA Bio-Gel® HT and HTP media     Bio-Gel HT, HTP
Bio-Scale columns   CHT Type I (10 µm)  
CHT Type II (20 µm)  
Affinity Chromatography
Purification of His- and GST- tagged proteins Profinity™ IMAC and GST media   Bio-Scale Mini Profinity IMAC
Separation by biological interaction Affi-Gel resins     Affi-Gel® Protein A*
Affi-Gel Blue*
DEAE Affi-Gel Blue*
CM Affi-Gel Blue*
High selectivity           Affi-Gel boronate, heparin**
High purity in a single step           Affi-Gel Hz, 10, 15**
Affi-Gel 102**
Excellent capture technique Profinity epoxide media     Profinity epoxide**
Affi-Prep media   Affi-Prep protein A*
Affi-Prep polymyxin*
Bio-Scale Mini cartridges   Profinity IMAC and GST  
Econo-Pac® columns   Affi-Gel Blue  
DEAE Affi-Gel Blue
Affi-Gel protein A
Size Exclusion Chromatography
Separation by size Bio-Gel P media     Bio-Gel P
MW determination Bio-Beads™ S-X media     Bio-Beads S-X
Applicable to all antibody purifications Bio-Sil® and Bio-Silect™ columns     Bio-Sil, Bio-Silect  
Desalting or buffer exchange Bio-Scale Mini cartridges     Bio-Gel P-6  
High resolution Econo-Pac columns     Econo-Pac 10DG  
Hydrophobic Interaction Chromatography
Separation by hydrophobicity Macro-Prep HIC media     Macro-Prep t-butly
Macro-Prep methyl

* Ready to use.
** Activated.

Common Buffers for Ion Exchange
Type of Ion Exchanger Buffer Buffering Range
Macro-Prep 25 S Acetic acid 4.8–5.2
  Citric acid 4.2–5.2
  HEPES 7.6–8.2
  Lactic acid 3.6–4.3
  MES 5.5–6.7
  MOPS 6.5–7.9
  Phosphate 6.7–7.6
  PIPES 6.1–7.5
  Pivalic acid 4.7–5.4
  TES 7.2–7.8
  Tricine 7.8–8.9
Macro-Prep 25 Q Bicine 7.6–9.0
  Bis-Tris 5.8–7.2
  Diethanolamine 8.4–8.8
  Diethylamine 9.5–11.5
  L-histidine 5.5–6.0
  Imidazole 6.6–7.1
  Pyridine 4.9–5.6
  Tricine 7.4–8.8
  Triethanolamine 7.3–8.3
  Tris 7.5–8.0
Linear Flow Rate vs. Operating Pressure

The Macro-Prep 25 S support was evaluated for linear flow rate vs. operating pressure by measuring the pressure at a series of flow rates using 1M NaCl on a 1.6 cm (ID) x 12.5 cm (H) column. The pressure plotted was determined by subtracting the pressure generated by the column when empty from the pressure generated from the column when packed.

Separation on Macro-Prep 25 S
Separation of a mixture of 1 mg of native protein from 0.23 mg of the protein with oxidized surface methione residues from treatment with t-butyl hydroperoxide on a 1.6 cm (ID) x 12.5 cm (L) column packed with Macro-Prep 25 S.Buffer A: 50 mM KH2PO4, pH 5.0. Buffer B: 50mM KH2PO4, 2 M KCl, pH 5.5; gradient: 50% B to 100 % B in 20 min; flow rate: 2 ml/min.
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Macro-Prep 25 S strong cation exchange support offers high-resolution separations at high flow rates with medium pressures. It possessesthe same rigid, macroporous, and hydrophilic properties of 50 μm Macro-Prep High Q and High S resin, but in a 25 μm bead that offers higher resolution. Benefits include:

  • Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
  • Fewer required chromatographic steps
  • Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation
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Macro-Prep 25 S Support

1530031
50 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

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Macro-Prep 25 S Support

1530032
200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

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(Discontinued) Macro-Prep 25 S Support

153-0033
1 L, strong cation exchange chromatography media, 25 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) Macro-Prep 25 S Support

153-0034
5 L, strong cation exchange chromatography media, 25 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
4006129 Instruction Manual, Macro-Prep 25 Ion Exchange Resins, Ver C Click to download

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