iQ-Check STEC PCR Detection Kits 

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IQ-Check STEC VirX Kit

Overview

Key Benefits

Faster Results

  • Test results within hours after 10 hr enrichment
  • Rapid virulence gene screening of up to 94 samples

Easy To Use

  • Easy one step DNA extraction
  • Automated amplification and detection
  • Automatic result interpretation

Minimal Risk

  • Kits developed to comply with USDA FSIS testing requirements
  • Positive and negative PCR controls, plus an internal amplification control
  • Specific primers and probes

A complete Solution

  • Standardized Bio-Rad reagents:
  • Enrichment media
  • iQ-Check kits
  • Chromogenic agars
  • Validated Bio-Rad equipment and software for automated data analysis
Protocol

Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


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Open in new window
Open in new window
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Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Complete method to detect non-O157 STEC virulence genes and Big-6 serogroups and E. coli O157:H7 in raw beef meats, raw milk products, produce, and environmental samples. Next-day results.


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Ordering Information


  • Description
  • Ordering
  • Accessories
  • Equipment
  • Documents

Description

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The iQ-Check STEC (Shiga toxin-producing E. coli) PCR Detection Kits consists of two test kits, iQ-Check STEC VirX and iQ-Check STEC SerO, based on the fast, sensitive, and proven technology of real-time PCR.

The kits are intended for the detection of STEC virulence genes and 7 major STEC serogroups in raw beef meats, raw milk products, produce and environmental samples.

The iQ-Check STEC VirX Kit allows the separate detection of the stx1-stx2 and eae virulence genes, in a single reaction, according the ISO TS 13136 or the USDA FSIS MLG5B.02 standard methods. If the results come out positive, the same DNA extract can be used to for the detection of the “Big Six” STEC (O26, O111, O45, O145, O121, and O103) as well as E. coli O157:H7 using the iQ-Check STEC SerO Kit.

Due to the sensitivity and specificity of PCR, detection of the virulence genes can be obtained in only few hours following microbial enrichment. The enrichment is as short as 10 hr for raw meat samples, and up to 15 hr for other samples.

Highly Specific and Reliable

The iQ-Check STEC kits use an optimized system of primers and patented probes to ensure high specificity. Both kits are multiplex kits and allow the detection of several targets in a single well, including an internal positive amplification control, which validates any negative result.

Maximum Flexibility

Developed as a flexible solution, iQ-Check STEC VirX Kits can be used for up to 94 samples and iQ-Check STEC SerO Kit for up to 30 samples. The two kits can be run in parallel, on Bio-Rad’s high-throughput and multiplex real-time PCR systems.

Related Products

Bio-Rad offers a complete solution including:

  • STEC enrichment media (3564001)
  • Modified Tryptic Soy Broth (mTSB) (3564426)
  • Real-time PCR kits

Ordering

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iQ-Check STEC VirX PCR Detection Kit

3578139
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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iQ-Check STEC SerO PCR Detection Kit

3578140
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 32 samples

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iQ-Check Free DNA Removal Solution

3594970
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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$570.00 
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iQ-Check Purification Reagent

12012383
Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests

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$103.00 
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Modified Tryptic Soy Broth (mTSB) Base, dehydrated

3564426
Pkg of 1, 500 g of dehydrated base

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$102.00 
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STEC Enrichment Broth, dehydrated

3564001
Pkg of 1, 500 g of dehydrated broth

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$103.00 
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STEC Enrichment Broth, dehydrated

3564002
Pkg of 1, 5 kg of dehydrated broth

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$960.00 
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Selective STEC Supplement

3564005
Selective STEC Supplement, vial 1 g (235 tests)

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$355.00 
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Buffered Peptone Water Plus

3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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$28.00 
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Buffered Peptone Water Plus

3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

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$113.00 
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Buffered Peptone Water Plus

3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

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$114.00 
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Buffered Peptone Water Plus

3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

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$78.00 
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Buffered Peptone Water Plus

3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

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$716.00 
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Buffered Peptone Water Standard

12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions

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$99.00 
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Buffered Peptone Water Standard, dehydrated

12013259
Pkg of 1, 500 g — for standard enrichment conditions

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$68.00 
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Buffered Peptone Water Standard, dehydrated

12013258
Pkg of 1, 5 kg — for standard enrichment conditions

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$622.00 
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Accessories

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
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Microseal 'B' PCR Plate Sealing Film, adhesive, optical

MSB1001
Pkg of 100, optically clear seal for PCR plates

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$191.00 
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X-Pierce Films

3593977
Pkg of 100, PCR plate sealing film, pre-pierced

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$189.00 
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1.5 ml Conical Tubes, screwcap, sterile

2240110XTU
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

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$157.00 
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PCR Tube Strips without Caps, low-profile, 0.2 ml, 8-tube strips

TLS0851XTU
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)

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$130.00 
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PCR Tube 8-Cap Strips, optical, flat, 8 caps

TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)

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$43.00 
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Multiplate™ 48-Well PCR Plates, low-profile, unskirted, white

MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples

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$163.00 
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Multiplate 96-Well PCR Plates, low-profile, unskirted

MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

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$141.00 
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Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white

HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

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$298.00 
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Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/white, barcoded

HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

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$304.00 
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Strip Cap Tool for PCR Plates and PCR Tubes

ECT2000
Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes

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$46.00 
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iQ-Check Deep Well Microplates

3594900
Pkg of 50, 96-well plates plates, deep well

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$285.00 
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Equipment

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(Discontinued) Heating Thermoshaker

3594999
Pkg of 1, temperature-controlled shaker for cell lysis, for use with iQ-Check PCR Detection Kits

This product is no longer available. Please contact technical support for more information on replacement product.
CFX Manager Software, Industrial Diagnostic Edition (IDE)

3593893
1 unit

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$3,178.00 
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CFX96 Touch Deep Well Real-Time PCR Detection System

3600037
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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iQ-Check Prep System

3594911
Automated system for DNA extraction and real-time PCR plate set-up, 1 unit

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Documents

Number Description Options
FSD_16439 iQ-Check Real-Time PCR Solution Brochure Click to download
FSD_808475 iQ-Check STEC SerO Kit User Guide, Rev D Click to download
FSD_808474 iQ-Check STEC VirX Kit User Guide, Rev C Click to download
7163 iQ-Check iQ-Check STEC VirX kit Quick Guide: Easy I and Easy II Deep Well Protocols Using the Thermoshaker Click to download
6960 iQ-Check Prep Solution Brochure Click to download
FSD_200 iQ-Check STEC SerO Kit Quick Guide: Using the CFX Manager IDE Software Click to download
FSD_194 iQ-Check STEC VirX Kit Quick Guide: Standard II Extraction Deepwell Protocol – Raw Beef Samples, Rev B Click to download
PF_243 iQ-Check® Stec VirX Kit Quick Guide: Easy I Extraction Deepwell Protocol – Raw Milk Products, Produce, and Environmental Samples, Rev A Click to download
10000058391 iQ-Check Free DNA Removal Solution Product Insert Click to download
7322 iQ-Check Free DNA Removal Solution — Quick Guide Click to download
6834 iQ-Check Free DNA Removal Solution Brochure Click to download
10000119734 iQ-Check Purification Reagent Technical sheet Click to download
FSD_17933 Food Safety and Quality Reference Guide Click to download
7157EN TS Selective STEC Supplement Click to download
7274 Complete Solutions for Service Laboratories Click to download
10000128740 Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
10000128738 Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download

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