iQ-Check STEC PCR Detection Kits
Complete method to detect non-O157 STEC virulence genes and Big-6 serogroups and E. coli O157:H7 in raw beef meats, raw milk products, produce, and environmental samples. Next-day results.
Overview
The iQ-Check STEC (Shiga toxin-producing E. coli) PCR Detection Kits consists of two test kits, iQ-Check STEC VirX and iQ-Check STEC SerO, based on the fast, sensitive, and proven technology of real-time PCR.
The kits are intended for the detection of STEC virulence genes and 7 major STEC serogroups in raw beef meats, raw milk products, produce and environmental samples.
The iQ-Check STEC VirX Kit allows the separate detection of the stx1-stx2 and eae virulence genes, in a single reaction, according the ISO TS 13136 or the USDA FSIS MLG5B.02 standard methods. If the results come out positive, the same DNA extract can be used to for the detection of the “Big Six” STEC (O26, O111, O45, O145, O121, and O103) as well as E. coli O157:H7 using the iQ-Check STEC SerO Kit.
Due to the sensitivity and specificity of PCR, detection of the virulence genes can be obtained in only few hours following microbial enrichment. The enrichment is as short as 10 hr for raw meat samples, and up to 15 hr for other samples.
Highly Specific and Reliable
The iQ-Check STEC kits use an optimized system of primers and patented probes to ensure high specificity. Both kits are multiplex kits and allow the detection of several targets in a single well, including an internal positive amplification control, which validates any negative result.
Maximum Flexibility
Developed as a flexible solution, iQ-Check STEC VirX Kits can be used for up to 94 samples and iQ-Check STEC SerO Kit for up to 30 samples. The two kits can be run in parallel, on Bio-Rad’s high-throughput and multiplex real-time PCR systems.
Related Products
Bio-Rad offers a complete solution including:
- STEC enrichment media (3564001)
- Modified Tryptic Soy Broth (mTSB) (3564426)
- Real-time PCR kits
- Real-time PCR detection systems, CFX96 Touch Real-Time PCR Detection System (3600037) including software for automated analysis
Read the press release: MicroVal Approves Bio-Rad PCR Kit for Detection of STEC in Raw Meat and Dairy Products
Ordering
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Pkg of 1 kit, includes fluorescent probes, PCR amplification mix, positive and negative controls, for 32 samples
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)
Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests
Selective STEC Supplement, vial 1 g (235 tests)
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
Accessories
Pkg of 100, optically clear seal for PCR plates for real-time PCR
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps
Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only
Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas
Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only
Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only
Equipment
CFX Manager Software, Industrial Diagnostic Edition
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.
96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables
96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables
