The iQ-Check STEC (Shiga toxin-producing E. coli) PCR Detection Kits consists of two test kits, iQ-Check STEC VirX and iQ-Check STEC SerO, based on the fast, sensitive, and proven technology of real-time PCR.
The kits are intended for the detection of STEC virulence genes and 7 major STEC serogroups in raw beef meats, raw milk products, produce and environmental samples.
The iQ-Check STEC VirX Kit allows the separate detection of the stx1-stx2 and eae virulence genes, in a single reaction, according the ISO TS 13136 or the USDA FSIS MLG5B.02 standard methods. If the results come out positive, the same DNA extract can be used to for the detection of the “Big Six” STEC (O26, O111, O45, O145, O121, and O103) as well as E. coli O157:H7 using the iQ-Check STEC SerO Kit.
Due to the sensitivity and specificity of PCR, detection of the virulence genes can be obtained in only few hours following microbial enrichment. The enrichment is as short as 10 hr for raw meat samples, and up to 15 hr for other samples.
Highly Specific and Reliable
The iQ-Check STEC kits use an optimized system of primers and patented probes to ensure high specificity. Both kits are multiplex kits and allow the detection of several targets in a single well, including an internal positive amplification control, which validates any negative result.
Developed as a flexible solution, iQ-Check STEC VirX Kits can be used for up to 94 samples and iQ-Check STEC SerO Kit for up to 30 samples. The two kits can be run in parallel, on Bio-Rad’s high-throughput and multiplex real-time PCR systems.
Bio-Rad offers a complete solution including:
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Pkg of 1 kit, includes fluorescent probes, PCR amplification mix, positive and negative controls, for 32 samples
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.