iQ-Check STEC PCR Detection Kits 

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Overview

Complete method to detect non-O157 STEC virulence genes and Big-6 serogroups and E. coli O157:H7 in raw beef meats, raw milk products, produce, and environmental samples. Next-day results.

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Description

Description

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The iQ-Check STEC (Shiga toxin-producing E. coli) PCR Detection Kits consists of two test kits, iQ-Check STEC VirX and iQ-Check STEC SerO, based on the fast, sensitive, and proven technology of real-time PCR.

The kits are intended for the detection of STEC virulence genes and 7 major STEC serogroups in raw beef meats, raw milk products, produce and environmental samples.

The iQ-Check STEC VirX Kit allows the separate detection of the stx1-stx2 and eae virulence genes, in a single reaction, according the ISO TS 13136 or the USDA FSIS MLG5B.02 standard methods. If the results come out positive, the same DNA extract can be used to for the detection of the “Big Six” STEC (O26, O111, O45, O145, O121, and O103) as well as E. coli O157:H7 using the iQ-Check STEC SerO Kit.

Due to the sensitivity and specificity of PCR, detection of the virulence genes can be obtained in only few hours following microbial enrichment. The enrichment is as short as 10 hr for raw meat samples, and up to 15 hr for other samples.

Highly Specific and Reliable

The iQ-Check STEC kits use an optimized system of primers and patented probes to ensure high specificity. Both kits are multiplex kits and allow the detection of several targets in a single well, including an internal positive amplification control, which validates any negative result.

Maximum Flexibility

Developed as a flexible solution, iQ-Check STEC VirX Kits can be used for up to 94 samples and iQ-Check STEC SerO Kit for up to 30 samples. The two kits can be run in parallel, on Bio-Rad’s high-throughput and multiplex real-time PCR systems.

Related Products

Bio-Rad offers a complete solution including:

  • STEC enrichment media (3564001)
  • Modified Tryptic Soy Broth (mTSB) (3564426)
  • Real-time PCR kits

Ordering

Ordering

items

Use the filters below to refine results!

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iQ-Check STEC SerO II PCR Detection Kit
12013174

Pkg of 1 kit, includes fluorescent probes, PCR amplification mix, positive and negative controls, for 32 samples

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3578139

Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

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3564426
Pkg of 1, 500 g of dehydrated base
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3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
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3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Accessories

Accessories

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TLS0851XTU

Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)

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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Documents

Documents

Number Description Options
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
3311
CFX Opus 96 Real-Time PCR System brochure
3309
CFX Opus 96 and CFX Opus Deepwell Real-Time PCR system product information sheet
10000125262
iQ-Check STEC SerO II PCR Detection Kit, User Guide (English, French, German, Italian, Portuguese, Spanish)
7445
iQ-Check STEC SerO II Kit Quick Guide: Using CFX Manager IDE Software
10000131516
iQ-Check STEC VirX, User Guide (English, French, German, Italian, Portuguese, Spanish)
3213
Performance summary, iQ-Check STEC VirX and SerO
7163
iQ-Check iQ-Check STEC VirX kit Quick Guide: Easy I and Easy II Deep Well Protocols Using the Thermoshaker
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
6960
iQ-Check Prep Solution Brochure
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
FSD_17933
Food Safety and Quality Reference Guide
10000141057
Selective STEC Supplement, User Guide (English, French, German, Italian, Portuguese, Spanish)
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish, Chinese)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)
3276
CFX Manager Software (IDE) iQ-Check Prep sample setup Protocol, Quick Guide