iQ-Check STEC PCR Detection Kits 

iQ-Check STEC PCR Detection Kits 

Complete method to detect non-O157 STEC virulence genes and Big-6 serogroups and E. coli O157:H7 in raw beef meats, raw milk products, produce, and environmental samples. Next-day results.

Read More
Ordering Information

Overview

The iQ-Check STEC (Shiga toxin-producing E. coli) PCR Detection Kits consists of two test kits, iQ-Check STEC VirX and iQ-Check STEC SerO, based on the fast, sensitive, and proven technology of real-time PCR.

The kits are intended for the detection of STEC virulence genes and 7 major STEC serogroups in raw beef meats, raw milk products, produce and environmental samples.

The iQ-Check STEC VirX Kit allows the separate detection of the stx1-stx2 and eae virulence genes, in a single reaction, according the ISO TS 13136 or the USDA FSIS MLG5B.02 standard methods. If the results come out positive, the same DNA extract can be used to for the detection of the “Big Six” STEC (O26, O111, O45, O145, O121, and O103) as well as E. coli O157:H7 using the iQ-Check STEC SerO Kit.

Due to the sensitivity and specificity of PCR, detection of the virulence genes can be obtained in only few hours following microbial enrichment. The enrichment is as short as 10 hr for raw meat samples, and up to 15 hr for other samples.

Highly Specific and Reliable

The iQ-Check STEC kits use an optimized system of primers and patented probes to ensure high specificity. Both kits are multiplex kits and allow the detection of several targets in a single well, including an internal positive amplification control, which validates any negative result.

Maximum Flexibility

Developed as a flexible solution, iQ-Check STEC VirX Kits can be used for up to 94 samples and iQ-Check STEC SerO Kit for up to 30 samples. The two kits can be run in parallel, on Bio-Rad’s high-throughput and multiplex real-time PCR systems.

Related Products

Bio-Rad offers a complete solution including:

  • STEC enrichment media (3564001)
  • Modified Tryptic Soy Broth (mTSB) (3564426)
  • Real-time PCR kits

Read the press release: MicroVal Approves Bio-Rad PCR Kit for Detection of STEC in Raw Meat and Dairy Products

Ordering

items

Use the filters below to refine results!

Image
iQ-Check STEC SerO II PCR Detection Kit
12013174

Pkg of 1 kit, includes fluorescent probes, PCR amplification mix, positive and negative controls, for 32 samples

Image
iQ-Check_Listeria_spp _2019_SKU_TN.jpg
3578139

Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

Image
iQ-Check-Free-DNA-Removal-16-0708-thumb.jpg
3594970

Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

Image
356-4426_160.jpg
3564426
Pkg of 1, 500 g of dehydrated base
Image
355-4179_160px.jpg
3554179

Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

Image
356-4684_160.jpg
3564684

Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

Accessories

Image
TCS-0803_tn.jpg
TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
Image
mll-4851_tn.jpg
MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
Image
mll-9651_tn.jpg
MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
Image
HSP9655_tn.jpg
HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
Image
HSP9955_tn.jpg
HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design
Image
17010688
17010688

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Image
17010624
2112036XTU

Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNas

Image
17010692
2112021XTU

Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only

Image
17010691
2112006XTU

Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Equipment

Image
185-5096_tn.jpg
3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Image
12011319_tn.jpg
17007992

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

Image
12016658
17007991

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables

Documents

Number Description