Experion Pro260 Starter Kit

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Overview

The Experion Pro260 starter kit contains all materials necessary to get started with protein analysis on the Experion system with step-by-step instructions and tips for successful separations.
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Description

Description

The Experion™ Pro260 starter kit is intended for new or experienced users of the Experion automated electrophoresis system. This kit contains all materials necessary to get started on the Experion system, allowing the user to practice techniques and analyze data or validate the system using a known protein standard (bovine IgG). Get started or practice with step-by-step instructions and tips for successful microfluidic separations using the provided standard before moving onto your own samples. An Experion RNA starter kit is also available for studying RNA samples (StdSens RNA starter kit).

The Experion protein starter kit includes all the necessary consumables to illustrate the utility of the Experion system in protein applications. This kit provides information on:

  • How best to prepare and load a protein chip
  • Protein quantitation and sizing using a known standard
  • Creating and running a standard curve for protein analysis
  • Tips and common mistakes

The Pro260 protein starter kit contains:

  • Experion Pro260 reagents
  • Experion Pro260 chips, 3
  • Narrow-bore polypropylene pipet tips
  • Polypropylene microcentrifuge tubes
  • 0.2 µ filtered water
  • DTT reducing agent
  • Spin filters
  • Electrode cleaner
  • Cleaning swabs
  • Cleaning chips
  • Bovine IgG control sample
  • Detailed instruction manual

The Experion automated electrophoresis system can also be used for a number of upstream and downstream workflows in other nucleic acid and protein applications.

Specifications

Specifications

Component
Storage Condition 
Bovine γ-globulin (BGG) standard (2.0 mg/ml), 2 ml
4°C 
Dithiothreitol (DTT)
4°C  
DEPC-treated water 1, 50 ml
Room temperature 
Experion™ electrode cleaner, 25 ml
Room temperature  
Experion cleaning chip, 1
Room temperature   
Cleaning swabs, 25
Room temperature    
Narrow-bore 20 µl pipet tips, 96 (1 rack)
Room temperature     
0.65 ml RNase-free microcentrifuge tubes, 50
Room temperature      
Experion Pro260 chips, 3
Room temperature       
Reagents for analysis
 
Pro260 gel, 2 vials, 520 µl
4°C   
Pro260 ladder, 60 µl
4°C    
Pro260 sample buffer, 400 µl
4°C     
Pro260 stain, 45 µl
4°C      
Spin filters
4°C       
Experion training video: Chip Loading (DVD)
Instructions

Ordering

Ordering

items

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7007110
Starter kit for Experion protein analysis, includes 3 Pro260 chips, reagents, spin filters, IgG protein standard, DTT, cleaning supplies, tips, tubes, ultrapure water
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7007256
60 μl, protein size standards, replacement component of Experion Pro260 analysis kits
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7007151
Pkg of 10 plus 1 cleaning chip, microfluidic chips for protein analysis with the Experion automated electrophoresis system
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7007251
Pkg of 10, cleaning chip for use with the Experion electrophoresis station (#700-7010)
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7007252
250 ml, for use with Experion cleaning chips (#700-7251)
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7007254
Pkg of 10, spin filter, for use with Experion analysis kits
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(Discontinued) Cleaning Swabs
7007264
Pkg of 25, swab for use with any Experion analysis kit

Kits & Reagents

Kits & Reagents

Documents

Documents

Number Description Options
3140
Experion Automated Electrophoresis System Brochure, Rev F
3169
Experion Pro260 Analysis Kit PIS, Rev E
3171
Experion Software PIS, Rev C
3176
Monitoring the Expression, Purification, and Processing of GST-Tagged Proteins Using the Experion Automated Electrophoresis System, Rev A
3186
Experion Automated Electrophoresis System Flier, Rev A
5253
Experion System Performance Comparison, Rev A
5299
Performance Comparison of the Experion Automated Electrophoresis System and SDS-PAGE for Protein Analysis, Rev B
5302
Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
5328
Experion Automated Electrophoresis System and the Experion Pro260 Analysis Kit: Accurate and Reproducible Protein Sizing and Quantitation in the Presence of High Salt Concentrations, Rev A
5350
Monitoring the Expression, Processing, and Purification of GST-Tagged Proteins Using the Experion Automated Electrophoresis System (Poster Version), Rev A
5351
Rapid and Accurate Protein Sizing, Quantitation, and Analysis Using the Experion Automated Electrophoresis System and the Experion Pro260 Analysis Kit (Poster), Rev A
5355
Rapid Screening of Mini Rotofor Fractions Using the Experion Automated Electrophoresis System (Poster), Rev A
5423
Protein Quantitation: a Comparative Analysis Using the Experion Automated Electrophoresis System, Bradford and Modified Lowry Assays, and SDS-PAGE, Rev A
5453
Application of the Experion Automated Electrophoresis System to Glycoprotein Visualization and Analysis, Rev A
6959
Biomarker Workflow Solutions Brochure, Rev A
5501
Rapid, Efficient Purification and Evaluation of His-Tagged Proteins, Rev A
5506
Monitoring Development of Chromatographic Methods With the Experion Automated Electrophoresis System, Rev B
5514
Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A
5712
The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A
5732
Experion Pro260 and RNA StdSens Starter Kits PIS, Rev B
5770
Affinity Purification of Native, Tag-Free Cystathionine Beta-Lysate (MetC) From Shewanella oneidensis Using the Profinity eXact™ Fusion-Tag and Profinia™ Protein Purification Systems, Rev A
5783
Experion Automated Electrophoresis System Flier, Rev A
5784
Comparison of Protein Quantitation Methods Using the Experion Automated Electrophoresis System
5868
Performance Comparison of Two LabChip Microfluidic Platforms for Protein, DNA, and RNA Analyses, Rev A
5926
Modular Protein Purification, Rev A
10000975
Instruction Manual, Experion Pro260 Analysis Kit, Rev C
10001312
Instruction Manual, Experion Automated Electrophoresis System, Rev C
10004490
Quick Guide, Experion Pro260 Analysis Kit, Rev C
10005250
Instruction Manual, IQ/OQ Protocols and Instuctions, Experion Electrophoresis Station, Rev D
10005581
Instruction Manual, Experion Security Edition Software Setup Guide, Rev C
10005740
Quick Installation Guide, Experion System Software Windows XP, Rev A
10010510
Instruction Manual, Experion™ Pro260 Starter Kit, Rev A
10010511
Instruction Manual, Experion RNA StdSens Starter Kit, Rev A
10014627
Quick Installation Guide, Experion System Software Windows VISTA, Rev A