CFX Opus Real-Time PCR Systems

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CFX Opus Real-Time PCR Systems

Overview

Choose the CFX Opus Real-Time PCR System for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab.
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Description

Choose the CFX Opus Real-time PCR System for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab.

The CFX Opus System is the next evolution in qPCR from Bio-Rad. With improved thermal performance and our proprietary, accurate optical shuttle system, your data will be more consistent than ever. A sleek, modern design includes a refreshed and easy-to-use interface with new connectivity options and features.

In addition to ethernet compatibility, the CFX Opus System can connect directly to Wi-Fi for completely wireless operation and data retrieval. The CFX Opus System is capable of stand-alone operation and direct data transfer to a USB device, or it can be connected to a computer.

With internet connectivity, the CFX Opus System can be programmed to send your data and notifications directly to your email or local networked drive, enabling easy access by collaborators or from other network-connected locations.

Features and Benefits

  • Quick system set up — easy installation and factory-calibrated optics
  • Our best thermal uniformity and accuracy to date
  • Offers Wi-Fi connectivity and BR.io cloud platform analysis tools
  • Sensitive modern touch screen — responsive even with double gloves
  • Updated interface is easier to use and more intuitive than ever
  • Uniform optical system free of regular calibration requirements
  • Audit and e-signature compatibility with CFX Maestro Security Edition Software
  • Available in 96-well (5-color detection) and 384-well (4-color detection) formats

Applications and Uses

  • Gene expression without requiring ROX
  • End-point analysis for sequence detection
  • Protein thermal shift assays
  • Assay optimization with thermal gradient

Related Products

CFX Maestro Software Works Seamlessly with PrimePCR Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

  • Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
  • Combine data from multiple plates into a gene study to rapidly screen large numbers of targets or samples
  • Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments


Specifications

Thermal Cycler
Maximum ramp rate, °C/sec CFX Opus 96 System: 5
CFX Opus 384 System: 2.5
Average ramp rate, °C/sec CFX Opus 96 System: 3.3
CFX Opus 384 System: 2
Heating and cooling method Peltier
Lid, °C 30–110
Temperature
Range, °C 4–100
Accuracy, °C ±0.2 of programmed target at 90°C
Uniformity, °C ±0.3 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C 30–100
Programmable span, °C 1–24
Optical Detection
Excitation CFX Opus 96 System: 6 filtered LEDs
CFX Opus 384 System: 5 filtered LEDs
Detection CFX Opus 96 System: 6 filtered photodiodes
CFX Opus 384 System: 5 filtered photodiodes
Range of excitation wavelengths, nm CFX Opus 96 System: 450–684
CFX Opus 384 System: 450–650
Range of emission wavelengths, nm CFX Opus 96 System: 510–730
CFX Opus 384 System: 515–690
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Software
Operating systems Windows 10
System
Sample capacity, wells 96-well and 384-well versions
Sample size, µl CFX Opus 96 System: 1–50 (10–50 recommended)
CFX Opus 384 System: 1–30 (5–30 recommended)
Communication interface USB 2.0
Electrical approvals IEC, CE
Dimensions (W x D x H), cm/in 33x56x36 / 13x22x14
Weight, kg/lb 22/48
Touch Screen Adjustable from 15°–22°
Embedded Operating System Windows 10 IoT

Documents

Number Description Options
7279 CFX Opus Real-Time PCR System Brochure Click to download
7280 CFX Opus Real-Time PCR System Research Flier Click to download
7281 CFX Opus Real-Time PCR System BioPharma Flier Click to download
10000119983 CFX Opus Instrument Guide Click to download
10000119983 CFX Opus Instrument Guide Click to download
10000126764 CFX Maestro Software User Guide Click to download
10000124722 CFX Opus 96 and CFX Opus 384 Real-Time PCR Systems Quick Start Guide Click to download
7299 CFX Opus 96 Real-Time PCR system Product Information Sheet Click to download
7300 CFX Opus 384 Real-Time PCR system Product Information Sheet Click to download

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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