Overview
The CFX Duet Real-Time PCR System can analyze 96 wells and offers:
- Quick system set up — easy installation and factory-calibrated optics
- Our best thermal uniformity and accuracy to date
- Uniform optical system free of regular calibration requirements
- No need for a reference/normalizing dye
- Can perform duplex assays, including genotyping
- Connects to a PC running CFX Maestro Software (version 2.3 or higher, sold separately)
Learn more on the CFX Duet Real-Time PCR System product page.
Specifications
Thermal Cycler
Maximum ramp rate, °C/sec
5
Average ramp rate, °C/sec
3.3
Lid, °C
30–110
Temperature
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.3 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
Excitation
2 filtered LEDs
Detection
3 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–580
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Scan Time
All channels, sec
12
FAM/SYBR® Green only, sec
3
Software
Operating systems
Windows 10 and up
Multiplex analysis
Up to 2 targets per well
System
Sample capacity, wells
96
Sample size, µl
1–50 (10–50 recommended)
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x D x H), cm/in
33x56x36 / 13x22x14
Weight, kg/lb
22/48
Documents
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Recommended Products
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(Discontinued) CFX Maestro 2.3 Software for Windows PC
12013758EDU
1 license, CFX Maestro 2.3, software for real-time PCR plate setup, data collection, statistics, and graphing of results; for Windows PCs; for educational use only