Cell Lysis RT-qPCR Kits

Cell Lysis RT-qPCR Kits

SingleShot Cell Lysis RT-qPCR Kits allow RT-qPCR to be performed directly from cultured cells without RNA purification. Kits are available for both one-step and two-step RT-qPCR with SYBR Green and probe chemist

Read More
Ordering Information

Overview

Single Shot Workflow

SingleShot Cell Lysis RT-qPCR Kits provide a complete and fast solution for the generation of lysates from cell cultures, optimized for downstream one step or two step quantitative PCR (qPCR) reactions, without an RNA purification step.

Kits are available in SYBR Green or probe chemistries. A cell lysis kit is also offered as a stand-alone option.

All kits offer the following key benefits:

Choose the Right Kit for Your Needs

SingleShot Kit Features Product Options
Cell Lysis One-Step RT-qPCR Kits
  • Cell lysis, reverse transcription, and qPCR
  • All one-step RT-qPCR reagents included
  • No RNA purification needed
SYBR Green
100 reactions (1725095)
Probes
100 reactions (1725070)
Cell Lysis Two-Step RT-qPCR Kits
  • Cell lysis, reverse transcription, and qPCR
  • All two-step RT-qPCR reagents included
  • No RNA purification needed
SYBR Green
100 reactions (1725085)
Probes
100 reactions (1725090)
Cell Lysis Kits 100 reactions (1725080)
500 reactions (1725081)

Simple Workflow

Simple, short, minimal hands-on protocol is suitable for automation and provides a high-throughput solution.

Grow cells to desired density, transfer up to 105 cells to wells of a PCR plate, add lysis solution, and incubate at room temperature for 10 minutes.

Heat for 5 minutes at 37°C and then for 5 minutes at 75°C. The lysate is ready for RT-qPCR or can be stored for future use.

Preamp Single Shot Workflow

Cell Lines Validated for Use with the SingleShot Cell Lysis RT-qPCR Kits

All SingleShot Cell Lysis RT-qPCR Kits have been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line Property Species
A549 Adherent H. sapiens, lung carcinoma
HeLa Adherent H. sapiens, cervical adenocarcinoma
HepG2 Adherent H. sapiens, liver carcinoma
NIH/3T3 Adherent M. musculus, embryonic fibroblast
MCF-7 Adherent  H. sapiens, epithelial, adenocarcinoma
NCI-H1975 Adherent H. sapiens, lung adenocarcinoma 
K-562 Suspension H. sapiens, chronic myelogenous leukemia
HEK 293 Suspension H. sapiens, kidney
Jurkat Suspension H. sapiens, T lymphocyte
PC-12 Suspension R. norvegicus, pheochromocytoma, adrenal
HepRG Primary-like H. sapiens, hepatocytes

Related Categories

 

Supporting Documentation

Online Resources

Specifications

Storage at 4°C
6 months:
Lysis buffer

3 months:
iTaq™ Universal One-Step Reaction Mix
SsoAdvanced™ Universal Supermix
Storage at –20°C
All kit components are stable for 12 months

Ordering

items

Use the filters below to refine results!

Image
single-shot-cell-lysis-kit-172-5080-thumb.jpg
1725080

Pkg of 1, kit includes 5 ml (1 x 5 ml) of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions.

Image
single-shot-cell-lysis-kit-172-5081-thumb.jpg
1725081

Pkg of 1, kit includes 25 ml (5 x 5 ml) of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions

Image
singleshot-sybr-green-kit-172-5085-thumb.jpg
1725085

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

Image
single-shot-probes-kit-172-5090-thumb.jpg
1725090

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal Probes Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

Image
singleshot-sybr-green-one-step-kit-172-5095-thumb.jpg
1725095

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal SYBR® Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

Image
singleshot-probes-one-step-kit-172-5070-thumb.jpg
1725070

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal Probes One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

Documents

Number Description
[ Add to Cart (Free) ]