SingleShot™ SYBR® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions #1725095

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Overview

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal SYBR® Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions
Safety Data Sheet

Safety Data Sheet

Description

Description

Use the SingleShot™ SYBR® Green One-Step Kit to obtain high-quality RT-qPCR data with SYBR® Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.

Benefits include:

Optimized Sample Input

The SingleShot™ SYBR® Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:

Maximum cell lysate into RT-qPCR reaction 20% of total volume

Kit Contents

SingleShot Cell Lysis Reagents
SingleShot Cell Lysis Buffer
SingleShot Proteinase K Solution
SingleShot DNase Solution
100 x 50 μl reactions
5 ml (1 x 5 ml vial)
100 μl (1 x 1 ml vial)
100 μl (1 x 1 ml vial)

iTaq™ Universal SYBR® Green One-Step Kit
iScript™ Reverse Transcriptase
2x iTaq™ Universal SYBR® Green One-Step Reaction Mix
100 reactions
25 μl (1 x 1 ml vial)
1 ml (1 x 1 ml vial)

SingleShot RNA Control
SingleShot RNA Control Template
Singleshot™ SYBR® Green qPCR Control Assay
200 reactions
Lyophilized (200 reactions)
200 μl (1 x 1 ml vial)
 

Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot™ SYBR® Green One-Step Kit

The SingleShot™ SYBR® Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line Property Species
A549 Adherent  H. sapiens, lung carcinoma
HeLa Adherent H. sapiens, cervical adenocarcinoma
HepG2   Adherent  H. sapiens, liver carcinoma
NIH/3T3 Adherent M. musculus, embryonic fibroblast
MCF-7 Adherent  H. sapiens, epithelial, adenocarcinoma
NCI-H1975  Adherent H. sapiens, lung adenocarcinoma 
K-562 Suspension H. sapiens, chronic myelogenous leukemia
HEK 293 Suspension H. sapiens, kidney
Jurkat Suspension H. sapiens, T lymphocyte
PC-12  Suspension R. norvegicus, pheochromocytoma, adrenal
HepRG Primary-like H. sapiens, hepatocytes
 

Related Products

The SingleShot™ SYBR® Green One-Step Kit belongs to the family of SingleShot™ Cell Lysis RT-pPCR Kits:

  • SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit (172-5095) and SingleShot Probes One-Step Kit (172-5070)
  • SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit (172-5085) and SingleShot Probes Kit (172-5090)
  • SingleShot Cell Lysis Kit — available for 100 reactions (172-5080)

Specifications

Specifications

Storage at –20°C
12 months:
Lysis buffer, DNase, and proteinase K solution
iTaq™ Universal SYBR® Green One-Step Reaction Mix
iScript™ Reverse Transcriptase
SingleShot RNA Control Template
SingleShot™ SYBR® Green qPCR Control Assay
Storage at 4°C
6 months:
Lysis buffer

Up to 3 months:
iTaq™ Universal SYBR® Green One-Step Reaction Mix