SingleShot™ SYBR® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions #1725095

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1725095 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [3.88.220.93, 10.232.18.13] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/1725095-singleshot-sybr-green-one-step-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions] [x-amzn-trace-id] = [Root=1-5d0b8d05-a77c515b6f96cffb5cb6db8a] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1725095] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-us/sku/1725095-singleshot-sybr-green-one-step-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1725095-singleshot-sybr-green-one-step-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions LSR en US en-us /prd 07/30/13 03:25 AM en_US true internet/generic 2019-05-04 20:39:39 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1522700291361 2019-05-04 20:39:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the high school or college level, visit our <a href="/en-us/education/education-discount-policy?ID=MRFKWO4VY">Education Discount Policy</a> page to establish an education account number.</p> </div> /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 1725095 06/04/14 06:51 PM en_US true internet/product_SKU 2019-05-04 16:19:23 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml /default/main/bio-rad/WORKAREA/default jcrucyx2 2017-11-14 07:07:36 true jcrucyx2 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml LSR N /default/main/bio-rad en 06/04/24 07:07 PM 1555974590912 2019-05-04 16:19:23 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml 1725095-singleshot-sybr-green-one-step-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions 1 1725095 SingleShotSYBRGreenOne-StepKit SingleShot™ SYBR<sup>®</sup> Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/singleshot-sybr-green-one-step-kit-172-5095-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/singleshot-sybr-green-one-step-kit-172-5095.jpg preview ,VN Karen Moss SingleShot&trade; SYBR&reg; Green One-Step Kit SingleShot™ SYBR<sup>®</sup> Green One-Step Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions 1725095, singleshot, sybr, green, one-step, itaq, universal, one, step, cell, lysis, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 06:51 PM 06/04/24 07:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit </strong>to obtain high-quality RT-qPCR data with SYBR<sup>&reg;</sup> Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.</p> <p>Benefits include:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html');">linear dynamic range</a> of qPCR data across genes</li> <li>Results comparable to using purified RNA, including <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html');">dynamic range</a></li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control ensures optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer <br /> SingleShot Proteinase K Solution <br /> SingleShot DNase Solution</td> <td width="172" align="left" valign="top"><strong>100 x 50 &mu;l reactions</strong><br /> 5 ml (1 x 5 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit </strong><br /> iScript&trade; Reverse Transcriptase<br /> 2x iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix</td> <td width="172" align="left" valign="top"><strong> 100 reactions</strong><br /> 25 &mu;l (1 x 1 ml vial)<br /> 1 ml (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot RNA Control </strong><br /> SingleShot RNA Control Template <br />Singleshot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay</td> <td width="172" align="left" valign="top"><strong> 200 reactions</strong><br /> Lyophilized (200 reactions)<br /> 200 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> &nbsp; <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042475.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">Related Products</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit belongs to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-pPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/genomic-dna-removal-from-cell-lysates.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#reproducibility').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/reproducibility-of-technical-lysate.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-probes-one-step-kit-172-5095-desc1.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-probes-one-step-kit-172-5095-desc1.html Storage at –20°C 2199 <strong>12 months:</strong><br /> Lysis buffer, DNase, and proteinase K solution<br /> iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix<br /> iScript&trade; Reverse Transcriptase <br /> SingleShot RNA Control Template<br /> SingleShot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer<br /><br /><strong>Up to 3 months:</strong><br />iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&CartQuote=QT&quantity= 1450 false true false $1,241.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&AddToDefaultWishList=true&quantity= true false true 12161500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&quantity= 172-5095-MSDS SingleShot™ One-Step SYBR Green Kit Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_1725095.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_1725095.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_1725095.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_1725095.pdf Mexico Spanish /webroot/web/pdf/WWMSDS/LSGC/MEX/MEX_ES_1725095.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_1725095.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_1725095.pdf Russian Federation Russian /webroot/web/pdf/WWMSDS/LSGC/RUS/RUS_RUS_1725095.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_1725095.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_1725095.pdf Brazil Portuguese /webroot/web/pdf/WWMSDS/LSGC/BRA/BRA_PT_1725095.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_1725095.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_1725095.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_1725095.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_1725095.pdf Turkey Turkish /webroot/web/pdf/WWMSDS/LSGC/TR/TR_TR_1725095.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_1725095.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_1725095.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_1725095.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_1725095.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_1725095.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_1725095.pdf Ireland English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_1725095.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_1725095.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_1725095.pdf true China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_1725095.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_1725095.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_1725095.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_1725095.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_1725095.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_1725095.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_1725095.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_1725095.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_1725095.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_1725095.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_1725095.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_1725095.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_1725095.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_1725095.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_1725095.pdf Finland Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_1725095.pdf Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_1725095.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_1725095.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_1725095.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_1725095.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_1725095.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_1725095.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_1725095.pdf

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal SYBR® Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

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To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the high school or college level, visit our <a href="/en-us/education/education-discount-policy?ID=MRFKWO4VY">Education Discount Policy</a> page to establish an education account number.</p> </div> /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 1725095 06/04/14 06:51 PM en_US true internet/product_SKU 2019-05-04 16:19:23 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml /default/main/bio-rad/WORKAREA/default jcrucyx2 2017-11-14 07:07:36 true jcrucyx2 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml LSR N /default/main/bio-rad en 06/04/24 07:07 PM 1555974590912 2019-05-04 16:19:23 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml 1725095-singleshot-sybr-green-one-step-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions 1 1725095 SingleShotSYBRGreenOne-StepKit SingleShot™ SYBR<sup>®</sup> Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/singleshot-sybr-green-one-step-kit-172-5095-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/singleshot-sybr-green-one-step-kit-172-5095.jpg preview ,VN Karen Moss SingleShot&trade; SYBR&reg; Green One-Step Kit SingleShot™ SYBR<sup>®</sup> Green One-Step Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions 1725095, singleshot, sybr, green, one-step, itaq, universal, one, step, cell, lysis, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 06:51 PM 06/04/24 07:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit </strong>to obtain high-quality RT-qPCR data with SYBR<sup>&reg;</sup> Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.</p> <p>Benefits include:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html');">linear dynamic range</a> of qPCR data across genes</li> <li>Results comparable to using purified RNA, including <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html');">dynamic range</a></li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control ensures optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer <br /> SingleShot Proteinase K Solution <br /> SingleShot DNase Solution</td> <td width="172" align="left" valign="top"><strong>100 x 50 &mu;l reactions</strong><br /> 5 ml (1 x 5 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit </strong><br /> iScript&trade; Reverse Transcriptase<br /> 2x iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix</td> <td width="172" align="left" valign="top"><strong> 100 reactions</strong><br /> 25 &mu;l (1 x 1 ml vial)<br /> 1 ml (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot RNA Control </strong><br /> SingleShot RNA Control Template <br />Singleshot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay</td> <td width="172" align="left" valign="top"><strong> 200 reactions</strong><br /> Lyophilized (200 reactions)<br /> 200 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> &nbsp; <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042475.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">Related Products</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit belongs to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-pPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/genomic-dna-removal-from-cell-lysates.html'); 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Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix<br /> iScript&trade; Reverse Transcriptase <br /> SingleShot RNA Control Template<br /> SingleShot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer<br /><br /><strong>Up to 3 months:</strong><br />iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&CartQuote=QT&quantity= 1450 false true false $1,241.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&AddToDefaultWishList=true&quantity= true false true 12161500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&quantity= 172-5095-MSDS SingleShot™ One-Step SYBR Green Kit Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_1725095.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_1725095.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_1725095.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_1725095.pdf Mexico Spanish /webroot/web/pdf/WWMSDS/LSGC/MEX/MEX_ES_1725095.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_1725095.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_1725095.pdf Russian Federation Russian /webroot/web/pdf/WWMSDS/LSGC/RUS/RUS_RUS_1725095.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_1725095.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_1725095.pdf Brazil Portuguese /webroot/web/pdf/WWMSDS/LSGC/BRA/BRA_PT_1725095.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_1725095.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_1725095.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_1725095.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_1725095.pdf Turkey Turkish /webroot/web/pdf/WWMSDS/LSGC/TR/TR_TR_1725095.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_1725095.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_1725095.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_1725095.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_1725095.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_1725095.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_1725095.pdf Ireland English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_1725095.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_1725095.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_1725095.pdf true China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_1725095.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_1725095.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_1725095.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_1725095.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_1725095.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_1725095.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_1725095.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_1725095.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_1725095.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_1725095.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_1725095.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_1725095.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_1725095.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_1725095.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_1725095.pdf Finland Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_1725095.pdf Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_1725095.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_1725095.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_1725095.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_1725095.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_1725095.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_1725095.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_1725095.pdf
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Use the SingleShot™ SYBR® Green One-Step Kit to obtain high-quality RT-qPCR data with SYBR® Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.

Benefits include:

Optimized Sample Input

The SingleShot™ SYBR® Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:

Maximum cell lysate into RT-qPCR reaction 20% of total volume

Kit Contents

SingleShot Cell Lysis Reagents
SingleShot Cell Lysis Buffer
SingleShot Proteinase K Solution
SingleShot DNase Solution
100 x 50 μl reactions
5 ml (1 x 5 ml vial)
100 μl (1 x 1 ml vial)
100 μl (1 x 1 ml vial)

iTaq™ Universal SYBR® Green One-Step Kit
iScript™ Reverse Transcriptase
2x iTaq™ Universal SYBR® Green One-Step Reaction Mix
100 reactions
25 μl (1 x 1 ml vial)
1 ml (1 x 1 ml vial)

SingleShot RNA Control
SingleShot RNA Control Template
Singleshot™ SYBR® Green qPCR Control Assay
200 reactions
Lyophilized (200 reactions)
200 μl (1 x 1 ml vial)
 

Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot™ SYBR® Green One-Step Kit

The SingleShot™ SYBR® Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line Property Species
A549 Adherent  H. sapiens, lung carcinoma
HeLa Adherent H. sapiens, cervical adenocarcinoma
HepG2   Adherent  H. sapiens, liver carcinoma
NIH/3T3 Adherent M. musculus, embryonic fibroblast
MCF-7 Adherent  H. sapiens, epithelial, adenocarcinoma
NCI-H1975  Adherent H. sapiens, lung adenocarcinoma 
K-562 Suspension H. sapiens, chronic myelogenous leukemia
HEK 293 Suspension H. sapiens, kidney
Jurkat Suspension H. sapiens, T lymphocyte
PC-12  Suspension R. norvegicus, pheochromocytoma, adrenal
HepRG Primary-like H. sapiens, hepatocytes
 

Related Products

The SingleShot™ SYBR® Green One-Step Kit belongs to the family of SingleShot™ Cell Lysis RT-pPCR Kits:

  • SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit (172-5095) and SingleShot Probes One-Step Kit (172-5070)
  • SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit (172-5085) and SingleShot Probes Kit (172-5090)
  • SingleShot Cell Lysis Kit — available for 100 reactions (172-5080)
Storage at –20°C 12 months:
Lysis buffer, DNase, and proteinase K solution
iTaq™ Universal SYBR® Green One-Step Reaction Mix
iScript™ Reverse Transcriptase
SingleShot RNA Control Template
SingleShot™ SYBR® Green qPCR Control Assay
Storage at 4°C 6 months:
Lysis buffer

Up to 3 months:
iTaq™ Universal SYBR® Green One-Step Reaction Mix
Number Description Options
Available Translation
SingleShot™ One-Step SYBR Green Kit  (SDS)gene

Bulletin# 172-5095-MSDS

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