Description
Description
Use the SingleShot Probes One-Step Kit to obtain high-quality RT-qPCR data with probe chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.
Benefits include:
- Cell lysate from 10 to 100,000 cells ready in 20 minutes
- Complete removal of genomic DNA while preserving RNA integrity
- Optimal accuracy and linear dynamic range of qPCR data across genes
- Results comparable to using purified RNA, including dynamic range
- Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr)
- SingleShot RNA Control ensures optimal cell and lysate inputs
Optimized Sample Input
The SingleShot Probes One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:
Maximum cell lysate into RT-qPCR reaction | 20% of total volume |
Kit Contents
SingleShot Cell Lysis Reagents SingleShot Cell Lysis Buffer SingleShot Proteinase K Solution SingleShot DNase Solution |
100 x 50 μl reactions 5 ml (1 x 5 ml vial) 100 μl (1 x 1 ml vial) 100 μl (1 x 1 ml vial) |
iTaq Universal Probes One-Step Kit iScript™ Reverse Transcriptase 2x iTaq Universal Probes One-Step Reaction Mix |
100 reactions 50 μl (1 x 1 ml vial) 1 ml (1 x 1 ml vial) |
SingleShot RNA Control SingleShot RNA Control Template SingleShot Probes qPCR Control Assay |
200 reactions Lyophilized (200 reactions) 200 μl (1 x 1 ml vial) |
Refer to the user manual for details on reaction setup and troubleshooting tips.
Cell Lines Validated for Use with the SingleShot Probes One-Step Kit
The SingleShot Probes One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:
Cell Line | Property | Species |
A549 | Adherent | H. sapiens, lung carcinoma |
HeLa | Adherent | H. sapiens, cervical adenocarcinoma |
HepG2 | Adherent | H. sapiens, liver carcinoma |
NIH/3T3 | Adherent | M. musculus, embryonic fibroblast |
MCF-7 | Adherent | H. sapiens, epithelial, adenocarcinoma |
NCI-H1975 | Adherent | H. sapiens, lung adenocarcinoma |
K-562 | Suspension | H. sapiens, chronic myelogenous leukemia |
HEK 293 | Suspension | H. sapiens, kidney |
Jurkat | Suspension | H. sapiens, T lymphocyte |
PC-12 | Suspension | R. norvegicus, pheochromocytoma, adrenal |
HepRG | Primary-like | H. sapiens, hepatocytes |
Related Products
The SingleShot Probes One-Step Kit belongs to the family of SingleShot™ Cell Lysis RT-qPCR Kits:
- SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit (172-5095) and SingleShot Probes One-Step Kit (172-5070)
- SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit (172-5085) and SingleShot Probes Kit (172-5090)
- SingleShot Cell Lysis Kit — available for 100 reactions (172-5080)
Specifications
Specifications
Lysis buffer, DNase, and proteinase K solution
iTaq Universal Probes One-Step Reaction Mix
iScript™ Reverse Transcriptase
SingleShot RNA Control Template
SingleShot Probes qPCR Control Assay
Lysis buffer
Up to 3 months:
iTaq Universal Probes One-Step Reaction Mix