AL (Agar Listeria according to Ottaviani and Agosti medium) Agar

Image
Food_Chromo_AL_pdp.jpg

Overview

Selective chromogenic medium used for the detection and enumeration of Listeria monocytogenes in food and environmental samples.
Read full description Ordering Information

Description

Description

Talk with a Specialist

AL Medium (Agar Listeria according to Ottaviani and Agosti) is based on the simultaneous detection of two enzyme activities: β-glucosidase and phosphatidylinositol-specific phospholipase C (PI-PLC).

Key Benefits

  • Detection of Listeria spp. in 24 hr after 24 hr of enrichment
  • Only1 plate needed per sample
  • Easy-to-read chromogenic reaction: blue colonies are Listeria spp., very easy to read on the whitish plate
  • Highly specific: tested on 50 strains of Listeria spp. and 30 strains of non-Listeria species
  • Certified NF VALIDATION according to the ISO 16140 standard

Principle

β-D-glucosidase activity, common to the Listeria genus, is detected using a chromogenic substrate (X-glucoside). Its hydrolysis induces the formation of a blue to blue-green color in all Listeria colonies.

PI-PLC is an enzyme only detected in pathogenic Listeria species: L. monocytogenes and L. ivanovii. AL medium contains phosphatidylinositol which, when it breaks down, produces an opaque halo around the colonies of these two bacterial species. The halo is visible after 24 hr for L. monocytogenes and 48 hr for L. ivanovii.

AL (Agar Listeria according to Ottaviani and Agosti) Chromogenic Assay

Enzymatic Activity L. monocytogenes Listeria spp. Non-Listeria
Pl-PLC dectection + - -
β-D-Glucosidase + + -

Ordering Information

Ordering

Ordering

items

Use the filters below to refine results!

356-3695_tn.jpg
3563695
Pkg of 20, 90 mm agar plates
3564043
Pkg of 1, 500 g of dehydrated broth, enough for 7.2 L of medium
3564041
Pkg of 10, vials of supplement 1, enough for 10 x 500 ml of medium
3564042
Pkg of 10, vials of supplement 2, enough for 10 x 500 ml of medium
355-5797_160px.jpg
3555797
Pkg of 6, 225 ml bottles
356-4604_160.jpg
3564604
Pkg of 1, 500 g of dehydrated broth base
356-4616_160.jpg
3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium
3555794
Pkg of 4, bags of medium, enough for 4 x 3 L
iQ-Check_Listeria_spp _2019_SKU_TN.jpg
3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
iQ-Check_Listeria_spp _2019_SKU_TN.jpg
3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
355-4179_160px.jpg
3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
3555790_th.jpg
3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions
3555795_th.jpg
3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions
356-4684_160.jpg
3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
3564686_th.jpg
3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions
3555795_th.jpg
12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions
12013259_th.jpg
12013259
Pkg of 1, 500 g — for standard enrichment conditions
12013258_th.jpg
12013258
Pkg of 1, 5 kg — for standard enrichment conditions

Documents

Documents

Number Description Options
10000135677
AL Agar, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)