AL (Agar Listeria according to Ottaviani and Agosti medium) Agar

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AL (Agar Listeria according to Ottaviani and Agosti Medium)

Overview

Key Benefits
  • Economical: 1 broth + 1 plate
  • Fast: 24-hour results after enrichment
  • Easy to read: blue colony with/without halo
  • Flexible: detection, enumeration or ISO 11290-1 and -2 standard
  • Reliable: NF validation (ISO 16140)


Protocol
Detection of Campylobacter spp.
Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


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Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Selective chromogenic medium used for the detection and enumeration of Listeria monocytogenes in food and environmental samples.
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Ordering Information


Description

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AL Medium (Agar Listeria according to Ottaviani and Agosti) is based on the simultaneous detection of two enzyme activities: β-glucosidase and phosphatidylinositol-specific phospholipase C (PI-PLC).

Key Benefits

  • Detection of Listeria spp. in 24 hr after 24 hr of enrichment
  • Only1 plate needed per sample
  • Easy-to-read chromogenic reaction: blue colonies are Listeria spp., very easy to read on the whitish plate
  • Highly specific: tested on 50 strains of Listeria spp. and 30 strains of non-Listeria species
  • Certified NF VALIDATION according to the ISO 16140 standard

Principle

β-D-glucosidase activity, common to the Listeria genus, is detected using a chromogenic substrate (X-glucoside). Its hydrolysis induces the formation of a blue to blue-green color in all Listeria colonies.

PI-PLC is an enzyme only detected in pathogenic Listeria species: L. monocytogenes and L. ivanovii. AL medium contains phosphatidylinositol which, when it breaks down, produces an opaque halo around the colonies of these two bacterial species. The halo is visible after 24 hr for L. monocytogenes and 48 hr for L. ivanovii.

AL (Agar Listeria according to Ottaviani and Agosti) Chromogenic Assay

Enzymatic Activity L. monocytogenes Listeria spp. Non-Listeria
Pl-PLC dectection + - -
β-D-Glucosidase + + -

Ordering Information


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AL (Agar Listeria according to Ottaviani and Agosti) Agar Plates

3563695
Pkg of 20, 90 mm agar plates

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$91.00 
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AL (Agar Listeria according to Ottaviani and Agosti) Agar Base, dehydrated

3564043
Pkg of 1, 500 g of dehydrated broth, enough for 7.2 L of medium

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$569.00 
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Hazardous  
AL (Agar Listeria according to Ottaviani and Agosti) Agar; Supplement 1

3564041
Pkg of 10, vials of supplement 1, enough for 10 x 500 ml of medium

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$148.00 
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AL (Agar Listeria according to Ottaviani and Agosti) Agar with Supplement 2

3564042
Pkg of 10, vials of supplement 2, enough for 10 x 500 ml of medium

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$330.00 
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AL (Agar Listeria according to Ottaviani and Agosti) Agar Base, ready to use

3555200
Pkg of 6, 250 ml bottle

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$90.00 
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Fraser 1/2 Broth, ready to use

3555797
Pkg of 6, 225 ml bottles

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$44.00 
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Fraser Broth Base, dehydrated

3564604
Pkg of 1, 500 g of dehydrated broth base

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$117.00 
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Fraser 1/2 Strength Supplement

3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium

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$203.00 
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Fraser 1/2 Broth, ready to use

3555794
Pkg of 4, bags of medium, enough for 4 x 3 L

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$223.00 
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iQ-Check Listeria spp. PCR Detection Kit

3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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iQ-Check Listeria monocytogenes II PCR Detection Kit

3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Buffered Peptone Water Plus

3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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$28.00 
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Buffered Peptone Water Plus

3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

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$113.00 
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Buffered Peptone Water Plus

3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

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$114.00 
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Buffered Peptone Water Plus

3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

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$78.00 
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Buffered Peptone Water Plus

3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

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$716.00 
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Buffered Peptone Water Standard

12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions

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$99.00 
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Buffered Peptone Water Standard, dehydrated

12013259
Pkg of 1, 500 g — for standard enrichment conditions

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$68.00 
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Buffered Peptone Water Standard, dehydrated

12013258
Pkg of 1, 5 kg — for standard enrichment conditions

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$622.00 
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Documents

Number Description Options
10000135677 AL Agar, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
FSD_16288 RAPID’Chromogenic Media Brochure Click to download
FSD_17933 Food Safety and Quality Reference Guide Click to download
7274 Complete Solutions for Service Laboratories Click to download
10000128740 Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
10000128738 Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download

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