DEAE Affi-Gel Blue Gel

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Overview

DEAE Affi-Gel Blue Gel is a bifunctional affinity/ion exchange chromatography matrix prepared by coupling Cibacron blue F3GA and diethylaminoethyl groups to Bio-Gel A.
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Description

Description

DEAE Affi-Gel Blue Gel is a bifunctional affinity/ion exchange chromatography matrix prepared by coupling Cibacron Blue F3GA and diethylaminoethyl groups to Bio-Gel A Gel. The Cibacron blue F3GA functions as an ionic, hydrophobic, or sterically active binding site for proteins with dinucleotide folds, such as albumin. The diethylaminoethyl functional group acts as an anion exchanger and binds proteins with isoelectric points higher than the pH of the mobile phase. This bifunctionality allows you to obtain highly purified IgG from serum from a wide variety of species through careful optimization of the ionic strength and pH of the application buffer.

Features and Benefits

  • Single-step IgG purification from serum; the eluted IgG contains a small amount of transferrin (samples are diluted approximately 5-fold)
  • Albumin removal
  • No detectable proteolytic activity in the eluted IgG fraction
  • Economical alternative to protein A affinity chromatography

More Information

Instruction Manual, DEAE Affi-Gel Blue Support (Bulletin 9191)

Ordering

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1537307
100 ml, ready-to-use bifunctional affinity gel with Cibacron Blue F3GA dye covalently coupled to DEAE Bio-Gel A

Kits & Reagents

Kits & Reagents

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7322010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit
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7322027
Antibody purification kit, includes 5 x 5 ml DEAE Affi-Gel Blue columns, 1 x 10 ml 10DG column, buffers
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7324634
Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324632
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324651
Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue