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      DEAE Affi-Gel Blue Gel

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      Overview

      DEAE Affi-Gel Blue Gel is a bifunctional affinity/ion exchange chromatography matrix prepared by coupling Cibacron blue F3GA and diethylaminoethyl groups to Bio-Gel A.

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      Description

      Description

      DEAE Affi-Gel Blue Gel is a bifunctional affinity/ion exchange chromatography matrix prepared by coupling Cibacron Blue F3GA and diethylaminoethyl groups to Bio-Gel A Gel. The Cibacron blue F3GA functions as an ionic, hydrophobic, or sterically active binding site for proteins with dinucleotide folds, such as albumin. The diethylaminoethyl functional group acts as an anion exchanger and binds proteins with isoelectric points higher than the pH of the mobile phase. This bifunctionality allows you to obtain highly purified IgG from serum from a wide variety of species through careful optimization of the ionic strength and pH of the application buffer.

      Features and Benefits

      • Single-step IgG purification from serum; the eluted IgG contains a small amount of transferrin (samples are diluted approximately 5-fold)
      • Albumin removal
      • No detectable proteolytic activity in the eluted IgG fraction
      • Economical alternative to protein A affinity chromatography

      More Information

      Instruction Manual, DEAE Affi-Gel Blue Support (Bulletin 9191)

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      Kits & Reagents

      Kits & Reagents

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      (Discontinued) Econo-Pac 10DG Desalting Columns
      7322010
      Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit
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      (Discontinued) Bio-Scale Mini DEAE Affi-Gel Blue Cartridges
      7324634
      Pkg of 5, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
      The following replacement is recommended:
      • EconoFit DEAE Affi-Gel Blue Columns, 5 x 5 ml ( 12009263 )
      732-4632_tn.jpg
      (Discontinued) Bio-Scale Mini DEAE Affi-Gel Blue Cartridge
      7324632
      Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
      The following replacement is recommended:
      • EconoFit DEAE Affi-Gel Blue Column, 1 x 5 ml ( 12009262 )
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      (Discontinued) Bio-Scale Mini Affinity Sampler Pack
      7324651
      Includes one 1 ml cartridge each of IMAC and Affi-Prep protein A, one 5 ml cartridge each of DEAE Affi-Gel Blue and Affi-Gel Blue
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      (Discontinued) EconoFit DEAE Affi-Gel Blue Column, 1 x 5 ml
      12009262
      Pkg of 1, 5 ml, prepacked affinity column, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system
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      (Discontinued) EconoFit DEAE Affi-Gel Blue Columns, 5 x 5 ml
      12009263
      Pkg of 5, 5 ml, prepacked affinity columns, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system