ddPCR Supermix for Probes (No dUTP)

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ddpcr-supermix-for-probes-no-dUTP-detail.jpg

Overview

This digital PCR supermix delivers maximum PCR efficiency and sensitivity for the amplification and detection of DNA targets using hydrolysis probe–based assays.

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Description

Description

This digital PCR supermix for probes (No dUTP) is a 2x concentrated, ready-to-use universal mix that has been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital™ PCR (ddPCR™).

Note: This product was previously named droplet PCR supermix.

Key Benefits

  • Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Optimized for use with validated ddPCR Assays
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
  • Allows for DNA recovery after amplification

Applications and Uses

  • Absolute quantification
  • Mutation detection
  • Copy number analysis
  • Gene expression analysis
  • NGS validation and library quantification
  • Environmental monitoring
  • Food testing

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 186-3023
500 5 x 1 186-3024
2,500 5 x 5 186-3025

Additional Reagents

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

This ddPCR Supermix for Probes (No dUTP) is optimized for use with Droplet Generation Oil for Probes on the QX600 or QX200 Droplet Digital PCR System.

Specifications

Specifications

Storage at –20°C
Up to 18 months (refer to expiration date)
Storage at 4°C (after thawing)
Up to 2 weeks
Template compatibility
cDNA, genomic DNA, plasmid DNA

Ordering

Ordering

items

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1863023

2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems

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1863024

5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems

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1863025

25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems

Equipment

Equipment

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17007769

Discover how the QX600 Droplet Digital PCR System enables 6-color multiplexing, allowing clear discrimination of multiple targets within samples. 

Contact a Specialist

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17008371
17008371

Discover how the QX600 AutoDG Droplet Digital PCR System enables and automates 6-color multiplexing for up to 96-samples per run providing clear discrimination of multiple targets. 

Contact a Specialist

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12013328
(Discontinued) QX600 Droplet Reader
12013328

The QX600™ Droplet Reader enables advanced six-color multiplexing, allowing clear discrimination of multiple targets with assays that are cross-compatible with the QX200™ Droplet Digital™ PCR (ddPCR™) System. The QX600 Droplet Reader is designed for investigators who need to quantify multiple targets with high accuracy, reproducibility, and sensitivity. 

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1864101
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX200/QX100; Droplet Digital PCR Systems
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1864001

Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications

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1864002

Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System

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(Discontinued) PX1 PCR Plate Sealer
1814000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord
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1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

Accessories

Accessories

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1814040
Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

Documents

Documents

Number Description Options
6338
Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A
[ Add to Cart (Free) ]
10026868
ddPCR™ Supermix for Probes (No dUTP) Product Insert
10040718
Droplet Generation Oil for Probes Product Insert
6311
QX200 Droplet Digital PCR System Brochure, Rev C
[ Add to Cart (Free) ]
10031907
Instruction Manual, QX200™ Droplet Generator, Rev C
10031906
Instruction Manual, QX200 Droplet Reader and QuantaSoft Software
6712
Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital™ PCR Application Note, Ver B