Description
Description
This digital PCR supermix for probes (No dUTP) is a 2x concentrated, ready-to-use universal mix that has been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital™ PCR (ddPCR™).
Note: This product was previously named droplet PCR supermix.
Key Benefits
- Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Optimized for use with validated ddPCR Assays
- Hot-start polymerase keeps enzyme inactive during sample droplet partitioning
- Allows for DNA recovery after amplification
Applications and Uses
- Absolute quantification
- Mutation detection
- Copy number analysis
- Gene expression analysis
- NGS validation and library quantification
- Environmental monitoring
- Food testing
Packaging Options
| Number of 20 µl Reactions | Supermix Volume, ml | Catalog Number |
| 200 | 2 x 1 | 186-3023 |
| 500 | 5 x 1 | 186-3024 |
| 2,500 | 5 x 5 | 186-3025 |
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
- Digital PCR consumables
- PrimePCR ddPCR Assays
- Digital PCR Library Quantification Kits
Additional Equipment
This digital PCR supermix requires the use of the following instruments and accessories:
- QX600 Droplet Digital PCR System
- QX600 AutoDG Droplet Digital PCR System
- QX200 Droplet Generator
- QX200 Droplet Reader
- PX1 PCR Plate Sealer
- PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96 Deep–Well Reaction Module
Related Products
Bio-Rad offers additional digital PCR supermixes including:
More Information
This ddPCR Supermix for Probes (No dUTP) is optimized for use with Droplet Generation Oil for Probes on the QX600 or QX200 Droplet Digital PCR System.
Specifications
Specifications
Ordering
Ordering
items
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2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
Equipment
Equipment
Discover how the QX600 Droplet Digital PCR System enables 6-color multiplexing, allowing clear discrimination of multiple targets within samples.
Discover how the QX600 AutoDG Droplet Digital PCR System enables and automates 6-color multiplexing for up to 96-samples per run providing clear discrimination of multiple targets.
The QX600™ Droplet Reader enables advanced six-color multiplexing, allowing clear discrimination of multiple targets with assays that are cross-compatible with the QX200™ Droplet Digital™ PCR (ddPCR™) System. The QX600 Droplet Reader is designed for investigators who need to quantify multiple targets with high accuracy, reproducibility, and sensitivity.
AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX600/QX200 Droplet Digital PCR Systems
Droplet Digital PCR System, includes droplet generator, droplet reader, computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive
Accessories
Accessories
70 ml (10 x 7 ml), oil for use with droplet generator in the QX600/QX200 Droplet Digital™ PCR Systems
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
Pkg of 24, cartridges for use with the QX200 Droplet Generator
Pkg of 24, gaskets for use with QX200 Droplet Generator
5 pkg of 24 DG8 Cartridges, 5 pkg of 24 DG8 Gaskets, for use with the QX200 Droplet Generator
