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iScript cDNA Synthesis Kits | iScript Explore Kit | Resources | Applications & Technologies | Related Products |
Earn a free one-year service contract on your CFX System with purchase of Reagents
CFX Loyalty ProgramiScript™ Reverse Transcription Reagents feature an RNase H+ MMLV reverse transcriptase plus an RNase inhibitor for reliable yields. Choose the best cDNA synthesis kit for your application, with formulations optimized for ease, yield, flexibility, fidelity, or gDNA clearance.
iScript cDNA Synthesis Kits
 iScript Reverse Transcription Supermix for RT-qPCR |
 iScript gDNA Clear cDNA Synthesis Kit |
 iScript Advanced cDNA Synthesis Kit for RT-qPCR |
 iScript Select cDNA Synthesis Kit |
 iScript cDNA Synthesis Kit |
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| Features |
Minimum reaction
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Effective gDNA removal before reverse transcription |
Maximum RNA input for high cDNA yields |
Flexible priming options and high-fidelity |
Reliable value solution |
| Applications |
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| Total Input RNA Range | 1 µg–1 pg | 1 µg–1 pg | 7.5 µg–100 fg | 1 µg–1 pg | 1 µg–100 fg |
| Kit Format | 1 tube | 3 tubes | 2 tubes | 5 tubes | 2 tubes |
| Kit Contents |
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| Time to Produce cDNA | 26 min | 36 min | 21 min | 15–65 min | 26 min |
| RNase H+ Activity | ✓ | ✓ | ✓ | ✓ | ✓ |
| Documents | Manual |
iScript™ Explore One-Step RT and PreAmp Kit
- Reverse transcription and unbiased preamplification completed in a single step
- Highly sensitive target detection
- Effective genomic DNA clearance
Resources
Understanding Reverse Transcription
This interactive tutorial provides an introduction to reverse transcription basics, an explanation of how different reagents work, and a protocol for comparing the results with different reverse transcription reagents.
Reagent Comparison Guide for Real-Time PCR
(PDF 3 MB)
Amplification Reagents and Plastics Brochure
(PDF 6.1 MB)
iScript™ Reverse Transcription Kits Flier
(PDF 208 KB)
Videos
Applications & Technologies
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
What is Gene Expression Analysis?
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
lncRNA RT-qPCR Workflow
This innovative workflow enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems
Related Products
Real-Time PCR Supermixes and Kits
PCR and real-time PCR reagents with advanced enzyme engineering and formulations optimized for a wide variety of templates and target sequences
One-Step RT-qPCR from RNA
For robust reverse transcription and SYBR® Green or probe-based qPCR in one step on any instrument
Real-Time PCR Detection Systems
An automation-compatible, gradient-enabled thermal cycler with interchangeable optical reaction modules for singleplex or multiplex real-time PCR
Cell Lysis RT-qPCR Kits
Complete and fast solution for one- or two-step qPCR reactions directly from cell lysates with no RNA purification step
Thermal Cyclers for PCR
Personal units and flexible modular thermal cyclers provide low- to high-throughput capabilities for all your PCR amplification needs
Preamplification Supermix
Ready-to-use supermix for unbiased, target-specific preamplification of cDNA or gDNA using up to 100 qPCR assays
PCR Plastic Consumables
A large selection of thin-wall polypropylene PCR tubes, PCR plates, seals, and accessories precisely manufactured for various PCR applications
