Overview
Affi-Gel blue affinity gel is a beaded, crosslinked agarose gel with covalently attached Cibacron Blue F3GA dye. It contains >1.9 mg dye per ml of gel and has a typical albumin capacity of >11 mg/ml. Affi-Gel Blue gel purifies a large range of proteins from widely divergent origins. The blue dye functions as an ionic, hydrophobic, aromatic, or sterically active binding site in various applications.
Affi-Gel Blue is ideally suited for albumin removal (using 50–100 mesh) and enzyme purification (using 100–200 mesh). Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of the eluent buffers.
For more information, see Bulletin 1107.
Specifications
50–100 mesh
100–200 mesh
150–300 µm
75–150 µm
Ordering
1 L, 100–200 mesh, ready-to-use crosslinked agarose bead with covalently attached Cibacron Blue F3GA dye
Pkg of 1, 5 ml, prepacked affinity column, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system
Pkg of 5, 5 ml, prepacked affinity columns, 16 mm x 25 mm, max. pressure 10 psi, for use with NGC Systems or any chromatography system