Zeta-Probe GT Membranes

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Overview

Zeta-Probe GT (genomic DNA-tested) membranes are designed for sensitive genomic applications. They are high-tensile-strength cationized nylon membranes that carry a high-density quaternary amine charge.
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Description

Description

Discontinued Product

Zeta-Probe GT Membranes are being discontinued and are available as supplies last.
Our Zeta-Probe Membranes are recommended as an alternative product. View all available Zeta-Probe Membranes.

Zeta-Probe GT (genomic DNA-tested) Membranes are designed for sensitive genomic applications. Like the original Zeta-Probe Membranes, these are high-tensile-strength cationized nylon membranes that carry a high-density quaternary amine charge. These pliable, flexible membranes are resistant to shrinking, tearing, and becoming brittle during transfer, hybridization, or reprobing procedures.

Zeta-Probe GT Membranes bind nucleic acids independently of buffer pH, so they can be used in rapid alkaline Southern or northern blotting techniques as well as in traditional Southern blots. The membranes bind nucleic acids in low-ionic-strength buffers, making electrophoretic transfer of nucleic acids from agarose and acrylamide gels possible. Oligonucleotides as short as 6 bases will bind the membrane through spot application, and those at least 20 bases long will be retained after repetitive hybridization and washing steps.

Every lot of Zeta-Probe GT Membranes is functionally tested to ensure that 3 pg of target single-copy factor VIII human DNA can be detected in 5 µg total genomic DNA using a radiolabeled probe. The blots are produced in two different ways prior to hybridization: a standard high-salt (10x SSC) Southern transfer, and a 4 hour alkaline transfer (0.4 N NaOH). In the quality control procedure, 5 and 10 µg of digested human genomic DNA are probed with a factor VIII cDNA clone (single-copy gene probe). This corresponds to actual target sizes of only 1.5 and 3.0 pg of DNA, respectively, which are visualized in an overnight exposure. Bio-Rad quality control procedures also include a 3 day exposure to check for nonspecific background. Zeta-Probe GT Membranes are accompanied by a performance certificate that illustrates the lot-specific results and includes an outline of the experiments and a photograph of the original autoradiogram.

References

  1. Gatti RA et al. (1984). Multiple uses of Southern blots. Biotechniques 2, 148-155.
  2. Li JK et al. (1987). Rapid alkaline blot-transfer of viral dsRNAs. Anal Biochem 163, 210-218.

Ordering

Ordering

items

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1620190
Pkg of 15, 9 x 12 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups
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1620191
Pkg of 15, 10 x 15 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups
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1620192
The 15 x 15 cm Zeta-Probe GT Membrane is now discontinued, and the 15 x 15 cm Zeta-Probe Membrane is recommended as an alternative product.
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1620193
Pkg of 15, 15 x 20 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups
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1620194
Pkg of 15, 20 x 20 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups
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1620195
Pkg of 3, 20 x 20 cm, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups
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1620196
Pkg of 1 roll, 30 cm x 3.3 m, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups
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1620197
Pkg of 1 roll, 20 cm x 3.3 m, genomic DNA-tested, high-strength nylon membrane positively charged with quaternary amine groups
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1620208
The 7 x 10 cm Zeta-Probe GT Membrane is now discontinued, and the 10 x 15 cm Zeta-Probe Membrane is recommended as an alternative product.

Kits & Reagents

Kits & Reagents

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(Discontinued) Gel Drying Solution
1610752
1 L, gel drying solution for use with polyacrylamide gels
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1610781
1 L, detergent, for easy pipetting
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(Discontinued) CHAPS
1610460
1 g, CHAPS detergent powder
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1610418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution
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1610416
250 ml, 10% (w/v) sodium dodecyl sulfate (SDS) solution
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1610407
500 ml, Triton X-100 detergent
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1610301
100 g, sodium dodecyl sulfate (SDS) powder
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(Discontinued) Cleaning Concentrate
1610722
1 kg, 50x alkaline detergent for cleaning glass plates and other laboratory equipment

Documents

Documents

Number Description Options
1645
Zeta-Probe® GT Membranes Product Information Sheet