UNOsphere SUPrA Resin

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Overview

UNOsphere SUPrA affinity chromatography resins are designed for process-scale purification of monoclonal antibodies. These affinity resins are built on the proven UNOsphere matrix for predictable performance.

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Description

Description

UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material from animal origin.

The UNOsphere™ base bead is a macroporous polymeric bead that is designed for robust and scaleable process applications. The outstanding flow-pressure performance of UNOsphere chromatography resin allows their use in large-scale process applications.

UNOsphere SUPrA affinity chromatography supports come with full regulatory support and are backed by the support of Bio-Rad’s global application and development team.

If you are interested in ordering large-volume quantities, please contact your local Bio-Rad representative to discuss your requirements.

Specifications

Specifications

Composition
Highly cross-linked polymer
Particle size range
53–61 µm
Ligand
Recombinant protein A
Coupling chemistry
Epoxy
Working pH range
3–11
Dynamic binding capacity*
30 ±3 mg/ml at 150 cm/hr
25 ±2 mg/ml at 300 cm/hr
20 ±2 mg/ml at 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical stability
10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-in-place (CIP) solutions
6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range
100–600 cm/hr
Temperature stability
4–40°C
Delivery conditions
50% slurry in 20% ethanol
Storage conditions
4–8°C
* 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
 

Ordering

Ordering

items

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156-0220
500 ml, protein A affinity chromatography media
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156-0221
5 L, protein A affinity chromatography media
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156-0222
10 L, protein A affinity chromatography media
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12009321
Pkg of 1, 1 ml, prepacked UNOsphere SUPrA Affinity Column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system
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12009322
Pkg of 5, 1 ml, prepacked UNOsphere SUPrA Affinity Columns, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system
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12009323
Pkg of 1, 5 ml, prepacked UNOsphere SUPrA Affinity Column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

Cartridges

Cartridges

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7324200
1 ml, prepacked, UNOsphere SUPrA affinity media cartridge
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7324201
Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge
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7324202
5 ml, prepacked, UNOsphere SUPrA affinity media cartridge
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7324408
UNOsphere SUPrA™ affinity cartridge, UNOsphere™ Q cartridge, CHT™ Type 1, 40 µm cartridge, 1 x 5 ml each

Documents

Documents

Number Description Options
10014430
Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media, Rev C
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6867
UNOsphere™ Chromatography Resins Publications List, Ver A
6861
Monoclonal Antibody Purification: Capture Purification Resins Brochure, Ver A
6886
Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A