UNOsphere SUPrA™ Resin

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UNOsphere SUPrA Resin

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732-4120 732-4122 732-4124
UNOsphere™ Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 732-4100 732-4102 732-4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732-4110 732-4112 732-4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732-4140 732-4142 732-4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732-4130 732-4132 732-4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732-4632 732-4634
Antibody Purification
Protein A UNOsphere SUPrA™ Affinity Recombinant protein A 30 mg/ml human IgG 732-4200, 1 x 1 ml 732-4201, 5 x 1 ml 732-4202, 1 x 5 ml
Protein A Affi-Prep® Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600 732-4602
CHT™ Type I CHT Ceramic
Hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 732-4322 732-4324
CHT Type II CHT Ceramic
Hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 732-4332 732-4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
780-0811 780-0812
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732-4610 732-4612 732-4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732-4642 732-4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732-4502 732-4504 732-5304 732-5312 732-5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml
IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml
IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)
Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Monoclonal Antibody Purification Workflow
Monoclonal Antibody Purification Workflow

Workflow for mAb purification with UNOsphere SUPra affinity, UNOsphere Q and CHT type I media.

PDF Bulletin 5728 To read more click here

Flow Performance of UNOsphere SUPrA Media

Narrow elution profile of antibody on UNOsphere SUPrA media.

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UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material from animal origin.

The UNOsphere™ base bead is a macroporous polymeric bead that is designed for robust and scaleable process applications. The outstanding flow-pressure performance of UNOsphere chromatography resin allows their use in large-scale process applications.

UNOsphere SUPrA affinity chromatography supports come with full regulatory support and are backed by the support of Bio-Rad’s global application and development team.

If you are interested in ordering large-volume quantities, please contact your local Bio-Rad representative to discuss your requirements.

Composition Highly cross-linked polymer
Particle size range 53–61 µm
Ligand Recombinant protein A
Coupling chemistry Epoxy
Working pH range 3–11
Dynamic binding capacity* 30 ±3 mg/ml at 150 cm/hr
25 ±2 mg/ml at 300 cm/hr
20 ±2 mg/ml at 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical stability 10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-in-place (CIP) solutions 6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range 100–600 cm/hr
Temperature stability 4–40°C
Delivery conditions 50% slurry in 20% ethanol
Storage conditions 4–8°C
* 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.  
 
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UNOsphere SUPrA Affinity Chromatography Media

1560218
25 ml, protein A affinity chromatography media

0 1560218 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0218_0921120655973.xml 1560218 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldop 2017-11-14 07:08:03 true true internet/product_SKU jthvldop templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0218_0921120655973.xml LSR 2019-05-04 20:56:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998738314 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0218_0921120655973.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0218_0921120655973.xml 1560218-unosphere-supra-affinity-chromatography-media 1560218 UNOsphere SUPrA Affinity Chromatography Media UNOsphere SUPrA Affinity Chromatography Media 25 ml, protein A affinity chromatography media 25 ml, protein A affinity chromatography media 25 ml, protein A affinity chromatography media /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0218_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0218_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0218_feature.jpg RepEx 156-0218 25 ml, protein A affinity chromatography media 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 1560218, unosphere, supra, affinity, chromatography, media, medium, recombinant, protein, process-scale, purification, monoclonal, antibodies, 25, ml false null false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560218&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560218&CartQuote=QT&quantity= 1262 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560218&AddToDefaultWishList=true&quantity= 23151800 null 1560218
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UNOsphere SUPrA Affinity Chromatography Media

1560219
100 ml, protein A affinity chromatography media

1 1560219 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0219_0921120655993.xml 1560219 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldow 2017-11-14 07:08:03 true true internet/product_SKU jthvldow templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0219_0921120655993.xml LSR 2019-05-04 20:56:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998738356 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0219_0921120655993.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0219_0921120655993.xml 1560219-unosphere-supra-affinity-chromatography-media 1560219 UNOsphere SUPrA Affinity Chromatography Media UNOsphere SUPrA Affinity Chromatography Media 100 ml, protein A affinity chromatography media 100 ml, protein A affinity chromatography media 100 ml, protein A affinity chromatography media /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0219_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0219_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0219_feature.jpg RepEx 156-0219 100 ml, protein A affinity chromatography media 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 1560219, unosphere, supra, affinity, chromatography, media, medium, recombinant, protein, process-scale, purification, monoclonal, antibodies, 50, ml false null false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560219&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560219&CartQuote=QT&quantity= 1262 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560219&AddToDefaultWishList=true&quantity= 23151800 null 1560219
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UNOsphere SUPrA Affinity Chromatography Media

156-0220
500 ml, protein A affinity chromatography media

2 1560220 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0220_0921120656024.xml 1560220 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldm0 2017-11-14 07:08:03 true true internet/product_SKU jthvldm0 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0220_0921120656024.xml LSR 2019-05-04 20:55:54 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998738398 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:54 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0220_0921120656024.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0220_0921120656024.xml 1560220-unosphere-supra-affinity-chromatography-media 1560220 UNOsphere SUPrA Affinity Chromatography Media UNOsphere SUPrA Affinity Chromatography Media 500 ml, protein A affinity chromatography media 500 ml, protein A affinity chromatography media 500 ml, protein A affinity chromatography media /webroot/web/images/lsr/products/chromatography/sku_thumb/global/156-0220_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/156-0220_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/156-0220_feature.jpg RepEx 156-0220 500 ml, protein A affinity chromatography media 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 1560220, unosphere, supra, affinity, chromatography, media, medium, recombinant, protein, process-scale, purification, monoclonal, antibodies, 100, ml false null false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560220&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560220&CartQuote=QT&quantity= 1262 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560220&AddToDefaultWishList=true&quantity= 12000000 null 156-0220
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(Discontinued) UNOsphere SUPrA Affinity Chromatography Media

156-0221
5 L, protein A affinity chromatography media

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) UNOsphere SUPrA Affinity Chromatography Media

156-0222
10 L, protein A affinity chromatography media

This product is no longer available. Please contact technical support for more information on replacement product.
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 1 ml

7324200
1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

7324200 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4200_0921120646858.xml 5 7324200 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldi1 2017-11-14 07:07:56 true true internet/product_SKU jthvldi1 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4200_0921120646858.xml LSR 2019-05-04 20:55:43 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997272532 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:43 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4200_0921120646858.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4200_0921120646858.xml 7324200-bio-scale-mini-unosphere-supra-affinity-chromatography-cartridges-1-x-1-ml 7324200 Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 1 ml Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 1 ml 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4200_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4200_view.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4200_view.jpg RepEx 732-4200 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 false $176.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324200&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324200&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324200&AddToDefaultWishList=true&quantity= 41115700 176.00 7324200
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 5 x 1 ml

7324201
Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

7324201 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4201_0921120646815.xml 6 7324201 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldkn 2017-11-14 07:07:56 true true internet/product_SKU jthvldkn templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4201_0921120646815.xml LSR 2019-05-04 20:55:51 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997272574 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:51 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4201_0921120646815.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4201_0921120646815.xml 7324201-bio-scale-mini-unosphere-supra-affinity-chromatography-cartridges-5-x-1-ml 7324201 Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 5 x 1 ml Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 5 x 1 ml Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4201_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4201_view.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4201_view.jpg RepEx 732-4201 Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 false $750.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324201&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324201&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324201&AddToDefaultWishList=true&quantity= 12000000 750.00 7324201
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 5 ml

7324202
5 ml, prepacked, UNOsphere SUPrA affinity media cartridge

7324202 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4202_0921120646837.xml 7 7324202 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldiw 2017-11-14 07:07:56 true true internet/product_SKU jthvldiw templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4202_0921120646837.xml LSR 2019-05-04 20:55:45 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555997272616 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:45 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4202_0921120646837.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/732-4202_0921120646837.xml 7324202-bio-scale-mini-unosphere-supra-affinity-chromatography-cartridges-1-x-5-ml 7324202 Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 5 ml Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 5 ml 5 ml, prepacked, UNOsphere SUPrA affinity media cartridge 5 ml, prepacked, UNOsphere SUPrA affinity media cartridge 5 ml, prepacked, UNOsphere SUPrA affinity media cartridge /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4202_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4202_view.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4202_view.jpg RepEx 732-4202 5 ml, prepacked, UNOsphere SUPrA affinity media cartridge 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Columns N 0 false $632.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324202&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324202&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324202&AddToDefaultWishList=true&quantity= 23151800 632.00 7324202
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Bio-Scale™ Mini mAb Purification Cartridge Kit

7324408
UNOsphere SUPrA™ affinity cartridge, UNOsphere™ Q cartridge, CHT™ Type 1, 40 µm cartridge, 1 x 5 ml each

7324408 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/732-4408_0921120655931.xml 8 7324408 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jao0tf60 2017-11-14 07:08:03 true true internet/product_SKU jao0tf60 templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/732-4408_0921120655931.xml LSR 2019-05-04 20:55:52 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998740001 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:55:52 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/732-4408_0921120655931.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/732-4408_0921120655931.xml 7324408-bio-scale-mini-mab-purification-cartridge-kit 7324408 Bio-Scale Mini MAb Purification Cartridge Kit, 5ml each Bio-Scale™ Mini mAb Purification Cartridge Kit UNOsphere SUPrA™ affinity cartridge, UNOsphere™ Q cartridge, CHT™ Type 1, 40 µm cartridge, 1 x 5 ml each Kit contains one each, 5 ml, prepacked, UNOsphere SUPrA affinity media cartridge, UNOsphere Q media cartridge, and CHT ceramic hydroxyapatite Type I cartridge UNOsphere SUPrA™ affinity cartridge, UNOsphere™ Q cartridge, CHT™ Type 1, 40 µm cartridge, 1 x 5 ml each /webroot/web/images/lsr/products/chromatography/sku_thumb/global/732-4408_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/732-4408_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/732-4408_feature.jpg RepEx 732-4408 Kit contains one each, 5 ml, prepacked, UNOsphere SUPrA affinity media cartridge, UNOsphere Q media cartridge, and CHT ceramic hydroxyapatite Type I cartridge 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 ,VN / false $493.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324408&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324408&CartQuote=QT&quantity= 7651 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7324408&AddToDefaultWishList=true&quantity= 41115700 493.00 7324408
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Number Description Options
10014430 Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media, Rev C Click to download
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download
6786 Monoclonal Antibody Purification Resin Selection Card, Ver A Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6867 UNOsphere™ Chromatography Resins Publications List, Ver A Click to download
6861 Monoclonal Antibody Purification: Capture Purification Resins Brochure, Ver A Click to download
6886 Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A Click to download

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