Reliance One-Step Multiplex RT-qPCR Supermix

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Reliance One-Step Multiplex RT-qPCR  Supermix | Bio-Rad.com

Overview

Exceptional Performance of 5-Target Multiplex

Five targets across seven orders of magnitude (1 pg to 1 μg RNA input) were amplified using Reliance One-Step Multiplex Supermix on a CFX96 Touch Real-Time System. The data demonstrated exceptional performance with superior efficiency and sensitivity over a wide dynamic range down to 1 pg of RNA input.

Exceptional 5-Plex Performance

Unparalleled Stability of Assembled Reactions

Five targets across seven orders of magnitude (1 pg to 1 μg RNA input) were amplified using various reagents on a CFX96 Touch Real-Time System immediately after the reaction set up and then again after 24 hours incubation at room temperature protected from light. Reliance One-Step Multiplex Supermix maintained less than 1 ΔCq between two runs () indicating uncompromised performance even after 24 hour incubation of assembled reactions while other suppliers’ reagents showed significantly declined performance with over 1 ΔCq () or no amplification ().

Exceptional Room Temperature Stability

Co-detection of RNA and DNA Targets in 5-Plex Reaction

An experiment was designed to test samples containing three viral nucleic acids, two in their RNA form — Zika (ZIKV) and Chikungunya (CHIKV) — and the DNA version of Dengue Virus (DENV), as well as an internal positive control RNA and human genomic DNA control (gDNA). The viral targets were tested at five different dilutions, while the controls were kept constant. The number of copies for the viral RNA targets was determined by Droplet Digital RT-PCR and was diluted to a single copy or less per reaction in their lowest dilution series (Table 1). Two identical plates each containing 10 technical replicates of each sample were prepared; one plate was analyzed immediately (time = 0 hr) while the second plate was stored at room temperature (25 °C) and analyzed the next day (time = 24 hr). Reliance One-Step Multiplex Supermix efficiently detected both RNA and DNA targets with outstanding efficiency and single copy detection sensitivity at both time points (Fig 1.)

Table 1. Targets and templates used for a 5-plex reaction.


Target Reporter Dye Template Amount RNA Copies
ZIKV FAM Viral RNA 5 dilution series 1.17 to 11,668
CHIKV HEX Viral RNA 5 dilution series 0.15 to 1,498
DENV Texas Red Synthetic DNA 5 dilution series N/A
IPC Cy5 Synthetic DNA Constant N/A
RNaseP Cy5.5 Human gDNA Constant N/A

Co-detection of DNA and RNA Targets in 5-plex Reaction
Figure 1. Co-detection of various nucleic acid targets in 5-plex reaction.

Exclusive Sso7d Fusion Polymerase Technology to Empower Your PCR

Sso7d, a 7 kD protein obtained from Sulfolobus solfataricus, binds to dsDNA without any sequence specificity.

Sso7d is covalently linked to a DNA polymerase and is able to stabilize the polymerase-template complex without compromising the structural integrity, thermal stability, or catalytic activity of the enzyme.

The unique structure of this Sso7d fusion polymerase provides several significant advantages over ordinary DNA polymerase:

  • Increased processivity
  • Effective amplification in GC-rich regions or strong secondary structure
  • Efficient amplification of longer products
  • Superior tolerance to PCR inhibitors
Sso7D Technology
Use On Any qPCR System

Passive reference dye signal in low- and high-ROX instruments and using Bio-Rad’s universal reagents.

A. Optimal excitation of the ROX dye at ~575 nm in low-ROX instruments results in a strong ROX signal, while poor excitation at ~488 nm in high-ROX instruments leads to a weak ROX signal, creating a need for additional ROX when using high-ROX instruments. B. A blend of dyes in Bio-Rad’s universal reagents allows excitation of the ROX dye in low-ROX instruments and the long Stoke-shift dye in high-ROX instruments, negating the need for additional ROX in the latter case. Excitation (—); emission (– – –).

Universal Reference Dye Figure A

Universal Reference Dye Figure B

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CFX Loyalty Program

Earn a free one-year service contract on your CFX System with purchase of Reagents.

CFX Loyalty Program

Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.

Features and Benefits

  • Sensitive, reproducible multiplexing of up to 5 targets — get the most out of your samples while reducing costs
  • 24-hour assembled reaction stability at room temperature — enables the use in an automated workflow to maximize throughput
  • Convenient one-tube supermix — for minimizing reaction setup time and handling errors
  • 4x concentrated formulation — increase detection sensitivity by maximizing input volume
  • Tolerant to common PCR inhibitors — for use with lysates or samples of varying quality

Exceptional Performance of 5-target Multiplex

Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 µg of RNA input with maximum sensitivity and efficiency.

Unparalleled Room Temperature Stability for Up To 24 Hours

Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.



Efficient Co-detection of RNA and DNA Targets

Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.

View Application Note (PDF 143KB)

Streamline Your Workflow

4x Reliance One-Step Multiplex Supermix is Formulated with:

  • Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**
  • 4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components
  • Reaction stabilizers
  • Patented*** universal reference dye

Applications and Uses

  • Gene expression analysis
  • Viral/pathogen detection (RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)
  • Diagnostic research
  • High-throughput screening (small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)

Instrument Compatibility

Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0

Choose the Right One-Step RT qPCR Product for Your Needs

  Reliance One-Step Multiplex Supermix iTaq Universal One-Step Probe Kit
Key Features
  • Sensitive, efficient amplification of up to 5 targets
  • Room temperature stable up to 24 hours
  • Reliable amplification of up to 2 targets
  • Compatible with a fast protocol
Ideal for
  • Viral/pathogen detection in high-multiplex reaction
  • High throughput screening
  • Viral/pathogen detection
  • Singleplex and duplex reactions

Related Products

Supporting Documents

  • Amplification Reagents and Plastics Brochure
  • SingleShot Cell Lysis Kit Instruction Manual
  • One-Step Multiplex RT-qPCR Using PrimePCR Probe Assays Protocol
  • Sensitive Codetection of Viral-Pathogen RNA and DNA Using One-Step Multiplex RT-qPCR Application Note

Online Resources

What is Real-Time PCR (qPCR)?

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* Patent pending
** U.S. patents 6,627,424; 7,541,170; and 7,560,260.
*** US patent 9,493,824

Concentration  4x concentrated
Guaranteed shelf life at –20° 12 months
Shelf life at 4°C (after thawing) 1 week
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Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 µl rxns, 1 ml

12010176
1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns

12010176 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml 12010176 04/08/19 08:21 PM en_US true internet/product_SKU 2019-08-13 21:59:53 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml /default/main/bio-rad/WORKAREA/default jthvlism 2019-04-08 22:09:10 true jthvlism templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml LSR N /default/main/bio-rad en 04/08/36 10:26 AM 1565048458003 2019-08-13 21:59:53 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml 12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml 1 12010176 Reliance One-Step Multiplex Supermix Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 µl rxns, 1 ml 1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns 1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/12010176_th.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/12010176.jpg preview Anton Ross Reliance One-Step Multiplex Supermix 1 ml | Bio-Rad.com Use Bio-Rad's 1 ml Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. 12010176, reliance, supermix, one-step multiplex supermix, pcr, amplification 04/08/19 08:21 PM 04/08/36 10:26 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <div class="corner-box corner-box-promo"><a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class=""><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/cfx-loyalty-program-icon.jpg" border="0" alt="CFX Loyalty Program" /></a> <p>Earn a free one-year service contract on your CFX System with purchase of Reagents.</p> <a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window"><strong>CFX Loyalty Program</strong></a></div> <p class="wraptxt">Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.</p> <img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/reliance-1-step-th.png" /> <div class="pdp-header"> <h2 class="overview_header">Features and Benefits</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">Sensitive, reproducible multiplexing of up to 5 targets</a><span>&nbsp;</span>&mdash; get the most out of your samples while reducing costs</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">24-hour assembled reaction stability</a><span>&nbsp;</span>at room temperature &mdash; enables the use in an automated workflow to maximize throughput</li> <li>Convenient one-tube supermix &mdash; for minimizing reaction setup time and handling errors</li> <li>4x concentrated formulation &mdash; increase detection sensitivity by maximizing input volume</li> <li>Tolerant to common PCR inhibitors<span>&nbsp;</span>&mdash; for use with lysates or samples of varying quality</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Exceptional Performance of 5-target Multiplex</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/exceptional-5plex-performance-th.jpg" alt="Exceptional 5-plex Performance" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">View 5-target multiplex data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 &micro;g of RNA input with maximum sensitivity and efficiency.</p> </div> <div class="pdp-header"> <h2 class="overview_header">Unparalleled Room Temperature Stability for Up To 24 Hours</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/exceptional-room-temp-stability-icon.jpg" alt="Exceptional Room Temperature Stability" width="207" height="auto" style="padding-left: 100px;" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">View assembled reaction stability data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.</p> </div> <br /> <br /> <div class="pdp-header"> <h2 class="overview_header">Efficient Co-detection of RNA and DNA Targets</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/codetection-of-dna-rna-th.jpg" alt="Co-detection of DNA and RNA" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')">View co-detection of RNA and DNA data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.</p> <small><a class="documentation-pdf-icon no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_7196.pdf">View Application Note</a> (PDF 143KB)</small></div> <div class="pdp-header"> <h2 class="overview_header">Streamline Your Workflow</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">SingleShot Cell Lysis Kit</a>, for the generation of lysates for use in qPCR or western blots</li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Probe Assays</a> for multiplexing gene expression targets with minimal cross-reactivity and maximum transcript coverage</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">CFX Automation System II</a> for walk-away high-throughput qPCR operation</li> </ul> <div class="pdp-header"> <h2 class="overview_header">4x Reliance One-Step Multiplex Supermix is Formulated with:</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**</li> <li>4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components</li> <li>Reaction stabilizers</li> <li>Patented*** universal reference dye</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Applications and Uses</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Gene expression analysis</li> <li>Viral/pathogen detection&nbsp;(RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)</li> <li>Diagnostic research</li> <li>High-throughput screening&nbsp;(small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Packaging Options</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Kit Size</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">1 ml (1 x 1 ml vial), 200 x 20 &mu;l reactions</td> <td valign="middle">12010176</td> </tr> <tr> <td valign="middle">5 ml (5 x 1 ml vial), 1,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010220-reliance-one-step-multiplex-supermix-1000-x-20-ul-rxns-5-ml?ID=12010220">12010220</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">10 ml (2 x 5 ml vial), 2,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010221-reliance-one-step-multiplex-supermix-2000-x-20-ul-rxns-10-ml?ID=12010221">12010221</a></td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Instrument Compatibility</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Choose the Right One-Step RT qPCR Product for Your Needs</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top">&nbsp;</td> <td valign="top"><strong>Reliance One-Step Multiplex Supermix</strong></td> <td valign="top"><strong>iTaq Universal One-Step Probe Kit</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top"><strong>Key Features</strong></td> <td valign="top"> <ul> <li>Sensitive, efficient amplification of up to 5 targets</li> <li>Room temperature stable up to 24 hours</li> </ul> </td> <td valign="top"> <ul> <li>Reliable amplification of up to 2 targets</li> <li>Compatible with a fast protocol</li> </ul> </td> </tr> <tr> <td valign="top"><strong>Ideal for</strong></td> <td valign="top"> <ul> <li>Viral/pathogen detection in high-multiplex reaction</li> <li>High throughput screening</li> </ul> </td> <td valign="top"> <ul> <li>Viral/pathogen detection</li> <li>Singleplex and duplex reactions</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Related Products</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">Cell Lysis Kits</a></li> <li><a href="../category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Predesigned Probe qPCR Assays for Gene Expression</a> (Available in the U.S., Canada, E.U., China, Australia, New Zealand, Singapore, Taiwan, Hong Kong, Israel, South Africa, Thailand, Macau, Korea, and Japan.)</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">Real-Time PCR Automation System</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Supporting Documents</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Brochure</a></li> <li>SingleShot Cell Lysis Kit <a href="/webroot/web/pdf/lsr/literature/10000112808.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Instruction Manual</a></li> <li>One-Step Multiplex RT-qPCR Using PrimePCR Probe Assays <a href="/webroot/web/pdf/lsr/literature/Bulletin_7197.pdf" class="no-target-style">Protocol</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Online Resources</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p><a href="/en-us/applications-technologies/what-real-time-pcr-qpcr?ID=LUSO4W8UU" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window">What is Real-Time PCR (qPCR)?</a></p> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">More Information</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/en-us/applications-technologies/qpcr-assay-design-optimization?ID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into analysis of <a href="/en-us/applications-technologies/real-time-pcr-data-analysis?ID=LUSOA9LPT">qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">troubleshooting reactions</a>.</p> <p>* Patent pending<br />** U.S. patents 6,627,424; 7,541,170; and 7,560,260.<br /> *** US patent 9,493,824</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 12010176.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/12010176.html Concentration  1484 4x concentrated 1 0 Guaranteed shelf life at –20° 3104 12 months 1 0 Shelf life at 4°C (after thawing) 3105 1 week 1 0 /en-us/life-science-research/promotions/save-30-all-sizes-reliance-one-step-supermix false $409.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&AddToDefaultWishList=true&quantity= 41106300 409.00 12010176
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Reliance One-Step Multiplex Supermix, 1,000 x 20 µl rxns, 5 ml

12010220
5 ml (5 x 1 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 1,000 x 20 μl rxns

12010220 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010220_1554856328.xml 12010220 04/10/19 12:24 AM en_US true internet/product_SKU 2019-08-13 21:59:53 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010220_1554856328.xml /default/main/bio-rad/WORKAREA/default jthvlj8k 2019-04-10 00:32:08 true jthvlj8k templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010220_1554856328.xml LSR N /default/main/bio-rad en 04/09/36 02:27 PM 1565061941231 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010220_1554856328.xml 2019-08-13 21:59:53 12010220-reliance-one-step-multiplex-supermix-1000-x-20-ul-rxns-5-ml 1 12010220 Reliance One-Step Multiplex Supermix Reliance One-Step Multiplex Supermix, 1,000 x 20 µl rxns, 5 ml 5 ml (5 x 1 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 1,000 x 20 μl rxns 5 ml (5 x 1 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 1,000 x 20 μl rxns /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/12010220_th.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/12010220.jpg preview Anton Ross Reliance One-Step Multiplex Supermix 5 ml | Bio-Rad.com Reliance One-Step Multiplex Supermix (5 ml) is an ideal solution for multiplexed RT-qPCR, including pathogen or viral detection and high throughput screening applications. 12010220, supermix, reliance one-step multiplex supermix, reliance one-step multiplex, reliance one-step, reliance one step, super mix 04/10/19 12:24 AM 04/09/36 02:27 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <div class="corner-box corner-box-promo"><a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class=""><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/cfx-loyalty-program-icon.jpg" border="0" alt="CFX Loyalty Program" /></a> <p>Earn a free one-year service contract on your CFX System with purchase of Reagents.</p> <a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window"><strong>CFX Loyalty Program</strong></a></div> <p class="wraptxt">Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.</p> <img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/reliance-1-step-th.png" /> <div class="pdp-header"> <h2 class="overview_header">Features and Benefits</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">Sensitive, reproducible multiplexing of up to 5 targets</a><span>&nbsp;</span>&mdash; get the most out of your samples while reducing costs</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">24-hour assembled reaction stability</a><span>&nbsp;</span>at room temperature &mdash; enables the use in an automated workflow to maximize throughput</li> <li>Convenient one-tube supermix &mdash; for minimizing reaction setup time and handling errors</li> <li>4x concentrated formulation &mdash; increase detection sensitivity by maximizing input volume</li> <li>Tolerant to common PCR inhibitors<span>&nbsp;</span>&mdash; for use with lysates or samples of varying quality</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Exceptional Performance of 5-target Multiplex</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/exceptional-5plex-performance-th.jpg" alt="Exceptional 5-plex Performance" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">View 5-target multiplex data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 &micro;g of RNA input with maximum sensitivity and efficiency.</p> </div> <div class="pdp-header"> <h2 class="overview_header">Unparalleled Room Temperature Stability for Up To 24 Hours</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/exceptional-room-temp-stability-icon.jpg" alt="Exceptional Room Temperature Stability" width="207" height="auto" style="padding-left: 100px;" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">View assembled reaction stability data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.</p> </div> <br /> <br /> <div class="pdp-header"> <h2 class="overview_header">Efficient Co-detection of RNA and DNA Targets</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/codetection-of-dna-rna-th.jpg" alt="Co-detection of DNA and RNA" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')">View co-detection of RNA and DNA data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.</p> <small><a class="documentation-pdf-icon no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_7196.pdf">View Application Note</a> (PDF 143KB)</small></div> <div class="pdp-header"> <h2 class="overview_header">Streamline Your Workflow</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">SingleShot Cell Lysis Kit</a>, for the generation of lysates for use in qPCR or western blots</li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Probe Assays</a> for multiplexing gene expression targets with minimal cross-reactivity and maximum transcript coverage</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">CFX Automation System II</a> for walk-away high-throughput qPCR operation</li> </ul> <div class="pdp-header"> <h2 class="overview_header">4x Reliance One-Step Multiplex Supermix is Formulated with:</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**</li> <li>4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components</li> <li>Reaction stabilizers</li> <li>Patented*** universal reference dye</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Applications and Uses</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Gene expression analysis</li> <li>Viral/pathogen detection&nbsp;(RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)</li> <li>Diagnostic research</li> <li>High-throughput screening&nbsp;(small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Packaging Options</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Kit Size</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">1 ml (1 x 1 ml vial), 200 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml?ID=12010176">12010176</a></td> </tr> <tr> <td valign="middle">5 ml (5 x 1 ml vial), 1,000 x 20 &mu;l reactions</td> <td valign="middle">12010220</td> </tr> <tr class="pd_colorbackground"> <td valign="middle">10 ml (2 x 5 ml vial), 2,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010221-reliance-one-step-multiplex-supermix-2000-x-20-ul-rxns-10-ml?ID=12010221">12010221</a></td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Instrument Compatibility</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Choose the Right One-Step RT qPCR Product for Your Needs</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top">&nbsp;</td> <td valign="top"><strong>Reliance One-Step Multiplex Supermix</strong></td> <td valign="top"><strong>iTaq Universal One-Step Probe Kit</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top"><strong>Key Features</strong></td> <td valign="top"> <ul> <li>Sensitive, efficient amplification of up to 5 targets</li> <li>Room temperature stable up to 24 hours</li> </ul> </td> <td valign="top"> <ul> <li>Reliable amplification of up to 2 targets</li> <li>Compatible with a fast protocol</li> </ul> </td> </tr> <tr> <td valign="top"><strong>Ideal for</strong></td> <td valign="top"> <ul> <li>Viral/pathogen detection in high-multiplex reaction</li> <li>High throughput screening</li> </ul> </td> <td valign="top"> <ul> <li>Viral/pathogen detection</li> <li>Singleplex and duplex reactions</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Related Products</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">Cell Lysis Kits</a></li> <li><a href="../category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Predesigned Probe qPCR Assays for Gene Expression</a> (Available in the U.S., Canada, E.U., China, Australia, New Zealand, Singapore, Taiwan, Hong Kong, Israel, South Africa, Thailand, Macau, Korea, and Japan.)</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">Real-Time PCR Automation System</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Supporting Documents</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Brochure</a></li> <li>SingleShot Cell Lysis Kit <a href="/webroot/web/pdf/lsr/literature/10000112808.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Instruction Manual</a></li> <li>One-Step Multiplex RT-qPCR Using PrimePCR Probe Assays <a href="/webroot/web/pdf/lsr/literature/Bulletin_7197.pdf" class="no-target-style">Protocol</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Online Resources</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p><a href="/en-us/applications-technologies/what-real-time-pcr-qpcr?ID=LUSO4W8UU" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window">What is Real-Time PCR (qPCR)?</a></p> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">More Information</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/en-us/applications-technologies/qpcr-assay-design-optimization?ID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into analysis of <a href="/en-us/applications-technologies/real-time-pcr-data-analysis?ID=LUSOA9LPT">qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">troubleshooting reactions</a>.</p> <p>* Patent pending<br />** U.S. patents 6,627,424; 7,541,170; and 7,560,260.<br /> *** US patent 9,493,824</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 12010220.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/12010220.html Concentration  1484 4x concentrated 1 0 Guaranteed shelf life at –20° 3104 12 months 1 0 Shelf life at 4°C (after thawing) 3105 1 week 1 0 /en-us/life-science-research/promotions/save-30-all-sizes-reliance-one-step-supermix false $1,881.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010220&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010220&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010220&AddToDefaultWishList=true&quantity= 41106300 1881.00 12010220
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Reliance One-Step Multiplex Supermix, 2,000 x 20 µl rxns, 10 ml

12010221
10 ml (2 x 5 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 2,000 x 20 μl rxns

12010221 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010221_1554855875.xml 12010221 04/10/19 12:19 AM en_US true internet/product_SKU 2019-08-13 21:59:53 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010221_1554855875.xml /default/main/bio-rad/WORKAREA/default jthvlj8j 2019-04-10 00:24:35 true jthvlj8j templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010221_1554855875.xml LSR N /default/main/bio-rad en 04/09/29 02:21 PM 1565054798883 2019-08-13 21:59:53 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010221_1554855875.xml 12010221-reliance-one-step-multiplex-supermix-2000-x-20-ul-rxns-10-ml 1 12010221 Reliance One-Step Multiplex Supermix Reliance One-Step Multiplex Supermix, 2,000 x 20 µl rxns, 10 ml 10 ml (2 x 5 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 2,000 x 20 μl rxns 10 ml (2 x 5 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 2,000 x 20 μl rxns /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/12010221_th.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/12010221.jpg preview Anton Ross Reliance One-Step Multiplex Supermix 10 ml | Bio-Rad Bio-Rad's 10 ml Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, including pathogen or viral detection & high throughput screening applications. 12010221, 10 ml supermix, 10 ml reliance one-step multiplex supermix, 10ml reliance one-step supermix, 10ml reliance one-step, 10 ml reliance 04/10/19 12:19 AM 04/09/29 02:21 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <div class="corner-box corner-box-promo"><a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class=""><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/cfx-loyalty-program-icon.jpg" border="0" alt="CFX Loyalty Program" /></a> <p>Earn a free one-year service contract on your CFX System with purchase of Reagents.</p> <a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window"><strong>CFX Loyalty Program</strong></a></div> <p class="wraptxt">Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.</p> <img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/reliance-1-step-th.png" /> <div class="pdp-header"> <h2 class="overview_header">Features and Benefits</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">Sensitive, reproducible multiplexing of up to 5 targets</a><span>&nbsp;</span>&mdash; get the most out of your samples while reducing costs</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">24-hour assembled reaction stability</a><span>&nbsp;</span>at room temperature &mdash; enables the use in an automated workflow to maximize throughput</li> <li>Convenient one-tube supermix &mdash; for minimizing reaction setup time and handling errors</li> <li>4x concentrated formulation &mdash; increase detection sensitivity by maximizing input volume</li> <li>Tolerant to common PCR inhibitors<span>&nbsp;</span>&mdash; for use with lysates or samples of varying quality</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Exceptional Performance of 5-target Multiplex</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/exceptional-5plex-performance-th.jpg" alt="Exceptional 5-plex Performance" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">View 5-target multiplex data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 &micro;g of RNA input with maximum sensitivity and efficiency.</p> </div> <div class="pdp-header"> <h2 class="overview_header">Unparalleled Room Temperature Stability for Up To 24 Hours</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/exceptional-room-temp-stability-icon.jpg" alt="Exceptional Room Temperature Stability" width="207" height="auto" style="padding-left: 100px;" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">View assembled reaction stability data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.</p> </div> <br /> <br /> <div class="pdp-header"> <h2 class="overview_header">Efficient Co-detection of RNA and DNA Targets</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/codetection-of-dna-rna-th.jpg" alt="Co-detection of DNA and RNA" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')">View co-detection of RNA and DNA data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.</p> <small><a class="documentation-pdf-icon no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_7196.pdf">View Application Note</a> (PDF 143KB)</small></div> <div class="pdp-header"> <h2 class="overview_header">Streamline Your Workflow</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">SingleShot Cell Lysis Kit</a>, for the generation of lysates for use in qPCR or western blots</li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Probe Assays</a> for multiplexing gene expression targets with minimal cross-reactivity and maximum transcript coverage</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">CFX Automation System II</a> for walk-away high-throughput qPCR operation</li> </ul> <div class="pdp-header"> <h2 class="overview_header">4x Reliance One-Step Multiplex Supermix is Formulated with:</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**</li> <li>4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components</li> <li>Reaction stabilizers</li> <li>Patented*** universal reference dye</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Applications and Uses</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Gene expression analysis</li> <li>Viral/pathogen detection&nbsp;(RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)</li> <li>Diagnostic research</li> <li>High-throughput screening&nbsp;(small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Packaging Options</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Kit Size</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">1 ml (1 x 1 ml vial), 200 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml?ID=12010176">12010176</a></td> </tr> <tr> <td valign="middle">5 ml (5 x 1 ml vial), 1,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010220-reliance-one-step-multiplex-supermix-1000-x-20-ul-rxns-5-ml?ID=12010220">12010220</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">10 ml (2 x 5 ml vial), 2,000 x 20 &mu;l reactions</td> <td valign="middle">12010221</td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Instrument Compatibility</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Choose the Right One-Step RT qPCR Product for Your Needs</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top">&nbsp;</td> <td valign="top"><strong>Reliance One-Step Multiplex Supermix</strong></td> <td valign="top"><strong>iTaq Universal One-Step Probe Kit</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top"><strong>Key Features</strong></td> <td valign="top"> <ul> <li>Sensitive, efficient amplification of up to 5 targets</li> <li>Room temperature stable up to 24 hours</li> </ul> </td> <td valign="top"> <ul> <li>Reliable amplification of up to 2 targets</li> <li>Compatible with a fast protocol</li> </ul> </td> </tr> <tr> <td valign="top"><strong>Ideal for</strong></td> <td valign="top"> <ul> <li>Viral/pathogen detection in high-multiplex reaction</li> <li>High throughput screening</li> </ul> </td> <td valign="top"> <ul> <li>Viral/pathogen detection</li> <li>Singleplex and duplex reactions</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Related Products</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">Cell Lysis Kits</a></li> <li><a href="../category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Predesigned Probe qPCR Assays for Gene Expression</a> (Available in the U.S., Canada, E.U., China, Australia, New Zealand, Singapore, Taiwan, Hong Kong, Israel, South Africa, Thailand, Macau, Korea, and Japan.)</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">Real-Time PCR Automation System</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Supporting Documents</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Brochure</a></li> <li>SingleShot Cell Lysis Kit <a href="/webroot/web/pdf/lsr/literature/10000112808.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Instruction Manual</a></li> <li>One-Step Multiplex RT-qPCR Using PrimePCR Probe Assays <a href="/webroot/web/pdf/lsr/literature/Bulletin_7197.pdf" class="no-target-style">Protocol</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Online Resources</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p><a href="/en-us/applications-technologies/what-real-time-pcr-qpcr?ID=LUSO4W8UU" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window">What is Real-Time PCR (qPCR)?</a></p> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">More Information</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/en-us/applications-technologies/qpcr-assay-design-optimization?ID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into analysis of <a href="/en-us/applications-technologies/real-time-pcr-data-analysis?ID=LUSOA9LPT">qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">troubleshooting reactions</a>.</p> <p>* Patent pending<br />** U.S. patents 6,627,424; 7,541,170; and 7,560,260.<br /> *** US patent 9,493,824</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 12010221.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/12010221.html Concentration  1484 4x concentrated 1 0 Guaranteed shelf life at –20° 3104 12 months 1 0 Shelf life at 4°C (after thawing) 3105 1 week 1 0 /en-us/life-science-research/promotions/save-30-all-sizes-reliance-one-step-supermix false $3,564.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010221&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010221&CartQuote=QT&quantity= 1468 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010221&AddToDefaultWishList=true&quantity= 41106300 3564.00 12010221
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SingleShot Cell Lysis Kit, 100 x 50 µl rxns

1725080
Pkg of 1, kit includes 5 ml (1 x 5 ml) of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions

1725080 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml 1725080 06/04/14 10:53 AM en_US true internet/product_SKU 2019-06-19 17:28:41 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml /default/main/bio-rad/WORKAREA/default jcrucywt 2017-11-14 07:07:36 true jcrucywt templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml LSR N /default/main/bio-rad en 05/15/29 12:52 AM 1558748949279 2019-06-19 17:28:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml 1725080-singleshot-cell-lysis-kit-100-x-50-ul-rxns 1 1725080 SingleShotCellLysisKit SingleShot Cell Lysis Kit, 100 x 50 µl rxns Pkg of 1, kit includes 5 ml <span class="forcebreaks"> (1 x 5 ml)</span> of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions Pkg of 1, kit includes 5 ml <span class="forcebreaks"> (1 x 5 ml)</span> of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/single-shot-cell-lysis-kit-172-5080-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/single-shot-cell-lysis-kit-172-5080.jpg SingleShot Cell Lysis Kit preview Karen Moss SingleShot Cell Lysis Kit SingleShot Cell Lysis Kit, 100 x 50 µl reactions 1725080, singleshot, cell, lysis, 100x50, reactions, break, down, rna, cdna, one-step, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification, libertine, embassea, catalina42 06/04/14 10:53 AM 05/15/29 12:52 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot Cell Lysis Kit</strong> to rapidly generate cell lysates that are suitable for RT-qPCR analysis without RNA purification.</p> <p>Kit works on 10 to 100,000 cells in 20 minutes while providing the following key benefits:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html');">linear dynamic range</a> of qPCR data across genes</li> <li>Results comparable to using purified RNA, including dynamic range</li> <li>Validated with PrimePCR Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control included to ensure optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot Cell Lysis Kit maximizes cell lysate and cDNA inputs in RT-qPCR reactions:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT reaction</td> <td>45% of total volume</td> </tr> <tr class="pd_colorbackground"> <td>Maximum undiluted cDNA into qPCR reaction</td> <td>20% of total volume</td> </tr> <tr> <td>Maximum cell lysate into one-step RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <h2 class="descHeader">Kit Contents</h2> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td align="left" valign="top"> <p><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer&nbsp;<br /> SingleShot Proteinase K Solution&nbsp;<br /> SingleShot DNase Solution</p> </td> <td align="left" valign="top"> <p><strong>100 x 50 &micro;l reactions</strong> <br /> 5 ml (1 x 5 ml vial)<br /> 100 &micro;l (1 x 1 ml vial)<br /> 100 &micro;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042477.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot Cell Lysis Kit</h2> <p>The SingleShot Cell Lysis RT-qPCR Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG&nbsp;&nbsp;</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> <h2 class="descHeader">RT-qPCR Step Reagents (Sold Separately)</h2> <p>Reverse Transcription</p> <ul> <li>iScript Advanced cDNA Synthesis Kit for RT-qPCR (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5038">172-5038</a>)</li> </ul> <p>Two-Step RT-qPCR Reagents&nbsp;&nbsp;</p> <ul> <li>SsoAdvanced Universal SYBR&nbsp;Green Supermix (<a href="/en-us/sku/172-5271-ssoadvanced-universal-sybr-green-supermix">172-5271</a>)</li> <li>SsoAdvanced Universal Probes Supermix (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5281">172-5281</a>)</li> </ul> <p>One-Step RT-qPCR Reagents</p> <ul> <li>iTaq Universal SYBR&nbsp;Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5151">172-5151</a>)</li> <li>iTaq Universal Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5141">172-5141</a>)</li> <li>Reliance One-Step Multiplex Supermix (<a href="/en-us/sku/12010220-reliance-one-step-multiplex-supermix-1000-x-20-ul-rxns-5-ml?ID=12010220">12010220</a>)</li> </ul> <p><a href="/en-us/prime-pcr-assays/assay/qhsactlp0001001-primepcr-reverse-transcription-control-probe-assay--human">PrimePCR Reverse Transcription Control Assay</a></p> <p>Use of the optional PrimePCR Reverse Transcription Control Assay in a small pilot experiment enables the determination of the optimal cell count and lysate inputs. The control can be added at the beginning to monitor the lysis step and determine the optimal cell count, or during the qPCR step to monitor RT-qPCR inhibition.</p> <h2 class="descHeader">Related Products</h2> <p>The SingleShot Cell Lysis Kits belong to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot Cell Lysis RT-qPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot SYBR<sup>&nbsp;</sup>Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot SYBR&nbsp;Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <p> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#rNaseinhibitor').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#cellLysates').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> </p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-cell lysis-kit-172-5080-desc2.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-cell lysis-kit-172-5080-desc2.html Storage at –20°C 2199 <strong>12 months:</strong><br />Lysis buffer<br /> DNase solution<br /> Proteinase K solution 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer 1 0 1725038 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5038_1403297461.xml 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml false $509.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725080&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725080&CartQuote=QT&quantity= 1450 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725080&AddToDefaultWishList=true&quantity= 24121500 509.00 1725080
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SingleShot Cell Lysis Kit, 500 x 50 µl rxns

1725081
Pkg of 1, kit includes 25 ml (5 x 5 ml) of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions

1725081 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml 1725081 06/04/14 02:06 PM en_US true internet/product_SKU 2019-05-25 01:57:55 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml /default/main/bio-rad/WORKAREA/default jcrucyxx 2017-11-14 07:07:36 true jcrucyxx templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml LSR N /default/main/bio-rad en 05/23/29 08:02 AM 1556988418420 2019-05-25 01:57:55 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml 1725081-singleshot-cell-lysis-kit-500-x-50-ul-rxns 1 1725081 SingleShotCellLysisKit SingleShot Cell Lysis Kit, 500 x 50 µl rxns Pkg of 1, kit includes 25 ml <span class="forcebreaks"> (5 x 5 ml)</span> of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions Pkg of 1, kit includes 25 ml <span class="forcebreaks"> (5 x 5 ml)</span> of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/single-shot-cell-lysis-kit-172-5081-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/single-shot-cell-lysis-kit-172-5081.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/single-shot-cell-lysis-kit-172-5081.jpg preview Karen Moss SingleSho Cell Lysis Kit SingleShot Cell Lysis Kit, 500 x 50 µl reactions 1725081, singleshot, cell, lysis, 500x50, reactions, break, down, rna, cdna, one-step, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 02:06 PM 05/23/29 08:02 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the<strong> SingleShot Cell Lysis Kit</strong> to rapidly generate cell lysates that are suitable for RT-qPCR analysis without RNA purification.</p> <p>Kit works on 10 to 100,000 cells in 20 minutes while providing the following key benefits:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html');">linear dynamic range</a> of qPCR data across genes</li> <li>Results comparable to using purified RNA, including dynamic range</li> <li>Validated with PrimePCR Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control included to ensure optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot Cell Lysis Kit maximizes cell lysate and cDNA inputs in RT-qPCR reactions:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT reaction</td> <td>45% of total volume</td> </tr> <tr class="pd_colorbackground"> <td>Maximum undiluted cDNA into qPCR reaction</td> <td>20% of total volume</td> </tr> <tr> <td>Maximum cell lysate into one-step RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <h2 class="descHeader">Kit Contents</h2> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td align="left" valign="top"> <p><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer&nbsp;<br /> SingleShot Proteinase K Solution&nbsp;<br /> SingleShot DNase Solution</p> </td> <td align="left" valign="top"> <p><strong>500 x 50 &micro;l reactions</strong> <br /> 25 ml (5 x 5 ml vial)<br /> 500 &micro;l (1 x 1 ml vial)<br /> 500 &micro;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042477.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot Cell Lysis Kit</h2> <p>The SingleShot Cell Lysis Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG&nbsp;&nbsp;</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> <h2 class="descHeader">RT-qPCR Step Reagents (Sold Separately)</h2> <p>Reverse Transcription</p> <ul> <li>iScript Advanced cDNA Synthesis Kit for RT-qPCR (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5038">172-5038</a>)</li> </ul> <p>Two-Step RT-qPCR Reagents&nbsp;&nbsp;</p> <ul> <li>SsoAdvanced Universal SYBR&nbsp;Green Supermix (<a href="/en-us/sku/172-5271-ssoadvanced-universal-sybr-green-supermix">172-5271</a>)</li> <li>SsoAdvanced Universal Probes Supermix (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5281">172-5281</a>)</li> </ul> <p>One-Step RT-qPCR Reagents</p> <ul> <li>iTaq Universal SYBR&nbsp;Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5151">172-5151</a>)</li> <li>iTaq Universal Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5141">172-5141)</a></li> <li>Reliance One-Step Multiplex Supermix (<a href="/en-us/sku/12010220-reliance-one-step-multiplex-supermix-1000-x-20-ul-rxns-5-ml?ID=12010220">12010220</a>)</li> </ul> <p><a href="/en-us/prime-pcr-assays/assay/qhsactlp0001001-primepcr-reverse-transcription-control-probe-assay--human">PrimePCR Reverse Transcription Control Assay</a></p> <p>Use of the optional PrimePCR Reverse Transcription Control Assay in a small pilot experiment enables the determination of the optimal cell count and lysate inputs. The control can be added at the beginning to monitor the lysis step and determine the optimal cell count, or during the qPCR step to monitor RT-qPCR inhibition.</p> <h2 class="descHeader">Related Products</h2> <p>The SingleShot Cell Lysis Kits belong to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot Cell Lysis RT-qPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot SYBR&nbsp;Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot SYBR&nbsp;Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#rNaseinhibitor').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#cellLysates').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-cell-lysis-kit-172-5081-desc1.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-cell-lysis-kit-172-5081-desc1.html Storage at –20°C 2199 <strong>12 months:</strong><br /> Lysis buffer<br /> DNase solution<br /> Proteinase K solution 1 0 Storage at 4°C 2757 <strong>6 months:</strong><br />Lysis buffer 1 0 1725038 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5038_1403297461.xml 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml false $2,255.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725081&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725081&CartQuote=QT&quantity= 1450 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725081&AddToDefaultWishList=true&quantity= 12161500 2255.00 1725081
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CFX Automation System II for Real-Time PCR Systems

1845075
Plate handler and barcode scanner, mounting plate, automation software; does not include CFX Real-Time PCR Detection Systems

1845075 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/184-5075_1411499578.xml 1845075 09/23/14 12:11 PM en_US true internet/product_SKU 2019-05-13 20:20:17 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/184-5075_1411499578.xml /default/main/bio-rad/WORKAREA/default jcv3756p 2017-11-14 07:07:39 true jcv3756p templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/184-5075_1411499578.xml LSR N /default/main/bio-rad en 05/13/29 08:56 AM 1556988424838 2019-05-13 20:20:17 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/184-5075_1411499578.xml 1845075-cfx-automation-system-ii-for-real-time-pcr-systems 1 1845075 CFX Automation System II CFX Automation System II for Real-Time PCR Systems Plate handler and barcode scanner, mounting plate, automation software; does not include CFX Real-Time PCR Detection Systems Plate handler and barcode scanner, mounting plate, automation software; does not include CFX Real-Time PCR Detection Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/cfx-automation-system-II-184-5075-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/cfx-automation-system-II-184-5075.jpg CFX Automation System II for Real-Time PCR Systems preview Karen Moss CFX Automation System II Plate handler and barcode scanner, mounting plate, automation software, and manual 1845075, cfx, automation, system, ii, real-time, pcr, detection, system, instrument, machine, real, time, qpcr, high-throughput, high, throughput, robotic, plate, handler, quantitative, gene, expression, genotyping, amplification, 96-well, 384-well 09/23/14 12:11 PM 05/13/29 08:56 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Real-Time-PCR-Detection-System N 0 <p>Combine our robotic plate handler with any CFX Real-Time PCR Detection System to create a high-throughput, automated real-time PCR system with a compact footprint. The CFX Automation System II enables the walk-away generation of large volumes of data with rapid data analysis. The included software is designed to work seamlessly with all CFX Systems, eliminating the need for expertise with automation systems.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Use with one or two CFX Real-Time PCR Detection Systems; mix and match 96- and 384-well systems</li> <li>Add CFX Systems or recalibrate the automation system with the intuitve calibration wizard</li> <li>Customize settings; tailor automation runs to your specific needs</li> <li>Pair cycling information with specific barcoded plates and import barcode information from laboratory information management systems (LIMS)</li> <li>Receive optional email notification with attached data file at the completion of each run</li> </ul> <h2 class="descHeader">Product Components</h2> <ul> <li>Plate handler with attached barcode scanner</li> <li>Mounting plate</li> <li>Calibration block</li> <li>Three removable racks</li> <li>Eight extra gripper pads</li> <li>Two plate lifters for use with CFX384&trade; and CFX384 Touch&trade; Systems</li> <li>CFX Automation Control Software installation CD</li> </ul> <h2 class="descHeader">Configuration Options<img style="padding-bottom: 10px;" src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global//flexible-configurations-meet-your-throughput-needs.jpg" alt="Flexible Configurations Meet Your Throughput Needs" width="597" height="182" /></h2> <p>Flexible <strong>CFX Automation System II</strong> configurations to meet your high-throughput needs.</p> <p>The CFX Automation System II is compatible with all CFX Real-Time PCR Detection Systems:</p> <ul> <li><a href="/en-us/product/cfx96-touch-real-time-pcr-detection-system">CFX96 Touch&trade; Real-Time PCR Detection System</a></li> <li><a href="/en-us/product/cfx96-touch-deep-well-real-time-pcr-detection-system">CFX96 Touch&trade; Deep Well Real-Time PCR Detection System</a></li> <li><a href="/en-us/product/cfx384-touch-real-time-pcr-detection-system">CFX384 Touch&trade; Real-Time PCR Detection System </a></li> <li><a href="/en-us/product/cfx-connect-real-time-pcr-detection-system">CFX Connect&trade; Real-Time PCR Detection System </a></li> </ul> <div id="cfxPrimePCR"> <script type="text/javascript">// <![CDATA[ $('#cfxPrimePCR').load('/webroot/web/html/lsr/products/amplification_pcr/cfx-primepcr.html'); // ]]></script> </div> <h2 class="descHeader">Service Plans and Validation Tools for CFX Systems</h2> <ul> <li>Ensure that your real-time PCR systems are functioning optimally with <a href="/en-us/life-science-research/purchase-service-programs/expert-care-service">Bio-Rad Expert Care Service</a>; choose from several service plans to keep your CFX Automation System II running its best</li> <li>Certify system installation and validate system performance with <a href="/en-us/product/iq-oq-services">CFX System IQ/OQ Services</a> and <a href="/en-us/sku/1845082-iq-oq-kit-service-for-cfx-automation-system-ii">CFX Automation System II IQ/OQ Services </a></li> <li>Verify the performance of your CFX Systems with <a href="/en-us/product/thermal-validation-services">Thermal Validation Services</a> and the <a href="/en-us/product/cfx-qualification-plate">CFX Qualification Plates </a></li> </ul> <h2 class="descHeader">IQ and OQ services offered for the CFX Automation System II</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="319" valign="top"><strong>Services Covered</strong></td> <td width="319" valign="top"> <p><strong>Certified Field Service Engineer Conducts Service</strong></p> </td> <td style="text-align: center;" width="319" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">IQ and OQ</td> <td valign="top">No</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1845080-iq-oq-kit-for-cfx-automation-system-ii">1845080</a></td> </tr> <tr> <td valign="top">OQ</td> <td valign="top">No</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1845081-oq-kit-for-cfx-automation-system-ii">1845081</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">IQ and OQ</td> <td valign="top">Yes</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1845082-iq-oq-kit-service-for-cfx-automation-system-ii">1845082</a></td> </tr> <tr> <td valign="top">OQ</td> <td valign="top">Yes</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1845083-oq-kit-service-for-cfx-automation-system-ii">1845083</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer </a></li> <li>PCR plastics optimized for CFX Real-Time PCR Detection Systems: <ul> <li><a href="/en-us/product/hard-shell-96-well-pcr-plates/hard-shell-96-well-skirted-pcr-plates-low-profile">Hard-Shell<sup>&reg;</sup> 96-Well Low Profile Skirted PCR Plates</a> including barcoded plates (<a href="/en-us/sku/hsp9955-hard-shell-low-profile-thin-wall-96-well-skirted-pcr-plates">HSP9955</a>)</li> <li><a href="/en-us/product/384-well-plates-for-pcr/hard-shell-384-well-skirted-pcr-plates">Hard-Shell 384-Well Skirted PCR Plates</a> including barcoded plates (<a href="/en-us/sku/hsp3905-hard-shell-thin-wall-384-well-skirted-pcr-plates">HSP3905</a>)</li> <li>Microseal<sup>&reg;</sup> 'B' Adhesive Seals (<a href="/en-us/sku/msb1001-microseal-b-adhesive-seals">MSB1001</a>)</li> <li>Optically Clear Heat Seals (<a href="/en-us/sku/1814030-optically-clear-heat-seal">1814030</a>)</li> </ul> </li> <li><a href="/en-us/product/cfx-manager-software-security-edition">CFX Manager Software, Security Edition</a>, for compliance with U.S. FDA 21 CFR Part 11 regulations</li> <li>CFX Qualification Plate for performance verification, 96-well&nbsp; (<a href="/en-us/sku/1845098-cfx-qualification-plate">1845098</a>)</li> <li>CFX Qualification Plate for performance verification, 384-well&nbsp; (<a href="/en-us/sku/1845099-cfx-qualification-plate">1845099</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents">PCR Reagents and qPCR Reagents </a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables </a></li> <li><a href="/en-us/category/pcr-seals">PCR Seals </a></li> <li><a href="/en-us/product/px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer </a></li> <li><a href="/en-us/category/pcr-analysis-software">PCR Analysis Software</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>CFX Automation System II <a href="/webroot/web/pdf/lsr/literature/10042816.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Real-Time PCR Detection Systems <a href="/webroot/web/pdf/lsr/literature/10021337.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>Real-Time PCR <a href="/webroot/web/pdf/lsr/literature/Bulletin_5279.pdf" target="_blank" rel="noopener noreferrer">Applications Guide </a></li> <li>High-Throughput Real-Time PCR Solutions <a href="/webroot/web/pdf/lsr/literature/Bulletin_6629.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li>Real-Time PCR Detection Systems <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/amp_realtime_pcr_systms.html&quot;);">Selection Chart</a></li> <li><a href="/en-us/applications-technologies/qpcr-real-time-pcr">qPCR/Real-Time PCR </a></li> <li><a href="/en-us/applications-technologies/qpcr-instrumentation">qPCR Instrumentation</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global cfx-automation-system-II-184-5075-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/cfx-automation-system-II-184-5075-desc.html Dimensions (W x D x H) 1555 57 x 77 x 97 cm (21 x 30 x 38 in.) 1 0 Weight 2332 55 kg (110 lb) 1 0 Racks 2987 3 1 0 384-well plates per rack 2985 48 1 0 96-well plates per rack 2984 32 1 0 Operating systems 1911 Windows 7, Windows 8 1 0 CFX Manager™ Software 2986 Version 3.1.1621 or higher 1 0 1855484 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/185-5484_1302561154.xml 1814000 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PX1-PCR-Plate-Sealer/181-4000_1347058737.xml false null false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845075&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845075&CartQuote=QT&quantity= 1348 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1845075&AddToDefaultWishList=true&quantity= 41105300 null 1845075
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10000108800 Reliance One-Step Multiplex Supermix Product Insert, Ver A Click to download
7197 Protocol for One-Step Multiplex RT-qPCR Analysis Using PrimePCR Probe Assays and Reliance One-Step Multiplex Supermix Click to download
7175 Reliance One-Step Multiplex Supermix Product Information Sheet, Ver B Click to download
7178 Amplification: Real-Time PCR Reagents and Kits Brochure Click to download
7196 Sensitive Codetection of Viral-Pathogen RNA and DNA Using One-Step Multiplex RT-qPCR Click to download
10000112808 User Manual, Singleshot Cell Lysis Kit Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6629 High-Throughput Real-Time PCR Solutions Brochure Click to download
6763 Gene Expression Real-Time PCR Workflow Flier Click to download [ Add to Cart (Free) ]

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