Reliance One-Step Multiplex Supermix, 1,000 x 20 µl rxns, 5 ml #12010220



5 ml (5 x 1 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 1,000 x 20 μl rxns

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Safety Data Sheet



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Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.

reliance one-step multiplex supermix

Features and Benefits

Exceptional Performance of 5-target Multiplex

Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 µg of RNA input with maximum sensitivity and efficiency.

Unparalleled Room Temperature Stability for Up To 24 Hours

Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.



Efficient Co-detection of RNA and DNA Targets



Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.

View Application Note (PDF 143KB)

Streamline Your Workflow

4x Reliance One-Step Multiplex Supermix is Formulated with:

  • Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**
  • 4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components
  • Reaction stabilizers
  • Patented*** universal reference dye

Applications and Uses

  • Gene expression analysis
  • Viral/pathogen detection (RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)
  • Diagnostic research
  • High-throughput screening (small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)

Packaging Options

Kit Size Catalog Number
1 ml (1 x 1 ml vial), 200 x 20 μl reactions 12010176
5 ml (5 x 1 ml vial), 1,000 x 20 μl reactions 12010220
10 ml (2 x 5 ml vial), 2,000 x 20 μl reactions 12010221

Instrument Compatibility

Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
  • Mx3000P, 3005P, 4000
  • Mastercycler ep realplex 2, 4
  • Corbett Rotor-Gene 3000, 6000, Q
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0

Choose the Right One-Step RT qPCR Product for Your Needs

  Reliance One-Step Multiplex Supermix iTaq Universal One-Step Probe Kit
Key Features
  • Sensitive, efficient amplification of up to 5 targets
  • Room temperature stable up to 24 hours
  • Reliable amplification of up to 2 targets
  • Compatible with a fast protocol
Ideal for
  • Viral/pathogen detection in high-multiplex reaction
  • High throughput screening
  • Viral/pathogen detection
  • Singleplex and duplex reactions

Related Products

Supporting Documents

Online Resources

What is Real-Time PCR (qPCR)?

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* Patent pending
** U.S. patents 7,560,260
*** US patent 9,493,824



Product format
Single-tube, ready-to use supermix
4x concentrated
Detection chemistry
Multiplex capability
Up to 5 targets
Assembled reaction stability
Up to 24 hours
Inhibitor tolerance
Tested with ethanol and hematin. Also compatible with up to 10 µl of crude lysates
5’ to 3’ exonuclease activity of the enzyme(s)
Hot-start, activation time
Yes, 10 min
Sample input
1 pg to 1 µg
10 copies in a 5-plex reaction
Reference dye
Tented universal dye preblended in the supermix
Instrument compatibility
Both ROX-dependent (high- and low-) and -independent instruments
Guaranteed shelf life at –20°
12 months
Shelf life at 4°C (after thawing)
1 week