ReadyPrep™ Protein Extraction Kit (Signal), 50 preps #1632087



50 preps, includes 50 ml signal protein extraction buffer 1 (S1), 50 ml signal protein extraction buffer 2 (S2), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, instruction manual; for 50 extractions of 25–50 mg of cells or tissue

Safety Data Sheet

Safety Data Sheet



 Choose the ReadyPrep Protein Extraction Kit (Signal) to enrich for signaling proteins. This kit uses differential solubilization at 4°C in the presence of Triton X-100 to isolate proteins associated with lipid rafts.

  • Isolate proteins involved in intracellular membrane trafficking and signaling pathways
  • After fractionation, proteins are easily solubilized in the supplied buffer
  • Solubilized proteins can be used immediately in IEF, SDS-PAGE and other applications

Many proteins located within lipid rafts are resistant to solubilization at 4°C by Triton X-100 and other nonionic detergents. The ReadyPrep Protein Extraction Kit (Signal) utilizes a proprietary mixture of nonionic detergents to extract and remove hydrophilic and soluble proteins, leaving behind the signal proteins in a detergent-insoluble fraction.

Proteins that can be isolated using this kit include

  • Gycosylphosphatidylinositol (GPI)-anchored proteins
  • Caveolin and other caveolae-associated proteins
  • Acylated tyrosine kinases
  • Heterotrimeric G-proteins
  • Some proteins with transmembrane domains

Kit Components

For 50 extractions of 25–50 mg of cells or tissue:

  • Signal protein extraction buffer 1 (S1), 50 ml
  • Signal protein extraction buffer 2 (S2), 50 ml
  • Protein solubilization buffer (PSB), 25 g
  • PSB diluent, 30 ml
  • Instruction manual

Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.

ReadyPrep Kit for Fractionation by Cellular Location Proteins Targeted Catalog Number
ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) Cytoplasmic and nuclear 1632089
ReadyPrep Protein Extraction Kit (Membrane I) Membrane-anchored, with one or two transmembrane domains, and hydrophobic 1632088
ReadyPrep Protein Extraction Kit (Membrane II) More than two transmembrane domains and/or proteins resistant to Membrane I Kit 1632084
ReadyPrep Protein Extraction Kit (Signal) Associated with lipid rafts 1632087


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