ProteOn Sensor and Maintenance Chips

ProteOn Sensor and Maintenance Chips

Overview

Used with the ProteOn XPR36 protein interaction array system, ProteOn sensor chips feature advanced surface chemistries for a variety of protein interaction
analysis studies.

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Description

Description

Used with the ProteOn XPR36 protein interaction array system, ProteOn sensor chips combine advanced surface chemistries with state-of-the-art instrument microfluidics. Robust, real-time, label-free biomolecular interaction data are generated using this novel 6 × 6 surface plasmon resonance (SPR) technology.

Features and Benefits of ProteOn Sensor and Maintenance Chips

  • Low cost per data point
  • Range of surfaces designed for diverse protein interaction studies
  • Easy optimization of capture surfaces using validated protocols
  • Maintenance chip and protocols keep your system in excellent working order

Applications and Uses of Surface Plasmon Resonance Technology

ProteOn LCP Sensor Chip
The LCP sensor chip utilizes a NeutrAvidin-coated surface with a planar configuration. This SPR chip is designed for use with the ProteOn LCP reagent kit to capture both single and multiple layers of lipid assemblies such as liposomes.

ProteOn GLC Sensor Chip
The GLC sensor chip is designed for general amine coupling utilizing a compact polymer layer with a binding capacity of approximately one protein monolayer. This SPR chip is most suitable for protein-protein interaction analysis.

ProteOn GLM Sensor Chip
The GLM sensor chip is designed for general amine coupling and has an extended polymer matrix with intermediate binding capacity for high response. This SPR chip is suitable for both protein–small molecule and protein-protein interaction analyses.

ProteOn GLH Sensor Chip
The GLH sensor chip is designed for general amine coupling and has a highly extended polymer layer for maximum binding capacity. This SPR chip is most suitable for protein–small molecule applications in which high sensitivity is the primary objective.

ProteOn NLC Sensor Chip
The NLC sensor chip features NeutrAvidin immobilized on a GLC polymer layer with a binding capacity of approximately one protein monolayer. This SPR chip is an ideal choice for binding biotinylated proteins, peptides, and nucleic acids.

ProteOn HTG and HTE Sensor Chips
These ProteOn sensor chips feature novel tris-NTA complexes for improved capture of polyhistidine-tagged proteins. The complexes are attached to an alginate matrix layer and are activated by injection of Ni2+ ions and regenerated by injection of EDTA.

  • ProteOn HTG sensor chip — has a lower tris-NTA surface density and less nonspecific binding, making it suitable for protein-protein and protein-peptide interaction analysis
  • ProteOn HTE sensor chip — has an elevated tris-NTA surface density optimal for protein–small molecule interaction analysis

Each ProteOn sensor chip is enclosed in a bar-coded cartridge for reliable data tracking and archiving.

ProteOn Maintenance Chips
There are two maintenance sensor chips for use in the ProteOn XPR36 system. These chips are available individually or as part of the ProteOn maintenance kit:

  • Maintenance (MNT) chip — used in maintenance protocols
  • Cleaning (CLN) chip — used in the microfluidics cleaning protocol

The ProteOn XPR36 protein interaction analysis system includes all of the components required for interaction analysis: instrumentation, ProteOn Manager Software, ProteOn sensor chips, buffer and reagent kits, and protocol development kits.

Documents

Documents

Number Description Options
6132
The ProteOn HTG Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Protein Interaction Analysis, Rev A
6295
ProteOn™ Sensor Chips, Tips and Techniques, Rev A
5404
ProteOn™ Sensor Chips Product Information Sheet, Rev F
5409
Protein Interaction Analysis Ordering Information Sheet, Rev E
10024332
ProteOn Liposome Capturing Kit Product Insert, Rev A
5948
Attachment of Membrane Protein Interactions Using Lipoparticles Bound to the Surface of the ProteOn XPR36 Sensor Chips, Rev A
5968
Screening and Characterization of Human Immunoglobulin G Binding Peptides Using the ProteOn XPR36 Protein Interaction Array System, Rev A
5679
Applications of the ProteOn GLH Sensor Chip: Interactions Between Proteins and Small Molecules, Rev A
5540
Screening, Ranking, and Epitope Mapping of Anti-Human IL-9 Supernatants, Rev A
5449
Applications of the ProteOn NLC Sensor Chip: Antibody-Antigen, DNA-Protein, and Protein-Protein Interactions, Rev B
5358
Mechanisms of Protein-Protein Binding: Double-Mutant Cycle Analysis Using the ProteOn XPR36 System, Rev A
5368
Anaylsis of Multiple Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev B
5367
Rapid Optimization of Immobilization and Binding Conditions for Kinetic Analysis of Protein-Protein Interactions Using the ProteOn™ XPR36 Protein Interaction Array System, Rev A
5360
Rapid and Detailed Analysis of Multiple Antigen-Antibody Pairs Using the ProteOn XPR36 Protein Interaction Array System, Rev A
5413
ProteOn XPR36 Hardware Product Information Sheet, Rev B
5412
Antibody Characterization and Development: ProteOn XPR36 Protein Interaction Array System Application Guide, Rev C
6161
Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn XPR36 Protein Interaction Array System, Rev A
6254
The ProteOn™ HTE Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Small Molecule Interaction Analysis, Rev A
6449
Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction using the ProteOn XPR36 System, Rev A
6414
ProteOn™ XPR36 Experimental Design and Application Guide, Rev B