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Description
Description
Nuvia cPrime hydrophobic cation exchange media are a new addition to Bio-Rad's family of mixed-mode purification products. These media are designed for process-scale purification of a wide variety of therapeutic proteins. Nuvia cPrime media's unique selectivity allows method developers to use hydrophobic and cation exchange interaction modes to achieve effective purification. More importantly, the media have a large design space for binding and elution, allowing for the development of highly robust methods in a commercial manufacturing setting. Nuvia cPrime media are built on a rigid, mechanically and chemically stable macroporous base matrix with a particle size optimized to deliver exceptional flow properties, fast mass transfer, and stability.
Nuvia cPrime delivers value by providing:
- New and unique selectivity
- Salt tolerant media
- Simple method development
- Large design space for capture and elution of a variety of biotherapeutic proteins
- High recovery of target protein
- Mechanical and chemical stability
Specifications
Specifications
Functional group
Hydrophobic weak cation exchange
Base matrix composition
Macroporous highly crosslinked polymer
Particle size
70 µm ± 10 µm
Dynamic binding capacity
hlgG: >40 mg/ml (at 10% breakthrough, 300 cm/hr)
Lactoferrin: >60 mg/ml
Lactoferrin: >60 mg/ml
Ligand density
103–143 µeq/ml
Recommended maximum linear flow rate
50–600 cm/hr
Pressure vs. flow performance
Under 2 bar at a flow rate of 600 cm/hr
(20 cm × 20 cm packed bed; 1.17 comp. factor)
(20 cm × 20 cm packed bed; 1.17 comp. factor)
pH stability short-term
2–14
pH stability long-term
3–13
Chemical stability
1.0 N NaOH, 1.0 N HCl, 25% HOAc, 8 M urea, 6 M GuHCl, 6 M KSCN, 3 M NaCl, 1% Triton X-100, 2% SDS + 0.25 M NaCl, 20% EtOH, 70% EtOH, 30% IPA
Shipping solution
20% ethanol with 30 mM sodium sulfite
Storage conditions
20% ethanol
Shelf life in 20% ethanol
5 years at room temperature
Ordering
Ordering
items
Use the filters below to refine results!
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(Discontinued) EconoFit Nuvia cPrime Column, 1 x 1 ml
12009281
Pkg of 1, 1 ml, prepacked hydrophobic cation exchange column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system
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(Discontinued) Nuvia™ cPrime™ Media
1563401
25 ml, hydrophobic cation exchange chromatography media, 70 µm particle size
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(Discontinued) Nuvia™ cPrime™ Media
1563403
500 ml, hydrophobic cation exchange chromatography media, 70 µm particle size
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(Discontinued) Nuvia™ cPrime™ Media
1563402
100 ml, hydrophobic cation exchange chromatography media, 70 µm particle size
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(Discontinued) Nuvia™ cPrime™ Media
156-3404
1 L, hydrophobic cation exchange chromatography media, 70 µm particle size
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(Discontinued) Nuvia™ cPrime™ Media
156-3405
5 L, hydrophobic cation exchange chromatography media, 70 µm particle size
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(Discontinued) Nuvia™ cPrime™ Media
156-3406
10 L, hydrophobic cation exchange chromatography media, 70 µm particle size
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(Discontinued) Foresight™ Nuvia™ cPrime™ Column
7324722
Pkg of 1, 1 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
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(Discontinued) Foresight™ Nuvia™ cPrime™ RoboColumn Unit
7324808
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
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(Discontinued) Foresight™ Nuvia™ cPrime™ RoboColumn Unit
7324807
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
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(Discontinued) Foresight™ Nuvia™ cPrime™ Plates
7324705
2 x 96-well plates, 20 μl, filter plate prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
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(Discontinued) Foresight™ Nuvia™ cPrime™ Column
7324742
Pkg of 1, 5 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media
Documents
Documents
Login Required Number Description Options
7392
Process Resin Solutions for Vaccines and Therapeutics
3218
A Blueprint for Robust, Cost- Efficient Biosimilar Purification Using Ion Exchange Resins
6895
Resin Functionalities Maximize Process Value For Biomolecule Purifications
7128
Using Design of Experiments (DOE) with Nuvia cPrime to Elucidate Capture Conditions of Recombinant Proteins
7130
Improved Process Economics of HUMIRA Biosimilar Purification with Ion Exchange and Mixed-Mode Resins Application Note
7382
Purification of a Recombinant Monoclonal Antibody — a Rituximab Biosimilar — with a Non–Affinity Based Chromatographic Process
10023853
Instruction Manual, Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin, Rev B
6242
Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin Product Information Sheet, Rev C
6992
Single-Step Purification of Raw Starch Digesting α-Amylase Using Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin
6418
Purification of Recombinant Proteins on Nuvia cPrime Hydrophobic Cation Exchange Resin: A Simple Approach to Method Development, Rev A
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6791
Virus Purification Resin Selection Card, Ver A
6792
Aggregate Purification Resin Selection Card, Ver A
6241
A Purification Strategy for Clinical-Grade Monoclonal Antibody Using Hydrophobic Cation Exchange Chromatography, Rev A
6719
Development of an Efficient Manufacturing Process for Adenovirus, Ver A
6720
Development of a cGMP-Ready Purification Process for Adenovirus Purification (Poster), Rev A
6875
Practical Guide: Selecting the Optimal Resins for Monoclonal Antibody Process Purification, Ver A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6813
Practical Guide: Selecting the Optimal Resins for Endotoxin Depletion in Process Purification, Ver A
6807
Practical Guide: Selecting the Optimal Resins for Adenovirus Process Purification, Ver A
6808
Practical Guide: Selecting the Optimal Resins for Aggregate Removal, Ver A
6790
Practical Guide: Selecting the Optimal Resins for Mammalian Virus Process Purification and Clearance, Ver A
6881
Practical Guide: Selecting the Optimal Resins for Removal of DNA Contamination during Process Purification, Ver A
6946
Monoclonal Antibody Purification: Polish Purification Resins Brochure, Ver A
6887
Quick Monoclonal Antibody Purification Strategy: Non-Affinity Based Capture Flier, Ver A
6880
Quick Virus Purification Strategy: Adenovirus Purification Flier, Ver A
6869
Nuvia™ Chromatography Resins Publications List, Ver A
