CHT Ceramic Hydroxyapatite Type II Media

Description

Description

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FREE Ceramic hydroxyapatite reference wall charts

Free Ceramic Hydroxyapatite reference wall charts for process purification

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CHT Ceramic Hydroxyapatite (Ca5(PO4)3OH)2 support is a leading purification medium for today's demanding downstream process industry. This mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT media for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.

The robust properties of CHT Ceramic Hydroxyapatite improve efficiency, yield, and financial value through:

  • Excellent capture at elevated flow rates, enabling processing at all scales
  • Large capacity for high-titer upstream feedstocks
  • Exceptional selectivity, allowing a two-step chromatographic process

Two types of CHT Ceramic Hydroxyapatite, Type I and Type II, are available in three particle sizes, 20, 40, and 80 µm. Both types have elution characteristics similar to crystalline hydroxyapatite but also have some important differences:

  • Type I, sintered at 400°C, has a higher protein binding capacity and better capacity for acidic proteins
  • Type II, sintered at 700°C, has a lower protein binding capacity but offers better resolution of nucleic acids and certain proteins; it also has a very low affinity for albumin and is particularly well-suited for the purification of many species and classes of immunoglobulins
FREE Ceramic hydroxyapatite reference wall charts

Learn Best Practices and Guidelines for Packing CHT Media

These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results. Please download the CHT Application Guide for specific details on recommended buffers and slurry calculations.

Watch Videos | Download Application Guide


Learn How CHT™ Media Can Maximize Purity and Recovery in Downstream Processing

Learn How CHT Media Can Maximize Purity and Recovery in Downstream Processing

This whiteboard animation video provides a brief introduction to Bio-Rad’s innovative mixed-mode chromatography media, CHT Ceramic Hydroxyapatite. The unique separation properties and selectivity of CHT Media enable you to maximize purity and recovery in a single step of downstream purification for the development of biotherapeutics.

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Supporting Documentation

How CHT Ceramic Hydroxyapatite Works Tech Note

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Ordering

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1582200
10 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 20 μm particle size
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1572000
100 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 20 μm particle size
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157-2100
1 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 20 μm particle size
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157-2500
5 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 20 μm particle size
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1584200
10 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 40 μm particle size
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1574000
100 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 40 μm particle size
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157-4100
1 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 40 μm particle size
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157-4500
5 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 40 μm particle size
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1588200
10 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 80 μm particle size
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1578000
100 g, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 80 μm particle size
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157-8100
1 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 80 μm particle size
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157-8500
5 kg, CHT ceramic hydroxyapatite multimodal chromatography media, Type II, 80 μm particle size
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7324718
2 x 96-well plates, 20 μl, filter plate prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size
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7324825
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size
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7324826
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size
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7324736
Pkg of 1, 1 ml, column prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size
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7324756
Pkg of 1, 5 ml, column prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size

Kits & Reagents

Kits & Reagents

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(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm
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7324334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II
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7324332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

Documents

Documents

Number Description Options
7392
Process Resin Solutions for Vaccines and Therapeutics
6895
Resin Functionalities Maximize Process Value For Biomolecule Purifications
[ Add to Cart (Free) ]
6966
Development of a Non–Affinity Based Purification Platform for Neutral/Basic IgMs
7127
Purification of an Acidic IgM with a Strong Anion Exchange Resin Tailored for Large Biomolecule Purification
[ Add to Cart (Free) ]
7112
Acidic IgM Purification with the Strong Anion Exchanger, Nuvia-HP-Q Resin (Poster)
6955
Non–Affinity Based Purification Platform for Neutral/Basic IgMs (Poster)
6086
Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up
[ Add to Cart (Free) ]
6749
Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes, Ver A
[ Add to Cart (Free) ]
6549
Mammalian Virus Purification Using Ceramic Hydroxyapatite, Rev A
[ Add to Cart (Free) ]
2940
Removal of Aggregate from an IgG4 Product Using CHT™ Ceramic Hydroxyapatite Resin, Rev B
5709
How CHT Ceramic Hydroxyapatite Works, Rev A
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5739
Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev A
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2731
Plasmid Purification Using CHT Ceramic Hydroxyapatite Support, Rev A
5667
CHT Ceramic Hydroxyapatite Product Information Sheet, Rev B
[ Add to Cart (Free) ]
2156
CHT Ceramic Hydroxyapatite — A New Dimension in Chromatography of Biological Molecules, Rev C
2780
Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A
2524
Purification of Horse IgG T Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite Type I Supports, Rev C
2774
Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A
1986
Effect of pH on Gradient Elution of Proteins on Two Types of CHT Ceramic Hydroxyapatite, Rev B
1985
Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.
2849
Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A
2535
Purification of Recombinant Human alpha 1-Antitrypsin From Transgenic Milk Using CHT Ceramic Hydroxyapatite, Rev B
2575
Purification of a Calcium-Binding Protein Using CHT Ceramic Hydroxyapatite
2946
Plasmid DNA Adsorption Behavior on CHT Ceramic Hydroxyapatite Type II Support, Rev A
5662
Process Chromatography Applications Laboratory Brochure, Rev B
6278
High-Throughput Calcium Analysis of Hydroxyapatite Column Effluent, Rev A
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
6875
Practical Guide: Selecting the Optimal Resins for Monoclonal Antibody Process Purification, Ver A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6813
Practical Guide: Selecting the Optimal Resins for Endotoxin Depletion in Process Purification, Ver A
6808
Practical Guide: Selecting the Optimal Resins for Aggregate Removal, Ver A
6790
Practical Guide: Selecting the Optimal Resins for Mammalian Virus Process Purification and Clearance, Ver A
6793
Practical Guide: Selecting the Optimal Resins for Immunoglobulin G (IgG) Process Purification, Ver A
6881
Practical Guide: Selecting the Optimal Resins for Removal of DNA Contamination during Process Purification, Ver A
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6791
Virus Purification Resin Selection Card, Ver A
6792
Aggregate Purification Resin Selection Card, Ver A
6889
Novel Selectivities and Workflows to Streamline mAb Purification Processes, Ver A
6870
Ceramic Apatite-Based Chromatography Media Publications List, Ver A
6946
Monoclonal Antibody Purification: Polish Purification Resins Brochure, Ver A
6902
How Does CHT™ Ceramic Hydroxyapatite Media Work (Poster), Ver A
6932
Good Practices for CHT™ Ceramic Hydroxyapatite Media Usage (Poster), Ver A
6965
Recommendations for CHT™ Ceramic Hydroxyapatite Column Packing (Poster)