Immun-Blot® Colorimetric Kits

Immun-Blot® Colorimetric Kits

Overview

Bio-Rad's HRP-based colorimetric detection systems offer a choice of HRP-conjugated secondary antibodies and HRP-conjugated protein A or protein G using the detection reagent 4-chloro-1-naphthol II (4CN).

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Description

Description

Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on the blot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over background is observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however, the method's single endpoint result does not allow the multiple exposures of chemiluminescence methods. Colorimetric detection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.

Bio-Rad's HRP-based colorimetric detection systems offer a choice of HRP-conjugated secondary antibodies and HRP-conjugated protein A or protein G using the detection reagent 4-chloro-1-naphthol(4CN). Bio-Rad offers three types of kits based on this chemistry for colorimetric HRP detection; individual reagents are also available, including 3,3'-diaminobenzidine (DAB), an alternative reagent.

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1708238
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)
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1708240
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, antibody conjugate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)
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1706463
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
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1706464
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays
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1706431
Colorimetric HRP substrate reagent kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution

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