Green Fluorescent Protein Chromatography Kit

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Description

Description

The Green Fluorescent Protein Chromatography Kit can be used following the pGLO™ Bacterial Transformation Kit, which is purchased separately. A colony of transformed bacteria (produced using the pGLO Bacterial Transformation Kit) is placed in liquid cultures overnight, then lysed to release the bacteria's cellular contents. GFP is purified from the bacterial lysate using hydrophobic interaction chromatography (HIC) columns provided in this kit.

Features and Benefits

  • Aligns with AP Biology Lab 6
  • Fluorescent GFP allows real-time monitoring of the extraction and purification processes
  • Uses bacteria transformed with the pGLO Bacterial Transformation Kit

How It Works

Green fluorescent protein is extremely hydrophobic compared to bacterial proteins. Unique characteristics of GFP enable it to be purified from bacterial cell proteins using HIC columns. When placed in a buffer containing a high concentration of salt, the HIC matrix selectively binds hydrophobic GFP molecules while allowing the bacterial proteins to pass through the column. Then, simply lowering the salt concentration of the buffer causes GFP to elute from the column in pure form. pGLO bacterial transformation and GFP purification together explore key processes used in biotechnology to produce and purify "designer proteins" with commercial or research value.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
Ampicillin, lyophilized
1
Arabinose, lyophilized
1
Lysozyme, lyophilized
1
LB nutrient broth tablet
1
Binding buffer
1
Column equilibration buffer
1
Column wash buffer
1
Elution buffer
1
HIC chromatography columns
8
Inoculation loops, sterile
20
Disposable plastic transfer pipets
40
Micro test tubes, 2.0 ml, clear
30
Cell culture tubes, 15 ml, sterile
25
Sample collection tubes, 5 ml
25
Foam micro test tube holders
8
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

Ordering

Ordering

items

Use the filters below to refine results!

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1660005EDU
Protein purification classroom kit, includes lyophilized reagents, nutrient broth tablet, buffers, HIC columns, pipets, test tubes, curriculum, and more, for 32 students; education use only

Refills

Refills

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1660015EDU
Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only
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166-0016EDU_tn.jpg
1660016EDU
Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only
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166-0413EDU_tn.jpg
1660413EDU
Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only
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(Discontinued) Poly-Prep Columns
7311550EDU
Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only

Documents

Documents

Number Description Options
3053
Green Fluorescent Protein Chromatography Kit, Biotechnology Explorer, Quick Guide
4006099
Instruction Manual, Green Fluorescent Protein (GFP) Purification Kit (pGLO Transformation Module 2), Biotechnology Explorer, Rev C