Preamplification Supermix

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Preamplification Supermix

Overview

Sso7d Technology
Exclusive Sso7d Fusion Polymerase Technology to Empower Your PCR

Sso7d, a 7 kD protein obtained from Sulfolobus solfataricus, binds to dsDNA without any sequence specificity.

Sso7d is covalently linked to a DNA polymerase and is able to stabilize the polymerase-template complex without compromising the structural integrity, thermal stability, or catalytic activity of the enzyme.

The unique structure of this Sso7d fusion polymerase provides several significant advantages over ordinary DNA polymerase:

  • Increased processivity
  • Effective amplification in GC-rich regions or strong secondary structure
  • Efficient amplification of longer products
  • Superior tolerance to PCR inhibitors
Sso7D Technology
High-Quality Gene Expression from Precious Samples
High-Quality Gene Expression from Precious Samples — A Simplified Workflow

Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. See how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad's SingleShot™ Cell Lysis Kit, SsoAdvanced™ PreAmp Supermix, and PrimePCR™ Validated Primer Panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.

Unbiased Preamplification
Optimal Unbiased Preamplification of up to 100 Targets

SsoAdvanced™ PreAmp Supermix provides optimal unbiased preamplification of up to 100 targets.

Preamplification of 100 sequences was performed using PrimePCR™ Assays (SYBR® Green) ΔΔCq <0.75.


Cq values (expression levels) of 100 targets used in the preamplification pool shown above.


Beats the Competition
Superior Unbiased Preamplification

SsoAdvanced™ PreAmp Supermix provides superior unbiased preamplification of 24 probe-based qPCR assays compared to Life Technologies TaqMan PreAmp Master Mix.

Preamplification of 24 PrimePCR™ probe-based cancer gene targets using SsoAdvanced PreAmp Supermix followed by qPCR using SsoAdvanced Universal Probes Supermix; average absolute ΔΔCq = 0.21 (1 of 24 >0.75).


Preamplification of 24 TaqMan probe-based cancer gene targets using TaqMan PreAmp Master Mix followed by qPCR using TaqMan Gene Expression Master Mix; average absolute ΔΔCq = 0.48 (6 of 24 > 0.75). Ideal ΔΔCq = 0.00. Cq, quantification cycle.


Low Template Input with Stem Cells
Optimal Preamplification of Low cDNA Input

SsoAdvanced™ PreAmp Supermix provides optimal preamplification of low cDNA input.

NANOG (A, a high expressor), PAX6 (B, a medium expressor), and NEUROG2 (C, a low expressor) show consistent expression levels regardless of the cDNA input (10 ng to 100 pg). cDNA was prepared from a panel of 22 neuronal stem cells treated with retinoic acid for up to 7 days. 1 µg cDNA was used for the no preamplification control.


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SsoAdvanced™ PreAmp Supermix is a 2x concentrated, ready-to-use reaction master mix optimized for unbiased, target-specific preamplification of cDNA and genomic DNA. This PreAmp Supermix preamplifies DNA templates using up to 100 PrimePCR™ PreAmp SYBR® Green or probe-based qPCR assays, custom-designed SYBR® Green and probe-based assays (5’ nuclease), and TaqMan Assays.

  • Limited template DNA — start with as little as 100 pg of cDNA or genomic DNA from any precious or limited sample such as stem cells, laser capture microdissections, and formalin-fixed paraffin-embedded tissues (FFPE)
  • Versatile usability and applications — perform real-time or end-point PCR (SNP genotyping) on up to 100 SYBR® Green or probe-based targets with a simple, streamlined workflow
  • Compatible with various assays — PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Assays

Preamplification of Targets without Bias Is Key

To obtain valid real-time PCR and end-point PCR data from limited samples, preamplification is often required. Some methods have been shown to introduce bias across the transcriptome/genome when comparing preamplified and nonpreamplified samples. The SsoAdvanced PreAmp Supermix is the first supermix that truly provides unbiased preamplification reactions regardless of the targets present in the reaction master mix, enabling more data to be obtained from a limited source.

Competitor Product Comparison

Product ΔΔCq* Chemistry Reactions Price**
SsoAdvanced PreAmp Supermix 90% within ± 0.75 SYBR® Green and probes 50 x 50 µl Least expensive
TaqMan PreAmp Master Mix 90% within ± 1.5 Probes only 40 x 50 µl Most expensive
PerfeCTa PreAmp 5x Supermix 90% within ± 1.5 SYBR® Green and probes 40 x 50 µl More expensive

* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.

Simple Workflow

Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.

Simple Workflow for SsoAdvanced PreAmp Supermix

Recommended RT-qPCR Reagents and Assays

Reverse Transcription

qPCR Supermixes

PreAmp and qPCR Assays

Related Categories

Online Resources

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 3 months
Customer guarantee (minimum usage time) at –20°C 12 months
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SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml

1725160
1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 µl reactions

1725160 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml 1725160 11/22/13 09:02 AM en_US true internet/product_SKU 2019-05-04 16:19:24 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml /default/main/bio-rad/WORKAREA/default jcihuc1z 2017-11-14 07:07:36 true jcihuc1z templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml LSR N /default/main/bio-rad en 11/22/23 09:07 AM 1555974592289 2019-05-04 16:19:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5160_1385140180.xml 1725160-ssoadvanced-preamp-supermix-50-x-50-ul-rxns-1-25-ml 1 1725160 SsoAdvanced PreAmp Supermix SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml 1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 &micro;l reactions 1.25 ml (1 x 1.25 ml), 2x PreAmp Mix contains dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components, for 50 x 50 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-preamp-supermix-172-5160-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-preamp-supermix-172-5160.jpg SsoAdvanced PreAmp Supermix preview ,VN Karen Moss SsoAdvanced PreAmp Supermix 1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 &micro;l reactions 1725160, ssoadvanced, preamp, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, dNTPs, sso7d, fusion, polymerase, mgcl2 11/22/13 09:02 AM 11/22/23 09:07 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SsoAdvanced PreAmp Supermix</strong> to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.</p> <ul> <li>Start with as little as 100 pg of cDNA &mdash;&nbsp;amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)</li> <li>Compatible with PrimePCR&trade; PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays</li> <li>For gene expression and genotyping applications &mdash; validated with <a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; PreAmp Assays</a>, self-designed assays, and TaqMan Assays</li> </ul> <h2 class="descHeader">Product Comparison</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Product</strong></td> <td><strong>&Delta;&Delta;Cq*</strong></td> <td><strong>Chemistry</strong></td> <td><strong>Reactions</strong></td> <td><strong>Price**</strong></td> </tr> <tr class="pd_colorbackground"> <td>SsoAdvanced PreAmp Supermix</td> <td>90% within &plusmn; 0.75</td> <td>SYBR<sup>&reg;</sup> Green and probes</td> <td>50 x 50 &micro;l</td> <td>Least expensive</td> </tr> <tr> <td>TaqMan PreAmp Master Mix</td> <td>90% within &plusmn; 1.5</td> <td>Probes only</td> <td>40 x 50 &micro;l</td> <td>Most expensive</td> </tr> <tr class="pd_colorbackground"> <td>PerfeCTa PreAmp 5x Supermix</td> <td>90% within &plusmn; 1.5</td> <td>SYBR<sup>&reg;</sup> Green and probes</td> <td>40 x 50 &micro;l</td> <td>More expensive</td> </tr> </tbody> </table> <br /> <p class="caption" style="margin-left: 13px;">* Relative quantification between nonamplified and preamplified cDNA.<br /> ** As of May 2014.</p> <h2 class="descHeader">Simple Workflow</h2> <p>Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/simple-workflow-ssoadvanced-preamp-supermix-5-12.jpg" alt="Simple Workflow for SsoAdvanced PreAmp Supermix" width="597" height="278" /></p> <h2 class="descHeader">Recommended RT-qPCR Reagents and Assays</h2> <p>Reverse Transcription</p> <ul> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-reverse-transcription-supermix-for-rt-qpcr">iScript&trade; Reverse Transcription Supermix for RT-qPCR</a></li> <li><a href="/en-us/product/reverse-transcription-reagents/iscript-advanced-cdna-synthesis-kit-for-rt-qpcr">iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR</a></li> </ul> <p>qPCR Supermixes</p> <ul> <li><a href="/en-us/product/ssoadvanced-universal-sybr-green-supermix">SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix</a></li> <li><a href="/en-us/product/ssoadvanced-universal-probes-supermix">SsoAdvanced&trade; Universal Probes Supermix</a></li> </ul> <p>PreAmp and qPCR Assays</p> <ul> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; PreAmp Assays</a></li> <li><a href="/en-us/product/pcr-primers-probes-panels">PrimePCR&trade; Assays</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Find out more about the features of the <a href="/en-us/product/ssoadvanced-preamp-supermix">SsoAdvanced&trade; PreAmp Supermix</a>.</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ssoadvanced-preamp-supermix-172-5160-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ssoadvanced-preamp-supermix-172-5160-desc.html 1 0 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml 1708841 /templatedata/internet/product_SKU/data/ja_JP/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml false £453.00 false $ null true false true true false GBP true true true /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&quantity= false false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&CartQuote=QT&quantity= 1450 false true false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725160&AddToDefaultWishList=true&quantity= 41106300 453.00 1725160
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Number Description Options
10041826 SsoAdvanced PreAmp Supermix Product Insert, Rev C Click to download
6576 SsoAdvanced™ PreAmp Supermix and PrimePCR™ PreAmp Assays Application Note, Rev D Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6777 Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR Click to download
6816 Protocol for Multiplex RT-qPCR Analysis Using PrimePCR™ Assays, Ver A Click to download
6833 High Throughput Single Cell Gene Expression Profiling by Multiplex qPCR, Ver A Click to download
6684 Quantification of 100 RNA Targets from Limited Samples and Single Cells Click to download

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[arquivo-web-crawler (compatible; heritrix/3.4.0-20190418 +http://arquivo.pt)] Catalog/ProductDetail pageStyleKey preamplification-supermix LSR https://www.bio-rad-antibodies.com https://www.bio-rad.com 10/05/13 01:04 AM en_US true internet/generic 2019-07-01 01:54:37 /default/main/bio-rad/STAGING 1380960299662 AL,DZ,AI,AR,AM,AZ,BH,BD,BA,BW,BN,BG,BF,KH,CM,HR,CY,DO,EG,ER,ET,FO,GF,PF,GA,GE,GH,GP,GU,HT,HN,HU,JM,JO,KZ,KE,XK,KW,LB,LI,MO,MK,MG,ML,MQ,MU,FM,MD,MS,MA,NP,NI,NG,OM,PK,PW,PS,PA,PG,PR,QA,RO,ST,SA,SN,RS,SK,SI,SB,LK,TZ,TG,TO,TN,TR,UG,UA,AE,UZ,VU,VA,VN,EH,YE,BO,BR,CA,CL,CO,CR,EC,SV,GT,MX,PY,PE,TT,US,UY,VE,AU,CN,HK,IN,ID,JP,KR,MY,NZ,PH,SG,TW,TH,AT,BE,CZ,DK,EE,FI,DE,GR,IS,IE,IL,IT,LV,LT,LU,MT,NL,NO,PL,PT,RU,ZA,ES,SE,CH,UK templatedata/internet/generic/data/GNL/page_generic_footer_b2c_1380960409_XX.xml page_generic_footer_b2c /default/main/bio-rad/WORKAREA/default jcc1tp9h 2017-11-14 06:29:33 true jcc1tp9h templatedata/internet/generic/data/GNL/page_generic_footer_b2c_1380960409_XX.xml 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