PDS-1000 | He™ System
The PDS-1000/He system uses a burst of high-pressure helium gas to accelerate nucleic acid–coated gold or tungsten microparticles to velocities necessary to transfect cells, tissues, or organelles.
Overview
PDS-1000/He™ System
The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.
The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells
Key Features
- A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
- Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
- Maximum control
- High reproducibility
PDS-1000 / He™ and Hepta™ Training Video. This video provides an overview of the PDS-1000 / He™ and Hepta™ Biolistic Particle Transfection Systems, including instrument setup, consumable preparation, instrument operation and shut down.
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Specifications
Humidity: 0–95% noncondensing
Humidity: 0–95% noncondensing
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Pkg of 1, voltage converter, for use with PDS-1000/He system on 220–240 V lines
Accessories
Pkg of 1, optimization kit for the PDS-1000/He system, includes 0.25 g each of 0.6 um, 1.0 um, and 1.6 um gold microcarriers, 100 each of 7 rupture disks, 1 package of 500 each of stopping screens and macrocarriers, and 2 7/8" caplugs, for 500 bombardments.
Pkg of 50, stopping screens for use with the Hepta adaptor of the PDS-1000/He systems
