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iMark™ Microplate Absorbance Reader

iMark™ Microplate Absorbance Reader

The iMark microplate absorbance reader offers fast reading speeds, extensive onboard software capabilities, a preprogrammed validation protocol (for the Checkmark reader performance check plate).

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Overview

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The iMark™ Microplate Absorbance Reader, with a wavelength range of 400–750 nm, is an economical, high-performance solution for a wide range of applications, including immunoassays with colorimetric substrates, such as ELISA, and protein assays such as Bradford and Lowry. The instrument can be used as a stand-alone reader or be controlled by a PC or Mac with Microplate Manager® 6 Software

Features and Benefits of the iMark Reader

  • Fast microplate reading speeds
  • Extensive onboard assay configuration
  • Powerful data analysis capabilities
  • Convenient built-in shaker
  • Space-saving, wide-format internal thermal graphical printer
  • Simplified standalone operation
  • Compact, easy-to-use, and lightweight design
  • Compatible with different types of 96-well microplates (flat, U-, V-bottom) and 8- or 12-well strip plates; maximum plate height: 16 mm

Features and Benefits of the iMark Reader Onboard Software

  • Wide applicability for life science and clinical laboratories
  • Programmable kinetic reading
  • Security login that allows locking and unlocking of key protocol settings by the administrator
  • Generous memory capacity for protocol creation and storage
  • Comprehensive curve-fit selections
  • Extensive reporting functions
  • Preprogrammed protocols for the Checkmark™ Reader performance verification kit, to validate the IQ/OQ/PQ of the iMark Reader in compliance with GLP
  • Other life science and specialized protocols included such as for the Bio-Rad TSE kits and a growing number of food testing and clinical kits

The iMark Reader comes with six standard absorbance filters: 415, 450, 490, 595, 655, and 750 nm. Bio-Rad also supplies custom filters at 5 nm intervals from 400 to 750 nm on request.

Plate Compatibility

  • Compatible with standard 96-well microplates 128 mm L x 88 mm W
  • Plate height cannot exceed 16 mm
  • Clear flat-bottom plates  are recommended such as Bio-Rad's Costar 96–Well Flat–Bottom EIA Plate (2240096)
  • U-Bottom plates can be used, but V-bottom plates are not recommended

Related Products

Specifications

Wavelength range
400–750 nm
Photometric range
0.0–3.5 OD
Linearity
≤1.0% from 0.0–2.0 OD; ≤2.0% from 0.0–3.0 OD
Accuracy
≤1.0% or 0.010 from 0.000–3.000 OD at 490 nm
Precision
1.0% or 0.005 OD from 0.0–2.0 OD; 1.5% from 2.0–3.0 OD
Resolution
0.001 OD
Filter wheel capacity
8
Plate shaking (3 speeds)
Low, mid, high
Duration, sec
0–999
Read time
6 sec at single wavelength, 10 sec at dual wavelengths
Data output
Onboard graphical thermal printer and USB2 interface with PC or Mac data stations
Data storage
Calender/clock functions; 64 assay protocols
Multilanguage support
4 languages, LCD indication supported; printout report supported
Dimensions (W x D x H), cm (in)
34.6 x 37.7 x 16.4 (13.6 x 14.8 x 6.5)
Weight, kg (lb)
5.5 (12)

Ordering

items

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(Discontinued) Model 1575 Immunowash Microplate Washer
1707009
100/240 V, microplate washer, includes bottle and tubing, 8-port manifold, aerosol protection cover, compatible with flat-, U-, and V-bottom plates

Accessories

(Discontinued) Replacement Lamp for iMark™ microplate absorbance reader
1682006
Pkg of 1, replacement lamp for use with iMark microplate absorbance reader
(Discontinued) Microplate Manager® 6, Version 6.3
1689520
Version 6.3, software intended for collection, analysis, and output of absorbance data from Bio-Rad's iMark™ microplate absorbance reader and xMark™ microplate absorbance spectrophotometer only
(Discontinued) Checkmark™ Reader Performance Verification kit
1686940
Absorbance calibration plate for use with all Bio-Rad absorbance microplate readers, includes Checkmark software to allow GLP compliance

Kits & Reagents

(Discontinued) RC DC™ Protein Assay Kit II
5000122

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, BSA standard; 450 standard assays

(Discontinued) RC DC™ Protein Assay Kit I
5000121

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, bovine γ-globulin standard; 450 standard assays

(Discontinued) Quick Start™ Bradford Protein Assay Kit 4
5000204

Colorimetric assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml)

(Discontinued) Quick Start™ Bradford Protein Assay Kit 2
5000202

Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml)

(Discontinued) Quick Start™ Bradford Protein Assay Kit 1
5000201

Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard (5 x 2 mg/ml); 200 standard assays or 4,000 microplate assays

(Discontinued) DC™ Protein Assay Kit II
5000112
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays
(Discontinued) DC™ Protein Assay Kit I
5000111
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine γ-globulin standard; 450 assays
(Discontinued) Bio-Rad Protein Assay Kit II
5000002

Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine serum albumin standard; for 440 standard assays or 2,200 microplate assays

(Discontinued) Bio-Rad Protein Assay Kit I
5000001

Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine γ-globulin standard; for 440 standard assays or 2,200 microplate assays

(Discontinued) Mouse Typer Isotyping Panel
1722055
Panel includes 10 ml each rabbit anti-mouse subclass-specific antiserum to mouse IgG-1, IgG-2a, IgG-2b, IgG-3, IgM, IgA, κ- and λ-chains, sufficient for 100 typings
(Discontinued) Mouse Typer Isotyping Kit
1722051
Antibody isotyping kit for ELISA, sufficient for 100 typings (800 tests), includes mouse isotyping panel (#172-2055), affinity purified goat anti-rabbit IgG (H + L)
(Discontinued) Single-Component TMB Peroxidase EIA Substrate Kit, 100 ml
1721068

100 ml, single-component EIA substrate solution, contains TMB and H2O2, detection wavelength 450 nm

(Discontinued) TMB Peroxidase EIA Substrate Kit
1721067
For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm
(Discontinued) TMB Peroxidase EIA Substrate Kit
1721066
Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm
(Discontinued) AP Substrate Kit
1721063
Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml
(Discontinued) Rabbit Anti-Goat IgG (H+L)-AP Conjugate
1721037
1 ml, EIA-grade, enzyme-antibody conjugate
(Discontinued) Rabbit Anti-Goat IgG (H+L)-HRP Conjugate
1721034
1 ml, EIA-grade, enzyme-antibody conjugate
(Discontinued) Goat Anti-Rabbit IgG (H+L)-HRP Conjugate
1721019
1 ml, EIA-grade, enzyme-antibody conjugate
(Discontinued) Rabbit Anti-Sheep IgG (H+L)-HRP Conjugate
1721017
1 ml, EIA-grade, enzyme-antibody conjugate

Filters

(Discontinued) 400 nm Filter
1681010
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 405 nm Filter
1681011
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
(Discontinued) 410 nm Filter
1681012
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 415 nm Filter
1681013
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 420 nm Filter
1681014
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 430 nm Filter
1681016
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 440 nm Filter
1681018
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 445 nm Filter
1681019
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 450 nm Filter
1681020
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 455 nm Filter
1681021
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 460 nm Filter
1681022
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 470 nm Filter
1681024
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 495 nm Filter
1681029
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 500 nm Filter
1681030
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 505 nm Filter
1681031
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 510 nm Filter
1681032
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 515 nm Filter
1681033
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 520 nm Filter
1681034
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 530 nm Filter
1681036
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 535 nm Filter
1681037
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 555 nm Filter
1681041
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 565 nm Filter
1681043
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 570 nm Filter
1681044
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 575 nm Filter
1681045
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 580 nm Filter
1681046
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 585 nm Filter
1681047
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 590 nm Filter
1681048
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 595 nm Filter
1681049
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 605 nm Filter
1681051
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
(Discontinued) 610 nm Filter
1681052
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
(Discontinued) 620 nm Filter
1681054
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
(Discontinued) 625 nm Filter
1681055
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 635 nm Filter
1681057
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
(Discontinued) 640 nm Filter
1681058
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark™ (#168-1130) microplate readers
(Discontinued) 650 nm Filter
1681060
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 655 nm Filter
1681061
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 660 nm Filter
1681062
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 670 nm Filter
1681064
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 680 nm Filter
1681066
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 685 nm Filter
1681067
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 690 nm Filter
1681068
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 695 nm Filter
1681069
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 700 nm Filter
1681070
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 705 nm Filter
1681071
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 710 nm Filter
1681072
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 715 nm Filter
1681073
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 725 nm Filter
1681075
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 730 nm Filter
1681076
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 735 nm Filter
1681077
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 745 nm Filter
1681079
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers
(Discontinued) 750 nm Filter
1681080
Pkg of 1, filter for use with Model 680 (#168-1000) and iMark (#168-1130) microplate readers

Microplates

(Discontinued) Costar 96–Well Flat–Bottom EIA Plate
2240096

Box of 4 packages, 25 high binding enzyme immunoassay plates per package (100 plates), polystyrene

Documents

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